Tuning CRISPR/Cas9 activity with chemically modified nucleotide substitutions
The present disclosure provides CRISPR/Cas9 ribonucleoprotein compositions comprising chemically modified CRISPR RNA (crRNA) guide and trans-acting CRISPR RNA (tracrRNA) components. Methods of using the disclosed CRISPR/Cas9 ribonucleoprotein compositions are also provided.
1. A composition comprising a Cas9 protein, a first oligonucleotide comprising a first region having a 5′ end and a 3′ end and a second region, and a second oligonucleotide comprising a first region and a second region, wherein said second region of said first oligonucleotide interacts with said first region of said second oligonucleotide, and said second region of said second oligonucleotide interacts with said Cas9 protein, wherein all nucleotides of said first oligonucleotide are 2′-deoxyribonucleotides or 2′-deoxyribonucleotide analogs.
2. The composition of claim 1 , wherein said first oligonucleotide comprises the chemical structure of crRNA_K.
3. The composition of claim 1 , wherein said first oligonucleotide comprises at least a first 2′-fluoroarabinonucleotide.
4. The composition of claim 1 , wherein said Cas9 protein comprises at least a first mutation that inactivates said Cas9 protein.
5. The composition of claim 4 , wherein said Cas9 protein comprises a D10A or a H840A mutation.
6. The composition of claim 5 , wherein said Cas9 protein comprises a D10A and a H840A mutation.
7. A method of binding a double-stranded DNA without cleaving said double-stranded DNA, comprising contacting said double-stranded DNA with the composition of claim 1 .
8. The method of claim 7 , wherein said first oligonucleotide comprises at least a first 2′-fluoroarabinonucleotide.
9. A method of inhibiting transcription of a double-stranded DNA, comprising contacting said double-stranded DNA with the composition of claim 1 .
10. The method of claim 9 , wherein said first oligonucleotide comprises at least a first 2′-fluoroarabinonucleotide.
11. The method of claim 9 , wherein said double-stranded DNA is located in a cell.
12. A method of activating transcription of a double-stranded DNA, comprising contacting said double-stranded DNA with the composition of claim 1 .
13. The method of claim 12 , wherein said first oligonucleotide comprises at least a first 2′-fluoroarabinonucleotide.
14. The method of claim 12 , wherein said double-stranded DNA is located in a cell.