CELL PREPARATION METHOD, CELL CULTIVATION DEVICE, AND KIT

1 . A method for preparing cells, the method comprising:

applying cells to a porous polyethersulfone film and culturing them.

2 . The method according to claim 1 , wherein the porous polyethersulfone film is a three-layer structure porous polyethersulfone film having a surface layer A and a surface layer B, the surface layers having a plurality of pores, and a macrovoid layer sandwiched between the surface layers A and B;

wherein an average pore diameter of the pores present in the surface layer A is smaller than an average pore diameter of the pores present in the surface layer B;

wherein the macrovoid layer has a partition wall bonded to the surface layers A and B, and a plurality of macrovoids surrounded by the partition wall and the surface layers A and B; and

wherein the pores in the surface layers A and B communicate with the macrovoids.

3 . The method according to claim 1 or 2 , the method comprising the step of:

seeding cells on the surface of the porous polyethersulfone film.

4 . The method according to claim 1 or 2 , the method comprising the steps of:

placing a cell suspension on the dried surface of the porous polyethersulfone film;

allowing the porous polyethersulfone film to stand, or moving the porous polyethersulfone film to promote efflux of liquid, or stimulating a part of the surface to cause absorption of the cell suspension into the film; and

retaining cells in the cell suspension inside the porous polyethersulfone film, and allowing water to flow out.

5 . The method according to claim 1 or 2 , the method comprising the steps of:

wetting one or both sides of the porous polyethersulfone film with a cell culture medium or a sterilized liquid;

loading a cell suspension into the wetted porous polyethersulfone film; and

retaining cells in the cell suspension inside the film, and allowing water to flow out.

6 . The method according to claim 5 , wherein living cells remain within the porous polyethersulfone film, and dead cells flows out with the water.

7 . The method according to claim 5 or 6 , wherein the sterilized liquid is a sterile water or a sterilized buffer solution.

8 . The method according to any one of claims 1 to 7 , the method comprising the step of:

placing a cell culture medium, cells, and one or more of the porous polyethersulfone films in a cell culture vessel, wherein the porous polyethersulfone films are in a suspended state in the cell culture medium.

9 . The method according to claim 8 , characterized in that two or more pieces of the porous polyethersulfone films are used.

10 . The method according to claim 8 or 9 , wherein the cells spontaneously adhere to the porous polyethersulfone film.

11 . The method according to any one of claims 1 to 7 , wherein the porous polyethersulfone film is

i) folded,

ii) wound into a roll-like shape,

iii) connected as sheets or pieces with a filamentous structure, or

iv) bound into a rope-like shape,

and suspended or fixed in a cell culture medium in a cell culture vessel.

12 . The method according to claim 11 , wherein cells spontaneously adhere to the porous polyethersulfone film.

13 . The method according to claim 1 or 2 , the method comprising using two or more porous polyethersulfone films are layered either above and below or left and right in the cell culture medium.

14 . The method according to claim 1 or 2 , wherein two or more of the methods according to any one of claims 3 to 13 are conducted in combination.

15 . The method according to any one of claims 1 to 14 , wherein cells grow and proliferate on the surface and the inside of a porous polyethersulfone film.

16 . The method according to any one of claims 1 to 15 , wherein the cells are selected from the group consisting of animal cells, insect cells, plant cells, yeasts and bacteria.

17 . The method according to claim 16 , wherein the animal cells are cells derived from an animal belonging to the vertebrate phylum.

18 . The method according to claim 16 , wherein the bacteria are selected from the group consisting of lactic acid bacteria, Escherichia coli, Bacillus subtilis and cyanobacteria.

19 . The method according to any one of claims 1 to 15 , wherein the cells are selected from the group consisting of pluripotent stem cells, tissue stem cells, somatic cells and germ cells.

20 . The method according to any one of claims 1 to 15 , wherein the cells are selected from the group consisting of sarcoma cells, established cells and transformed cells.

21 . A cell culture apparatus for use in a method for preparing cells according to any one of claims 1 to 20 , the apparatus comprising a porous polyethersulfone film.

22 . The cell culture apparatus according to claim 21 , wherein two or more porous polyethersulfone films are layered either above and below or left and right.

23 . A kit for use in a method for preparing cells according to any one of claims 1 to 20 , the kit comprising a porous polyethersulfone film.