Method for measuring glycated hemoglobin
A method is provided for measuring glycated hemoglobin in a hemoglobin-containing sample which comprises reacting glycated hemoglobin in the hemoglobin-containing sample with an enzyme that catalyzes a reaction of oxidizing the glycated hemoglobin to generate hydrogen peroxide, in the presence of at least one anionic surfactant selected from the group consisting of N-acyl taurine in which a hydrogen atom of the amino group may be substituted with a substituent, alkyl sulfoacetic acid, polyoxyethylene alkyl ether acetic acid, N-acyl amino acid in which a hydrogen atom of the amino group may be substituted with a substituent, polyoxyethylene alkyl ether phosphoric acid, polyoxyethylene polycyclic phenyl ether phosphoric acid, alkyl phosphoric acid, and salts thereof, to generate hydrogen peroxide, and measuring the generated hydrogen peroxide.
1. A method for measuring glycated hemoglobin in a hemoglobin-containing sample, comprising:
reacting glycated hemoglobin in the hemoglobin-containing sample with an enzyme that catalyzes a reaction of oxidizing the glycated hemoglobin to generate hydrogen peroxide, in the presence of at least one anionic surfactant selected from the group consisting of N-acyl taurine in which a hydrogen atom of the amino group is optionally substituted with a substituent, alkyl sulfoacetic acid, polyoxyethylene alkyl ether acetic acid, N-acyl amino acid in which a hydrogen atom of the amino group is optionally substituted with a substituent, polyoxyethylene alkyl ether phosphoric acid, polyoxyethylene polycyclic phenyl ether phosphoric acid, alkyl phosphoric acid, and salts thereof; and
measuring the generated hydrogen peroxide.
2. The method according to claim 1 , wherein the measurement of the hydrogen peroxide is carried out with a reagent for measuring hydrogen peroxide.
3. The method according to claim 2 , wherein the reagent for measuring hydrogen peroxide is a reagent containing a peroxidase and a leuco-type chromogen.
4. The method according to claim 3 , wherein the leuco-type chromogen is a phenothiazine-based chromogen.
5. The method according to claim 4 , wherein the phenothiazine-based chromogen is 10-(carboxymethylaminocarbonyl)-3,7-bis(dimethylamino)phenothiazine or a salt thereof.
6. The method according to claim 1 , wherein the method comprises
reacting the glycated hemoglobin in the hemoglobin-containing sample with at least one anionic surfactant selected from the group consisting of N-acyl taurine in which a hydrogen atom of the amino group may be substituted with a substituent, alkyl sulfoacetic acid, polyoxyethylene alkyl ether acetic acid, N-acyl amino acid in which a hydrogen atom of the amino group may be substituted with a substituent, polyoxyethylene alkyl ether phosphoric acid, polyoxyethylene polycyclic phenyl ether phosphoric acid, alkyl phosphoric acid, and salts thereof,
then adding the enzyme that catalyzes a reaction of oxidizing the glycated hemoglobin, and
reacting the glycated hemoglobin in the hemoglobin-containing sample with the enzyme that catalyzes a reaction of oxidizing the glycated hemoglobin to generate hydrogen peroxide, in the presence of the at least one anionic surfactant selected from the group consisting of N-acyl taurine in which a hydrogen atom of the amino group may be substituted with a substituent, alkyl sulfoacetic acid, polyoxyethylene alkyl ether acetic acid, N-acyl amino acid in which a hydrogen atom of the amino group may be substituted with a substituent, polyoxyethylene alkyl ether phosphoric acid, polyoxyethylene polycyclic phenyl ether phosphoric acid, alkyl phosphoric acid, and salts thereof.
7. The method according to claim 1 , wherein the at least one anionic surfactant comprises at least one surfactant selected from the group consisting of N-acyl taurine in which a hydrogen atom of the amino group is optionally substituted with a substituent, alkyl sulfoacetic acid, polyoxyethylene alkyl ether acetic acid, polyoxyethylene alkyl ether phosphoric acid, polyoxyethylene polycyclic phenyl ether phosphoric acid, alkyl phosphoric acid, and salts thereof.