IP Library Granted Patent US 11,555,197
Granted Patent B2
US 11,555,197 · App. 16/316,783 · Granted Jan 17, 2023

Control of phenotype in plants

Inventors: Sekhar Boddupalli (Davis, CA); Andrey Boukharov (Melrose, MA); Rio Stamler (Davis, CA); Zhongsen Li (Hockessin, DE); Arianne Tremblay (Davis, CA); Stephen Schauer (Rockville, MD); Shiv B. Tiwari (San Jose, CA); John Salmeron (Hillsborough, NC)
Assignee: GREENVENUS, LLC
C12N15/8217C12N15/825C12N15/827C12N15/8218C12N15/8273C12N15/8274C12N15/8281C12N15/8282C12N15/8283C12N15/8286
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Quick Facts
Patent No.
US 11,555,197
App. No.
16/316,783
Granted
Jan 17, 2023
Kind
B2
Abstract

The invention provides a compositions and methods for controlling phenotypic traits in plants. Genes of interest are placed under the control of a gene switch to allow inducible control or expression of a gene of interest “on-demand” by treatment of the plant with a chemical ligand.

Claims (23)

1. One or more vectors or polynucleotides comprising:

(a) a polynucleotide encoding a VGEvy gene switch, comprising:

(i) a transactivation domain;

(ii) a DNA-binding domain; and

(iii) a ligand-binding domain having an amino acid sequence of SEQ ID NO:2;

(b) a nucleic acid which controls one or more traits in a plant; and

(c) a regulatory element operably connected 5′ of (b),

wherein the VGEvy gene switch induces expression of (b) in the presence of a chemical ligand.

2. The one or more vectors or polynucleotides of claim 1 , wherein said one or more traits comprises one or more of flowering, drought resistance, herbicide resistance, pest resistance, diseases resistance, type of floral color, intensity of floral color, floral aroma, accumulation of specific nutrients, taste, aroma, color, taste, nutritional value, or expression of an exogenous gene.

3. The one or more vectors or polynucleotides of claim 2 , wherein said nucleic acid controls flowering and comprises a polynucleotide encoding a full-length polypeptide, or biologically active fragment thereof, of one or more of Gigantea (GI), Nuclear Factor Y (NFY), Constans (CO), protein farnesyltransferase (PFT), Agamous-Like 15 (AGL15), Flowering Locus T (FT), Twin Sister of FT (TSF), bZIP transcription factor (FD), Apetalal (AP1), Suppressor of Overexpression of CO1 (SOC1), Agamous-Like 24 (AGL24); Leafy (LFY), or Squamosa Promoter Binding Protein-Like 3a (SPL3).

4. The one or more vectors or polynucleotides of claim 1 , wherein said DNA-binding domain comprises one or more of a Gal4 binding domain, a LexA binding domain, a transcription factor binding domain, a Group H nuclear receptor member binding domain, a steroid/thyroid hormone nuclear receptor superfamily member binding domain, a bacterial LacZ binding domain, an EcR binding domain, an ARF, a bZIP, a homeodomain family of a DNA binding domain, or synthetic DNA-binding protein.

5. The one or more vectors or polynucleotides of claim 1 , wherein said transactivation domain comprises one or more of a Group H nuclear receptor member transactivation domain, steroid/thyroid hormone nuclear receptor transactivation domain, synthetic or chimeric transactivation domain, polyglutamine transactivation domain, basic or acidic amino acid transactivation domain, a VP16 transactivation domain, a GAL4 transactivation domain, an NF-κB transactivation domain, a BP64 transactivation domain, a B42 acidic transactivation domain (B42AD), a p53 transactivation domain, a p65 transactivation domain (p65AD), an EDLL acidic transactivation domain from AP2/ERF, a glutamine-rich transactivation domain, an ARFs or CONSTANS protein transactivation domain, a proline-rich domain transactivation domain from bZIP, or an analog, combination, or modification thereof.

6. The one or more vectors or polynucleotides of claim 1 , wherein said DNA-binding domain is a GAL4 transcription factor DNA-binding domain and/or wherein said transactivation domain is (i) derived from a Sarcoma Virus 40 (SV40) transcriptional transactivation domain or (ii) comprises a herpes virus VP16 transcriptional transactivation domain.

7. The one or more vectors or polynucleotides of claim 1 , wherein said VGEvy gene switch comprises the polypeptide sequence of SEQ ID NO:1.

8. The one or more vectors or polynucleotides of claim 1 , wherein said chemical ligand is methoxyfenozide or diacylhydrazine.

9. The one or more vectors or polynucleotides of claim 1 , wherein said regulatory element comprises (i) a recognition site for the DNA-binding domain of said VGEvy gene switch and (ii) a promoter.

10. A plant comprising one or more vectors or polynucleotides of claim 1 .

11. The plant of claim 10 , wherein all or a portion of the one or more vectors or polynucleotides is integrated into the plant genome.

12. The plant of claim 11 , wherein said plant is a dicot.

13. A method of controlling or regulating flowering in a plant comprising introducing one or more vectors or polynucleotides of claim 1 into said plant.

14. The method of claim 13 , further comprising contacting said plant with a non-steroidal chemical ligand wherein said chemical ligand is diacylhydrazine or methoxyfenozide.

15. The plant of claim 11 , wherein said plant is a monocot.

16. The method of claim 13 , wherein the plant is alfalfa sprouts, apples, apricots, artichokes, Asian pears, asparagus, atemoyas, avocados, bamboo shoots, bananas, beans, bean sprouts, beets, belgian endive, bitter melons, bell peppers, blackberries, blueberries, bok choy, boniato, boysenberries, broccoflowers, broccoli, broccolini, brussels sprouts, butter lettuce, cabbage, cantaloupe, carambola, carrots, casaba melons, cauliflower, celery, chayotes, cherimoyas, cherries, coconuts, coffee, collard greens, corn, cranberries, cucumbers, dates, eggplant, endive, escarole, feijoa, fennel, figs, garlic, gooseberries, grapefruit, grapes, green beans, green onions, collard greens, mustard greens, guava, hominy, honeydew melons, horned melons, iceberg lettuce, Jerusalem artichokes, jicama, kale, kiwifruit, kohlrabi, kumquats, leeks, lemons, lettuce, lima beans, limes, longan, loquats, lychees, mandarins, malangas, mandarin marijuana, oranges, mangos, mulberries, mushrooms, napas, nectarines, okra, onions, oranges, papayas, parsnip, passion fruits, paw-paws, peaches, peanut, pears, sugar snap peas, green peas, peppers, persimmons, pineapples, plantains, plums, pomegranates, potatoes, prickly pears, pummelos, pumpkins, quince, radicchio, radishes, raspberries, red cabbage, rhubarb, romaine lettuce, rutabaga, shallots, snow peas, soybeans, spinach, sprouts, squash, strawberries, string beans, sweet potatoes, tangelo, tangerines, tomatillo, tomatoes, turnip, ugli fruit, watermelons, water chestnuts, watercress, waxed beans, yams, yellow squash, yuca/cassava, or zucchini squash.

Assignments (5)
CHANGE OF NAME Recorded Jan 21, 2026
From: GREENVENUS, LLC
To: KENDRED, LLC
Reel/Frame 074461/0951 →
CORRECTIVE ASSIGNMENT TO CORRECT THE ADDRESS OF THE RECEIVING PARTY ON THE PATENT ASSIGNMENT COVER SHEET PREVIOUSLY RECORDED AT REEL: 053249 FRAME: 0306. ASSIGNOR(S) HEREBY CONFIRMS THE ASSIGNMENT. Recorded Sep 28, 2020
From: INTREXON CORPORATION
To: PRECIGEN, INC.
Reel/Frame 053899/0219 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 25, 2020
From: PRECIGEN, INC.
To: GREENVENUS, LLC
Reel/Frame 053312/0021 →
CHANGE OF NAME Recorded Jul 20, 2020
From: INTREXON CORPORATION
To: PRECIGEN, INC.
Reel/Frame 053249/0306 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 24, 2019
From: BODDUPALLI, SEKHAR; STAMLER, RIO; BOUKHAROV, ANDREY; LI, ZHONGSEN; TREMBLAY, ARIANNE; SCHAUER, STEPHEN; TIWARI, SHIV; SALMERON, JOHN
To: INTREXON CORPORATION
Reel/Frame 048982/0571 →
Continuity (4)
Provisional Application 62366402 · Jul 25, 2016
Provisional Application 62468012 · Mar 7, 2017
Provisional Application 62467958 · Mar 7, 2017
Related Publication 20190233834A1 · Aug 1, 2019
Cited By (1)
US 12,344,852