Infectious disease vaccine using non-infectious paramyxovirus particle
In this invention, a non-infectious particle has been produced, comprising a pathogen antigen protein caused to be expressed on the surface of a virus particle having at least one species of paramyxovirus envelope protein missing from the particle. This particle has been found to hold within the particle a large amount of antigen protein compared to an infectious particle, and to be capable of eliciting a host immune response with extremely high efficiency. The non-infectious particle according to the present invention is useful as a vaccine against a pathogenic virus, or the like.
1. A F gene-deleted Sendai virus non-infectious particle, which lacks F protein of the Sendai virus on the surface of the virus particle, and holds an antigen protein of a heterologous pathogen on the surface of the virus particle, wherein the antigen protein of the heterologous pathogen is a fusion protein containing an antigen fragment of the pathogen at the outer side of the virus particle and a cytoplasmic region fragment of the F protein at the inner side of the virus particle.
2. The non-infectious particle of claim 1 , wherein the antigen protein of the heterologous pathogen comprises all or part of an envelope protein of a retrovirus.
3. The non-infectious particle of claim 1 , wherein the antigen protein of the heterologous pathogen comprises all or part of an envelope protein of HTLV-1 or HIV-1.
4. A composition comprising the non-infectious particle of claim 1 .
5. A vaccine formulation comprising the non-infectious particle of claim 1 .
6. The vaccine formulation of claim 5 , which is for use in booster inoculation.
7. The vaccine formulation of claim 5 , which is used in booster inoculation, wherein the pathogen is different from a pathogen from which an antigen for primary inoculation is derived.
8. A method for producing the non-infectious particle of claim 1 , which comprises introducing into a cell a Sendai vector from which at least F protein gene has been deleted and which carries an antigen protein gene of a heterologous pathogen, and collecting a generated paramyxovirus non-infectious particle.
9. The non-infectious particle of claim 1 , wherein the antigen fragment of the pathogen is all or part of an ectodomain of an envelope protein of a pathogenic virus.
10. The non-infectious particle of claim 9 , wherein the pathogenic virus is a retrovirus.
11. The non-infectious particle of claim 9 , wherein the pathogenic virus is a T cell infectious retrovirus.
12. The non-infectious particle of claim 11 , wherein the T cell infectious virus is HTLV-1.
13. The non-infectious particle of claim 11 , wherein the T cell infectious virus is HIV-1.