Insecticidal proteins
Compositions and methods for controlling plant pests are disclosed. In particular, novel insecticidal proteins having toxicity Coleopteran and/or Lepidopteran insect pests are provided. Nucleic acid molecules encoding the novel insecticidal proteins are also provided. Methods of making the insecticidal proteins and methods of using the insecticidal proteins and nucleic acids encoding the insecticidal proteins of the invention, for example in transgenic plants to confer protection from insect damage, are also disclosed.
1. An expression cassette comprising a promoter operably linked to a heterologous nucleic acid molecule comprising a nucleotide sequence that encodes a protein active against a Diabrotica insect pest, wherein the nucleotide sequence:
(a) comprises any one of SEQ ID NOs: 18-37; or
(b) encodes a polypeptide, wherein the amino acid sequence of the polypeptide comprises any one of SEQ ID NOs: 2-10.
2. A nucleic acid molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 18-37.
3. A polypeptide comprising the amino acid sequence of any one of SEQ ID NOs: 2-10.
4. A vector or construct comprising the expression cassette of claim 1 .
5. A host cell comprising the expression cassette of claim 1 .
6. The host cell of claim 5 that is a bacterial cell or a plant cell.
7. A method for producing a polypeptide with insecticidal activity, said method comprising culturing the host cell of claim 5 under conditions in which the nucleic acid molecule encoding the polypeptide is expressed.
8. A method of producing a plant or plant part having enhanced resistance to a Diabrotica insect pest as compared to a control plant or plant part, said method comprising:
(a) transforming a nucleic acid molecule comprising the expression cassette of claim 1 into a plant or plant part; or
(b) crossing a first parent plant with a second parent plant, wherein at least the first parent plant comprises the expression cassette of claim 1 and producing at least one progeny plant,
wherein the transformed plant or plant part of step (a) or the at least one progeny plant of step (b) comprises the expression cassette within its genome and exhibits enhanced insect resistance as compared to a control plant or plant part.
9. The method of claim 7 , wherein the expression cassette encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 9.
10. The method of claim 8 , wherein the Diabrotica insect pest is selected from the group consisting of Diabrotica virgifera virgifera, Diabrotica barberi and Diabrotica undecimpunctata howardi.
11. The method of claim 8 , wherein the plant or plant part is a monocotyledonous plant.
12. The method of claim 11 , wherein the plant is millet, switchgrass, maize, sorghum, wheat, oat, turf grass, pasture grass, flax, rice, sugarcane, oilseed rape, or barley.
13. A transgenic plant comprising the expression cassette of claim 1 .
14. The transgenic plant of claim 13 wherein said plant is a monocotyledonous plant.
15. The transgenic plant of claim 14 , wherein said monocotyledonous plant is millet, switchgrass, maize, sorghum, wheat, oat, turf grass, pasture grass, flax, rice, sugarcane, oilseed rape, or barley.
16. The transgenic plant of claim 13 , wherein the plant comprises at least one additional desired trait, wherein the desired trait is selected from the group consisting of insect resistance, abiotic stress tolerance, male sterility, herbicide resistance, bacterial disease resistance, fungal disease resistance, viral disease resistance, nematode resistance, modified fatty acid metabolism, modified carbohydrate metabolism, production of a commercially valuable enzyme or metabolite, improved nutritional value, improved performance in an industrial process and altered reproductive capability.
17. The transgenic plant of claim 16 , wherein the additional desired trait is a second pesticidal agent that is an interfering RNA molecule.
18. A method for controlling a lepidopteran or coleopteran pest population, said method comprising contacting said population with an effective insect-controlling amount of a polypeptide encoded by the expression cassette of claim 1 .