Composition for cryopreservation of bovine reproductive cells and cryopreservation method thereof
Provided are a vehicle body assembling method and a vehicle body assembling apparatus which allow a simple configuration in the vicinity of the connecting portion between an upper jig and a lower jig and allow an increase in the efficiency of assembling work (welding work). A vehicle body assembling apparatus is equipped with a jig for supporting vehicle body components in a preassembled position, the jig comprising an upper jig and a lower jig which are connected to each other in at least two places. Each of the connection places is provided with a connecting means for fixing a three-dimensional coordinate position while allowing uniaxial turning. The vehicle body assembling apparatus is also equipped with a conveying means for conveying the upper jig which supports the vehicle body components, and reduces the load applied to the lower jig from the upper jig when connecting the upper jig to the lower jig.
1. A cryopreservation liquid with bovine sperms suspended in the cryopreservation liquid, in which bovine semen comprising the bovine sperms is diluted from 5 to 20 times, in a frozen or not-frozen state, the cryopreservation liquid comprising:
about 0.5 w/w % of an amphoteric polyelectrolyte that is antifreeze polyamino acid comprising a unit represented by formula (I) below and a unit represented by formula (II) below wherein percentage of the unit represented by the formula (II) is 50 to 99 mol %; and
about 3.25 w/w % of glycerol
2. The cryopreservation liquid with bovine sperms according to claim 1 , wherein
the cryopreservation liquid is an aqueous solution of the antifreeze polyamino acid and glycerol as dissolved in a Dulbecco's modified eagle's medium (DMEM), eagle's minimal essential medium (MEM), other cell culture solution, or in other physiological solution; and
the cryopreservation liquid is adjusted to have a pH in a range of 7.0 to 8.0 and comprises 5 to 20 w/w % of a liquid derived from bovine semen.
3. A cryopreservation liquid with bovine sperms suspended in the cryopreservation liquid, in which bovine semen comprising the bovine sperms is diluted from 5 to 20 times, in a frozen state, the cryopreservation liquid comprising:
about 0.5 w/w % of an amphoteric polyelectrolyte that is antifreeze polyamino acid comprising a unit represented by formula (I) below and a unit represented by formula (II) below:
wherein percentage of the unit represented by the formula (II) is 50 to 99 mol %; and
about 3.25 w/w % of glycerol.