Modular platform for targeted therapeutics
A lipidated secondary antibody is disclosed. Particles comprising same are also disclosed.
1. An immunoparticle comprising a lipidated secondary antibody comprising a secondary antibody attached via a linker to a lipidated peptide comprising an inner membrane lipidation signal, said lipidated secondary antibody is non-covalently attached to an outer surface of a synthetic lipid-based nanoparticle comprising synthetic lipids via said lipidated peptide, and attached to a primary antibody via an antigen recognition domain of said secondary antibody, wherein said secondary antibody has specificity for said primary antibody.
2. The immunoparticle of claim 1 , wherein said primary antibody is a humanized or human primary antibody.
3. The immunoparticle of claim 1 , wherein said immunoparticle is loaded with a diagnostic or a therapeutic agent.
4. The immunoparticle of claim 1 , wherein said therapeutic agent is a polynucleotide agent.
5. The immunoparticle of claim 1 , wherein said primary antibody and said secondary antibody are of different antibody classes or antibody isotypes.
6. The immunoparticle of claim 1 , wherein said primary antibody and said secondary antibody are selected from the group consisting of IgG1, IgG2 and IgG4.
7. The immunoparticle of claim 1 , wherein said lipid-based nanoparticle comprises a charged external surface.
8. The immunoparticle of claim 1 , wherein said lipid-based nanoparticle comprises cationic lipids.
9. The immunoparticle of claim 1 , wherein said primary antibody comprises an Fc region and said secondary antibody binds said Fc region of said primary antibody.
10. The immunoparticle of claim 4 , wherein said polynucleotide agent is an siRNA.
11. The immunoparticle of claim 1 , wherein said lipidated peptide comprises SEQ ID NO: 13.
12. The immunoparticle of claim 1 , wherein said lipidated peptide consists of SEQ ID NO: 13.