Anti-CD7 chimeric antigen receptor and method of use thereof
A nucleic acid construct and mammalian cell harboring nucleic acids encoding an anti-CD7 chimeric antigen receptor are provided. Methods for treating cancer, in particular a hematologic cancer, using the nucleic acid construct or mammalian cell are also described.
1. An isolated nucleic acid construct encoding a chimeric antigen receptor (CAR) comprising an anti-CD7 binding domain, a transmembrane domain, and a cytoplasmic domain, wherein said CAR comprises the amino acid sequence of SEQ ID NO:2.
2. The nucleic acid construct of claim 1 , wherein the construct comprises a vector.
3. A mammalian cell comprising the nucleic acid construct of claim 1 .
4. The mammalian cell of claim 3 , wherein the cell is a T cell, a Natural Killer cell, a cytotoxic T lymphocyte, or a regulatory T cell.
5. A method for producing an anti-CD7 chimeric antigen receptor (CAR) T cell comprising
(a) obtaining a population of CD7-negative T cells; and
(b) transducing the CD7-negative T cells with a nucleic acid construct encoding an anti-CD7 CAR comprising the amino acid sequence of SEQ ID NO:2.
6. A method for treating a CD7 expressing hematological cancer comprising delivering to a subject in need of treatment an effective amount of a T cell, a Natural Killer cell, or a cytotoxic T lymphocyte, said cell comprising the nucleic acid construct of claim 1 , thereby treating the subject's cancer.