Immunocompatible chorionic membrane products
Provided herein is a placental product comprising an immunocompatible chorionic membrane. Such placental products can be cryopreserved and contain viable therapeutic cells after thawing. The placental product of the present invention is useful in treating a patient with a tissue injury (e.g. wound or burn) by applying the placental product to the injury. Similar application is useful with ligament and tendon repair and for engraftment procedures such as bone engraftment.
1. A cryopreserved chorionic membrane comprising native therapeutic cells and matrix metalloproteinase-9 (MMP-9), wherein at least 70% of the native therapeutic cells in the chorionic membrane are viable, wherein the viable native therapeutic cells comprise one or more of mesenchymal stem cells (MSCs), stromal cells, and fibroblast cells, and wherein the cryopreserved chorionic membrane is substantially free of CD14+ macrophage cells.
2. The cryopreserved chorionic membrane of claim 1 , comprising: a cryopreserving amount of a cryopreservative; wherein the cryopreserved chorionic membrane comprises a reticular layer and a stromal layer that both comprise viable therapeutic cells, and wherein the cryopreserved chorionic membrane is substantially free of vascularized tissue or vascularized tissue derived immunogenic cells.
3. The cryopreserved chorionic membrane of claim 1 , wherein the cryopreserved chorionic membrane is substantially free of trophoblasts.
4. The cryopreserved chorionic membrane of claim 1 , wherein the cryopreserved chorionic membrane comprises insulin-like growth factor binding protein-1 and adiponectin.
5. The cryopreserved chorionic membrane of claim 1 , wherein the cryopreserved chorionic membrane has been disinfected.
6. The cryopreserved chorionic membrane of claim 1 , wherein the cryopreserved chorionic membrane has a square shape.
7. The cryopreserved chorionic membrane of claim 1 , wherein the cryopreserved chorionic membrane does not include an amniotic membrane.
8. The cryopreserved chorionic membrane of claim 1 , wherein the cryopreserved chorionic membrane has a thickness of 40 to 400 micrometers.
9. The cryopreserved chorionic membrane of claim 1 , wherein the cryopreserved chorionic membrane is substantially free of vascularized tissue or vascularized tissue-derived immunogenic cells.
10. The cryopreserved chorionic membrane of claim 1 , wherein the cryopreserved chorionic membrane comprises CD14+ macrophages in an amount of less than 5% of the amount of CD14+ macrophage cells in an unprocessed chorionic membrane.
11. The cryopreserved chorionic membrane of claim 1 , wherein the cryopreserved chorionic membrane has a width×length dimension of 5 centimeters by 5 centimeters.