Methods for diagnosing cancer
Provided herein are methods for diagnosing cancer in a subject by determining the gene expression level of one or more genes in extracellular vesicles isolated from samples obtained from a subject.
1. A method for determining the likelihood of cancer and treating the cancer in a subject in need thereof comprising:
obtaining results of levels of mRNA encoded by histone genes and E2F-target genes in extracellular vesicles isolated from a sample obtained from the subject, wherein
the histones are HIST1H3F, HIST1H3G, HIST1H2AJ, HIST1H2BM, HIST1H1B, HIST1H3B, HIST1H2AH, HIST1H3J, HIST1H2BI, HIST1H2BO and HIST1H2AI, and
the E2F targets are SPC24, CENPF, ARHGAP11B, CEP128, QPCTL, HM MR, HIST1H2BL, NDC80, KIF15, HMBS, ABCB6, HIST1H2BM, GUCY1B3, SYNGR4, RECQL4, CDCA7, TTK, CKAP2, BORA and CENPW; and
determining that the subject has an increased likelihood of having cancer when the sample from the subject comprises increased levels of the mRNAs encoded by the histone and E2F target genes relative to a reference value; and
administering an effective amount of a therapy selected from the group consisting of surgery, radiation, chemotherapy, immunotherapy, vaccine, and combinations thereof to treat the cancer to the subject having the increased likelihood of having cancer.
2. The method of claim 1 , wherein the extracellular vesicles are microvesicles, exosomes, large oncosomes or a combination thereof.
3. The method of claim 1 , wherein the sample is blood, plasma or combination thereof.
4. The method of claim 1 wherein the subject is human.
5. The method of claim 1 , wherein the reference value is the mean or median levels of the mRNA in a population of subjects that do not have cancer.
6. The method of claim 1 , wherein the reference value is the mean or median levels of the mRNA in a population of subjects that have cancer in remission.
7. The method of claim 1 , wherein the reference value is the mean or median levels of the mRNA obtained from the subject at a difference time point.