Fluid analyzer for measuring a target analyte and method of calibrating an amperometric sensor
A control system for a fluid analyzer is described. The control system has a processor executing processor executable code to: control a potentiostat to apply a first voltage potential sufficient to induce a first electrochemical reaction of a target analyte or a reaction byproduct of the target analyte in a sample of the calibration reagent and receive a first reading from the potentiostat; control the potentiostat to apply a second voltage potential insufficient to induce a second electrochemical reaction of the target analyte or a reaction byproduct of the target analyte in the sample of the calibration reagent and receive a second reading from the potentiostat; calculate calibration parameters using the first reading, the second reading and a multi-point calibration algorithm; and measure a target analyte concentration within the fluid sample using the calibration parameters.
1. A fluid analyzer for analyzing a fluid sample, comprising:
a potentiostat, when in electrical communication with two or more electrodes of an amperometric sensor, configured to measure signals generated by at least two of the two or more electrodes, and to control a voltage difference between at least two of the two or more electrodes;
at least one calibration injection port adapted to receive a calibration reagent having a predetermined target analyte level of a target analyte;
at least one automated valve communicating with the at least one calibration injection port and being openable and closable to pass one or more samples of the calibration reagent; and
a control system having a processor executing processor executable code that when executed causes the processor to:
control the at least one automated valve to pass the calibration reagent through a fluid channel to the two or more electrodes;
control the potentiostat to apply a first voltage potential sufficient to induce a first electrochemical reaction of the target analyte or a reaction byproduct of the target analyte in a sample of the calibration reagent and receive a first reading from the potentiostat;
control the potentiostat to apply a second voltage potential insufficient to induce a second electrochemical reaction of the target analyte or the reaction byproduct of the target analyte in the sample of the calibration reagent and receive a second reading from the potentiostat;
calculate calibration parameters using the first reading, the second reading and a multi-point calibration algorithm; and
measure a target analyte concentration within the fluid sample using the calibration parameters.
2. The fluid analyzer of claim 1 , wherein the target analyte is oxygen, and wherein the predetermined target analyte level is characterized as an oxygen tension within a range from 0 to 800 mmHg.
3. The fluid analyzer of claim 1 , wherein the multi-point calibration algorithm includes a zero target analyte calibration point.
4. The fluid analyzer of claim 1 , wherein the calibration parameters establish a correlation between the first reading, the second reading and known concentrations of the target analyte.
5. The fluid analyzer of claim 1 , wherein the target analyte is a metabolite.
6. The fluid analyzer of claim 1 , wherein the second reading is correlated to a zero analyte concentration level.
7. The fluid analyzer of claim 1 , wherein the fluid sample is a biological sample, and wherein the measurement of the target analyte concentration is configured into a form suitable for a medical diagnostic purpose.
8. The fluid analyzer of claim 1 , wherein the fluid sample is a non-biological sample.
9. The fluid analyzer of claim 1 , wherein controlling the potentiostat to apply the first voltage potential occurs prior to controlling the potentiostat to apply the second voltage potential.
10. The fluid analyzer of claim 1 , wherein controlling the potentiostat to apply the second voltage potential occurs prior to controlling the potentiostat to apply the first voltage potential.
11. The fluid analyzer of claim 1 , wherein the second reading is indicative of non-faradaic current.