Method for producing 3-hydroxyadipic acid
Provided is a method of producing 3-hydroxyadipic acid, the method including the step of culturing a microorganism belonging to the genus Serratia capable of producing 3-hydroxyadipic acid.
1. A microorganism belonging to the genus Serratia producing 3-hydroxyadipic acid, wherein an enzymatic activity that catalyzes a reaction to produce 3-oxoadipyl-CoA and CoA from succinyl-CoA and acetyl-CoA is enhanced by introducing a gene encoding a protein having said enzymatic activity to said microorganism belonging to the genus Serratia , or by modifying a functional region(s) in a vicinity of a coding region in a gene encoding a protein having said enzymatic activity in said microorganism belonging to the genus Serratia.
2. The microorganism belonging to the genus Serratia according to claim 1 , wherein said microorganism belonging to the genus Serratia is Serratia grimesii, Serratia ficaria, Serratia fonticola, Serratia odorifera, Serratia plymuthica, Serratia entomophila , or Serratia nematodiphila.
3. A method of producing 3-hydroxyadipic acid, said method comprising the step of culturing a microorganism claimed in claim 1 belonging to the genus Serratia capable of producing 3-hydroxyadipic acid.
4. The method of producing 3-hydroxyadipic acid according to claim 3 , wherein said microorganism belonging to the genus Serratia has an enhanced enzymatic activity that catalyzes a reaction to produce 3-oxoadipyl-CoA and CoA from succinyl-CoA and acetyl-CoA.
5. The method of producing 3-hydroxyadipic acid according to claim 3 , wherein said microorganism belonging to the genus Serratia is Serratia grimesii, Serratia ficaria, Serratia fonticola, Serratia odorifera, Serratia plymuthica, Serratia entomophila , or Serratia nematodiphila.
6. The method of producing 3-hydroxyadipic acid according to claim 3 , wherein a medium for culturing said microorganism belonging to the genus Serratia contains at least one or more carbon sources selected from the group consisting of saccharides, succinic acid, 2-oxoglutaric acid, and glycerol.
7. The method of producing 3-hydroxyadipic acid according to claim 3 , wherein said microorganism belonging to the genus Serratia is cultured in a medium that contains at least one or more inducers selected from the group consisting of ferulic acid, p-coumaric acid, benzoic acid, cis,cis-muconic acid, protocatechuic acid, and catechol.