Gene therapy
An inhibitor of p53 activation for use in haematopoietic stem and/or progenitor cell gene therapy, preferably wherein the inhibitor is an inhibitor of p53 phosphorylation, more preferably an inhibitor of p53 Serine 15 phosphorylation.
1. A method of haematopoietic stem and/or progenitor cell gene therapy for reducing in vitro or ex vivo apoptosis of human haematopoietic stem and/or progenitor cells transduced by an integration-defective viral vector comprising a therapeutic nucleotide of interest, the method comprising contacting a population of human haematopoietic stem and/or progenitor cells with an inhibitor of p53 activation during in vitro or ex vivo culture, wherein the inhibitor of p53 activation is an ataxia telangiectasia mutated (ATM) kinase inhibitor or a p53 dominant negative peptide, and wherein the inhibition of p53 activation is transient, and wherein the population of cells is contacted with the inhibitor of p53 activation 30 minutes to 4 hours before transducing the population of cells with the integration-defective viral vector.
2. The method of claim 1 , wherein the integration-defective viral vector is an integration-defective lentiviral vector (IDLV) or adeno-associated viral (AAV) vector.
3. The method of claim 1 , wherein the inhibitor of p53 activation is KU-55933 or a derivative thereof; GSE56; KU-60019, CP-466722, Torin 2, CGK 733, or derivatives thereof; or an siRNA, shRNA, miRNA or antisense DNA/RNA.
4. The method of claim 3 , wherein the inhibitor of p53 activation is added to the human haematopoietic stem and/or progenitor cells at a concentration of 1-50 μM.
5. The method of claim 3 , wherein the inhibitor of p53 activation is added to the human haematopoietic stem and/or progenitor cells at a concentration of 5-50 μM.
6. The method of claim 1 , wherein the integration-defective viral vector is an integration-defective lentiviral vector (IDLV) or adeno-associated viral (AAV) vector.
7. The method of claim 1 , wherein the inhibitor of p53 activation is a p53 dominant negative peptide comprising an amino acid sequence having at least 80% identity to SEQ ID NO: 6.
8. The method of claim 1 , wherein the inhibitor of p53 activation is KU-55933, GSE56 or KU-60019.
9. The method of claim 1 , wherein the population of cells is contacted with the inhibitor of p53 activation 2 hours before transducing the population of cells with the integration-defective viral vector.
10. A method of reducing in vitro or ex vivo apoptosis of human haematopoietic stem and/or progenitor cells transduced by an integration-defective viral vector comprising a therapeutic nucleotide of interest, the method comprising contacting a population of human haematopoietic stem and/or progenitor cells with an inhibitor of p53 activation during in vitro or ex vivo culture, wherein the inhibitor of p53 activation is an ataxia telangiectasia mutated (ATM) kinase inhibitor or a p53 dominant negative peptide, and wherein the inhibition of p53 activation is transient, and wherein the population of cells is contacted with the inhibitor of p53 activation 30 minutes to 4 hours before transducing the population of cells with the integration-defective viral vector.
11. The method of claim 10 , wherein the inhibitor of p53 activation is KU-55933 or a derivative thereof; GSE56; KU-60019, CP-466722, Torin 2, CGK 733, or derivatives thereof; or an siRNA, shRNA, miRNA or antisense DNA/RNA.
12. The method of claim 11 , wherein the inhibitor of p53 activation is added to the human haematopoietic stem and/or progenitor cells at a concentration of 1-50 μM.
13. The method of claim 11 , wherein the inhibitor of p53 activation is added to the human haematopoietic stem and/or progenitor cells at a concentration of 5-50 μM.
14. The method of claim 10 , wherein the inhibitor of p53 activation is a p53 dominant negative peptide comprising an amino acid sequence having at least 80% identity to SEQ ID NO: 6.
15. The method of claim 10 , wherein the inhibitor of p53 activation is KU-55933, GSE56 or KU-60019.
16. The method of claim 10 , wherein the population of cells is contacted with the inhibitor of p53 activation 2 hours before transducing the population of cells with the integration-defective viral vector.
17. A method of transducing a population of human haematopoietic stem and/or progenitor cells with an integration-defective viral vector comprising a therapeutic nucleotide of interest, the method comprising:
(a) contacting the population of cells with an inhibitor of p53 activation during in vitro or ex vivo culture, wherein the inhibitor of p53 activation is an ataxia telangiectasia mutated (ATM) kinase inhibitor or a p53 dominant negative peptide, and wherein the inhibition of p53 activation is transient; and
(b) transducing the population of cells with the integration-defective viral vector during in vitro or ex vivo culture,
wherein the population of cells is contacted with the inhibitor of p53 activation 30 minutes to 4 hours before transducing the population of cells with the integration-defective viral vector.
18. The method of claim 13 , wherein the inhibitor of p53 activation is KU-55933 or a derivative thereof; GSE56; KU-60019, CP-466722, Torin 2, CGK 733, or derivatives thereof; or an siRNA, shRNA, miRNA or antisense DNA/RNA.
19. The method of claim 15 , wherein the inhibitor of p53 activation is added to the human haematopoietic stem and/or progenitor cells at a concentration of 1-50 μM.
20. The method of claim 18 , wherein the inhibitor of p53 activation is added to the human haematopoietic stem and/or progenitor cells at a concentration of 5-50 μM.
21. The method of claim 17 , wherein the integration-defective viral vector is a integration-defective lentiviral vector (IDLV) or adeno-associated viral (AAV) vector.
22. The method of claim 17 , wherein the population of human haematopoietic stem and/or progenitor cells is obtained from mobilised peripheral blood, bone marrow or umbilical cord blood.
23. The method of claim 17 , which includes a further step of enriching the population for haematopoietic stem and/or progenitor cells.
24. The method of claim 17 , wherein the inhibitor of p53 activation is a p53 dominant negative peptide comprising an amino acid sequence having at least 80% identity to SEQ ID NO: 6.
25. The method of claim 17 , wherein the inhibitor of p53 activation is KU-55933, GSE56 or KU-60019.
26. The method of claim 17 , wherein the population of cells is contacted with the inhibitor of p53 activation 2 hours before transducing the population of cells with the integration-defective viral vector.
27. A method of gene therapy comprising the steps:
(a) transducing a population of human haematopoietic stem and/or progenitor cells according to the method of claim 17 ; and
(b) administering the transduced cells to a subject.
28. The method of claim 27 , wherein the transduced cells are administered to a subject as part of an autologous stem cell transplant procedure or an allogeneic stem cell transplant procedure.