Cytoplasmic incompatibility factors and methods for controlling anthropods
The disclosure relates to genetically modified bacteria, genetically modified arthropods, and methods for controlling and/or reducing arthropod populations.
1. A genetically modified arthropod, said arthropod comprising:
(i) a bacterial operon or two independent chromosomal insertions encoding at least two cytoplasmic incompatibility factors, wherein the bacterial operon or the two independent chromosomal insertions is from Wolbachia;
wherein the bacterial operon or the two independent chromosomal insertions encode: the cytoplasmic incompatibility factors WD0631 and WD0632; and
wherein WD0631 is encoded by SEQ ID NO: 25; and
(ii) a promoter operably linked to the bacterial operon or the two independent chromosomal insertions;
wherein the expression of the cytoplasmic incompatibility factors in a male arthropod causes a reduction in viable offspring in comparison to a male arthropod lacking the cytoplasmic incompatibility factors.
2. A genetically modified arthropod, said arthropod comprising:
(i) a bacterial operon or two independent chromosomal insertions encoding at least two cytoplasmic incompatibility factors, wherein the bacterial operon or the two independent chromosomal insertions is from Wolbachia;
wherein the bacterial operon or the two independent chromosomal insertions encode:
the cytoplasmic incompatibility factors WD0631 and WD0632; and
wherein WD0632 is encoded by SEQ ID NO: 26; and
(ii) a promoter operably linked to the bacterial operon or the two independent chromosomal insertions;
wherein the expression of the cytoplasmic incompatibility factors in a male arthropod causes a reduction in viable offspring in comparison to a male arthropod lacking the cytoplasmic incompatibility factors.
3. A genetically modified arthropod, said arthropod comprising:
(i) a bacterial operon or two independent chromosomal insertions encoding at least two cytoplasmic incompatibility factors, wherein the bacterial operon or the (wo independent chromosomal insertions is from Wolbachia;
wherein the bacterial operon or the two independent chromosomal insertions encode:
the cytoplasmic incompatibility factors CidA wPip and CidB wPip ; and
wherein CidA wPip is encoded by SEQ ID NO: 27; and
(ii) a promoter operably linked to the bacterial operon or the two independent chromosomal insertions;
wherein the expression of the cytoplasmic incompatibility factors in a male arthropod causes a reduction in viable offspring in comparison to a male arthropod Jacking the cytoplasmic incompatibility factors.
4. A genetically modified arthropod, said arthropod comprising:
(i) a bacterial operon or two independent chromosomal insertions encoding at least two cytoplasmic incompatibility factors, wherein the bacterial operon or the two independent chromosomal insertions is from Wolbachia;
wherein the bacterial operon or the two independent chromosomal insertions encode:
the cytoplasmic incompatibility factors CidA wPip and CidB wPip ; and
wherein CidB wPip is encoded by SEQ ID NO: 28; and
(ii) a promoter operably linked to the bacterial operon or the two independent chromosomal insertions:
wherein the expression of the cytoplasmic incompatibility factors in a male arthropod causes a reduction in viable offspring in comparison to a male arthropod lacking the cytoplasmic incompatibility factors.