Biochemical detection device with controlled reaction incubation time and method for producing the same
A biochemical detection device with a controlled reaction incubation time includes a substrate; a probe disposed on the substrate; a dissolvable material layer disposed on the substrate, wherein the dissolvable material layer has a first opening defined therein, wherein the probe is received in the first opening; an absorbing material layer disposed on the dissolvable material layer and having a second opening defined therein, wherein the first opening communicates with the second opening and is smaller than the second opening; and a non-dissolvable material layer disposed on an inner face of the second opening of the absorbing material layer and on an exposed top face of the dissolvable material layer.
1. A biochemical detection device with a controlled reaction incubation time, the device comprising:
a substrate;
a probe disposed on the substrate;
a dissolvable material layer disposed on the substrate, wherein the dissolvable material layer has a first opening defined therein, and the probe is received in the first opening;
an absorbing material layer disposed on the dissolvable material layer and having a second opening defined therein, wherein the first opening communicates with the second opening and is smaller than the second opening; and
a non-dissolvable material layer disposed on an inner face of the second opening of the absorbing material layer and on an exposed top face of the dissolvable material layer,
wherein a combination of the second opening and the first opening defines a reaction space, and
wherein when a liquid sample is injected into the reaction space, the dissolvable material layer is dissolved by the liquid sample, and then, after a predetermined time, the liquid sample has been transferred to the absorbing material layer such that a reaction incubation time is terminated.
2. The biochemical detection device of claim 1 , wherein the device further comprises a cover disposed on the absorbing material layer.
3. The biochemical detection device of claim 2 , wherein the cover includes:
an injection hole defined therein; and
an injection flow channel defined therein to communicate between the injection hole and the reaction space.
4. The biochemical detection device of claim 3 , wherein when a washing is required, a washing solution is injected through the injection hole and then the washing solution passes through the injection flow channel to the reaction space to wash the probe.
5. The biochemical detection device of claim 1 , wherein a diameter of the second opening is greater than a diameter of the first opening.
6. The biochemical detection device of claim 5 , wherein the reaction incubation time is controlled by controlling a difference in size between the diameter of the second opening and the diameter of the first opening.
7. The biochemical detection device of claim 1 , wherein the reaction incubation time is adjusted based on a dissolution rate of the dissolvable material layer.
8. A method for manufacturing a biochemical detection device with a controlled reaction incubation time, the method comprising:
preparing an absorbing material layer;
forming a first opening in the absorbing material layer;
attaching a dissolvable material layer onto a bottom of the absorbing material layer;
bonding a non-dissolvable material layer to an inner face of the first opening of the absorbing material layer and to an exposed top face of the dissolvable material layer;
forming a second opening defined in the dissolvable material layer and the non-dissolvable material layer, wherein the second opening is smaller than the first opening; and
adhering a substrate having a probe attached thereto onto a bottom of the dissolvable material layer such that the probe is received in the second opening,
wherein a combination of the second opening and the first opening defines a reaction space, and
wherein when a liquid sample is injected into the reaction space, the dissolvable material layer is dissolved by the liquid sample, and then, after a predetermined time, the liquid sample has been transferred to the absorbing material layer such that a reaction incubation time is terminated.
9. The method of claim 8 , wherein the method further comprises disposing a cover on the absorbing material layer.
10. The method of claim 9 , wherein the cover includes:
an injection hole defined therein; and
an injection flow channel defined therein to communicate between the injection hole and the reaction space.
11. The method of claim 10 , wherein when a washing is required, a washing solution is injected through the injection hole and then the washing solution passes through the injection flow channel to the reaction space to wash the probe.
12. The method of claim 8 , wherein a diameter of the first opening is greater than a diameter of the second opening.
13. The method of claim 12 , wherein the reaction incubation time is controlled by controlling a difference in size between the diameter of the second opening and the diameter of the first opening.
14. The method of claim 8 , wherein the reaction incubation time is adjusted based on a dissolution rate of the dissolvable material layer.
15. A portable sensor including the biochemical detection device according to claim 1 .
16. A portable sensor including the biochemical detection device according to claim 2 .
17. A portable sensor including the biochemical detection device according to claim 3 .
18. A portable sensor including the biochemical detection device according to claim 4 .
19. A portable sensor including the biochemical detection device according to claim 5 .
20. A portable sensor including the biochemical detection device according to claim 6 .