Particle for the encapsidation of a genome engineering system
The present invention relates to a retroviral particle comprising a protein derived from the Gag polyprotein, an envelope protein, optionally an integrase and at least two encapsidated non-viral RNAs, the encapsidated non-viral RNAs each comprising an RNA sequence of interest bound to an encapsidation sequence, each encapsidation sequence being recognized by a binding domain introduced into the protein derived from the Gag polyprotein and/or into the integrase, and at least one of said sequences of interest of the encapsidated non-viral RNAs comprises a part coding a nuclease.
1. A retroviral particle, comprising a protein derived from the Gag polyprotein, an envelope protein, optionally an integrase, and at least two encapsidated non-viral RNAs, the encapsidated non-viral RNAs each comprising an RNA sequence of interest bound to an encapsidation sequence, each encapsidation sequence being recognized by a heterologous binding domain introduced into the protein derived from the Gag polyprotein and/or into the integrase, in which the at least two encapsidated non-viral RNAs differ by their sequence of interest, wherein:
at least one of said sequences of interest of the encapsidated non-viral RNAs comprises a part coding for a nuclease, said nuclease being chosen from the group constituted by a nuclease associated with CRISPR system; and
the sequence of interest of the other encapsidated non-viral RNA corresponds to at least one recognition element of a guide RNA.
2. The retroviral particle according to claim 1 , in which the nuclease is Cas9.
3. The retroviral particle according to claim 1 , which further comprises at least a third encapsidated non-viral RNA having a sequence of interest corresponding to a second recognition element of a guide RNA or to an additional guide RNA.
4. The retroviral particle according to claim 1 , comprising a nucleocapsid protein, an envelope protein, optionally an integrase, and at least two encapsidated non-viral RNAs, the encapsidated non-viral RNAs each comprising an RNA sequence of interest bound to an encapsidation sequence, each encapsidation sequence being recognized by a heterologous binding domain introduced into the nucleocapsid protein and/or into the integrase.
5. The retroviral particle according to claim 4 , in which the heterologous binding domain is introduced into the nucleocapsid protein, and a second heterologous binding domain may be introduced into the nucleocapsid and/or into the integrase.
6. The retroviral particle according to claim 1 , comprising a nucleocapsid protein, an envelope protein, optionally an integrase, and at least two encapsidated non-viral RNAs, the encapsidated non-viral RNAs each comprising an RNA sequence of interest bound to an encapsidation sequence, at least one encapsidation sequence being the stem-loop motif of the MS2 bacteriophage repeated 12 times, said stem-loop motif being recognized by the Coat protein of the MS2 bacteriophage introduced into the nucleocapsid protein.
7. The retroviral particle according to claim 6 , wherein the encapsidated non-viral RNAs comprises, as encapsidation sequence, the stem-loop motif of the MS2 bacteriophage repeated 12 times is an RNA coding Cas9, and a second encapsidated non-viral RNA is an RNA corresponding to at least one recognition element of a guide RNA or coding a guide, said encapsidated non-viral RNAs comprising as encapsidation sequence the stem-loop motif of the MS2 bacteriophage repeated 2 times.
8. The retroviral particle according to claim 1 , comprising a nucleocapsid protein, an envelope protein, optionally an integrase, and at least two encapsidated non-viral RNAs, the encapsidated non-viral RNAs each comprising an RNA sequence of interest bound to an encapsidation sequence, at least one encapsidation sequence being the stem-loop motif of the PP7 bacteriophage repeated 2 times, said stem-loop motif being recognized by the Coat protein of the PP7 bacteriophage introduced into the nucleocapsid protein.
9. The retroviral particle according to claim 1 , in which the heterologous binding domain is introduced into the integrase, and a second heterologous binding domain may be introduced into the nucleocapsid and/or into the integrase.
10. The retroviral particle according to claim 1 , which is a lentiviral particle.
11. A composition comprising the particle according to claim 1 .
12. A kit for producing particles according to claim 1 , comprising:
(i) an expression plasmid comprising at least two different non-viral RNA sequences, each RNA sequence comprising a sequence of interest for which an encapsidation sequence is inserted upstream of, downstream of or within this sequence, or, alternatively, a first and a second expression plasmid each comprising a sequence of interest upstream or downstream of which an encapsidation sequence is inserted, at least one of these sequences of interest coding for a nuclease chosen from a group constituted by nucleases associated with CRISPR system, the other sequence of interest corresponding to at least one recognition element of a guide RNA,
(ii) an encapsidation plasmid coding for a protein derived from the Gag polyprotein and/or a chimeric integrase, comprising a binding domain allowing recognition of an encapsidation sequence, and,
(iii) an envelope plasmid coding for an envelope protein.
13. The kit according to claim 12 , further comprising a second encapsidation plasmid coding for:
a protein derived from the wild-type Gag polyprotein, when the first encapsidation plasmid codes for a protein derived from the chimeric Gag polyprotein, and/or
a wild-type integrase, when the first encapsidation plasmid codes for a chimeric integrase.
14. A manufacturing method for producing the particle according to claim 1 , comprising a step of co-transfection of cells with:
(i) an expression plasmid comprising at least two different non-viral RNA sequences, each RNA sequence comprising a sequence of interest for which an encapsidation sequence is inserted upstream of, downstream of or within this sequence, or, alternatively, a first and a second expression plasmid each comprising a sequence of interest upstream or downstream of which an encapsidation sequence is inserted, at least one of these sequences of interest coding for a nuclease chosen from a group constituted by nucleases associated with CRISPR system, the other sequence of interest corresponding to at least one recognition element of a guide RNA,
(ii) an encapsidation plasmid coding for a protein derived from the Gag polyprotein and/or a chimeric integrase comprising a binding domain allowing recognition of an encapsidation sequence, and,
(iii) an envelope plasmid coding for an envelope protein;
and recovery of the supernatant from the transfected cells comprising a plurality of the particle.
15. The method according to claim 14 , in which the step of co-transfection is further carried out with a second encapsidation plasmid coding for:
a protein derived from the wild-type Gag polyprotein, when the first encapsidation plasmid codes for a protein derived from the chimeric Gag polyprotein, and/or
a wild-type integrase, when the first encapsidation plasmid codes for a chimeric integrase.
16. A composition obtained by the method according to claim 14 .
17. A method for generating in cells a DNA double-strand break, a point mutation, a sequence deletion including gene knock-out, a sequence insertion or gene replacement, comprising transducing the cells with the particle according to claim 1 .