Method to prepare a soy hydrolysate product with a high concentration of free amino acids and method of using the same
The present development is a method for producing a high concentration free amino acid soy hydrolysate composition and a method for using the resulting hydrolysate composition. The high concentration free amino acid soy hydrolysate is used to enhance the microbe population found in soil and wastewater. The enhanced microbe population can promote plant growth, particularly for use in brownfields and similar reclamation sites or on turf grasses, and can promote the digestion of sewage solids.
1. A method of treating soil or turf grasses, the method comprising:
a. providing a potassium salt of a soy hydrolysate prepared by reacting soy meal with a diluted hydrochloric acid solution until the pH of the reaction mixture is below pH 1 and concurrently heating the reaction mixture to a temperature of at least 60° C. (140° F.) for a period of time of up to forty-eight hours, and then cooling the reaction mixture to ambient temperature and adding a base to raise the pH to at least pH 3, and then filtering the solution and recovering a free amino acid soy hydrolysate liquid composition;
b. providing soil or turf grass for treatment;
c. applying the free amino acid soy hydrolysate composition to the soil or turf grass at a concentration of from 0.1 pounds of liquid per 1000 square feet to 100 pounds of liquid per 1000 square feet;
d. allowing the hydrolysate composition to react with the soil or turf grass; and,
e. re-applying the hydrolysate composition to the soil or turf grass at predetermined intervals.
2. The method of claim 1 wherein the reaction mixture is heated to a temperature of between 100° C. (212° F.) and 105° C. (221° F.).
3. The method of claim 1 wherein the reaction mixture is heated for a period of time of from about one hour to about twenty-four hours.
4. The method of claim 1 wherein the base is selected from the group consisting of potassium hydroxide, sodium hydroxide, aqueous ammonia or a combination thereof.
5. The method of claim 1 wherein the free amino acid soy hydrolysate liquid composition contains about 30% to about 35% dissolved solids.
6. The method of claim 1 wherein the free amino acid soy hydrolysate liquid composition has about 0.5% to about 5.0% amino nitrogen, by mass.
7. The method of claim 6 wherein the free amino acid soy hydrolysate liquid composition has about 1.2% to about 2.4% amino nitrogen, by mass.
8. The method of claim 1 wherein the free amino acid soy hydrolysate has a percent dry basis aspartic acid concentration of at least 3% and a percent dry basis glutamic acid concentration of at least 4%.
9. The method of claim 1 wherein the free amino acid soy hydrolysate composition is applied by spraying a homogeneous aqueous solution of the hydrolysate composition or by spraying a heterogeneous aqueous solution of the hydrolysate composition.
10. The method of claim 1 wherein the free amino acid soy hydrolysate composition is reapplied at least every fourteen days.
11. The method of claim 1 wherein the free amino acid soy hydrolysate composition is mixed with a surfactant, an emulsifier, an antifoaming agent, a dispersant, or a combination thereof.
12. The method of claim 1 wherein the free amino acid soy hydrolysate composition is dried and applied by spreading the dried form of the hydrolysate composition.