Transcription factors to improve resistance to environmental stress in plants
Plants, plant cells, and seeds are described herein that grow well and are resistant to environmental stresses such as drought and insects, where the plants have one or more mutations that reduce or eliminate the interaction of MYC transcription factors with the JAZ proteins. The plants can have an additional mutation that reduces or eliminates the function of the PHYB gene, and/or a heterologous PIF4 transgene or PIF4 expression cassette to improve the growth of the myc mutant plants. Methods of making and using such plants, plant cells, and seeds are also described.
1. A genetically modified and non-naturally occurring mutant plant cell, a mutant plant, or a mutant plant seed obtained thereof, comprising:
(a) a PhyB loss-of-function mutation comprising at least one mutation to an amino acid in the amino acid sequence as set forth in SEO ID NO: 30; and
(b) a modified MYC nucleic acid encoding a mutant MYC protein comprising at least one mutation within a JAZ-interacting domain (JID) region of the mutant MYC protein that reduces or eliminates interaction of the mutant MYC protein with JAZ proteins, wherein the JAZ interacting domain (JID) has less than 100% and more than 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO:2, and wherein said mutant MYC protein has less than 100% amino acid sequence identity and at least 95% amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 1,
and wherein the genetically modified and non-naturally occurring mutant plant cell, said mutant plant, or said mutant plant seed obtained thereof is transgenic mutant, and wherein expression or lack of expression of said PhyB loss-of-function mutant protein, and expression of said MYC mutant protein recues wild-type plant biomass and concurrently provides JA (jasmonic acid)-controlled defense in said genetically modified and non-naturally occurring mutant plant cell, said mutant plant, or said mutant plant seed obtained thereof as compared to a control plant of the same species that is grown under identical conditions.
2. The genetically modified and non-naturally occurring mutant plant cell, said mutant plant, or said mutant plant seed obtained thereof, of claim 1 , wherein the PhyB loss-of-function mutation comprises a deletion, substitution, or insertion of a chromosomal PhyB site so that a truncated PHYB polypeptide, a mutant PHYB polypeptide, or no PHYB polypeptide is expressed.
3. The genetically modified and non-naturally occurring mutant plant cell, said mutant plant, or said mutant plant seed obtained thereof, of claim 1 , wherein the mutant MYC protein has at least one mutation in the amino acid sequence as set forth in SEQ ID NO: 1, and within the JAZ-interacting domain (JID) protein region as set forth in SEQ ID NO: 2.
4. The genetically modified and non-naturally occurring mutant plant cell, said mutant plant, or said mutant plant seed obtained thereof, of claim 1 , wherein the mutant MYC protein has reduced binding to a JAZ1 protein, and wherein the binding is reduced by at least 20% as compared to a corresponding wild type MYC protein that does not have the MYC mutation.
5. The genetically modified and non-naturally occurring mutant plant cell, said mutant plant, or said mutant plant seed obtained thereof, of claim 1 , comprising one or more mutations in one or five wild-type genomic JAZ nucleic acids that encode JAZ proteins with at least 95% amino acid sequence identity to the amino acid sequence amino acid sequence as set forth in SEQ ID NO: 48.
6. The genetically modified and non-naturally occurring mutant plant cell, said mutant plant, or said mutant plant seed obtained thereof, of claim 1 , comprising a loss-of-function chromosomal mutation in an endogenous jaz1 gene.