Cleavable peptides and insecticidal and nematicidal proteins comprising same
A peptide comprised of either a binary or a tertiary peptide, the peptide contains at least 4 amino acids and up to a maximum of 16 amino acids, comprised of 2 or 3 different regions, wherein the binary peptides have 2 different regions and the tertiary peptides have 3 different regions; wherein, the peptide can be cleaved by both an animal gut protease and an insect or nematode gut protease.
1. A cleavable peptide linker (L) consisting of an amino acid sequence selected from the group consisting of SEQ ID NOs: 1603, 1605, 1619, 1621, 1658, 1660, 1674, 1676, 1681 and 1683, wherein the cleavable peptide linker is cleavable by a mammal gut protease, and at least one of an insect and a nematode gut protease.
2. The cleavable peptide linker (L) of claim 1 , wherein the insect gut protease is a protease derived from a Lepidoptera or a Coleoptera insect species.
3. The cleavable peptide linker (L) of claim 1 , wherein the mammal gut protease is a human gastrointestinal protease.
4. The cleavable peptide linker (L) of claim 1 , wherein the cleavable peptide linker (L) consists of the amino acid sequence as set forth in SEQ ID NO: 1603.
5. The cleavable peptide linker (L) of claim 1 , wherein the cleavable peptide linker (L)) consists of the amino acid sequence as set forth in SEQ ID NO: 1605.
6. The cleavable peptide linker (L) of claim 1 , wherein the cleavable peptide linker (L)) consists of the amino acid sequence as set forth in SEQ ID NO: 1619.
7. The cleavable peptide linker (L) of claim 1 , wherein the cleavable peptide linker (L)) consists of the amino acid sequence as set forth in SEQ ID NO: 1621.
8. The cleavable peptide linker (L) of claim 1 , wherein the cleavable peptide linker (L)) consists of the amino acid sequence as set forth in SEQ ID NO: 1658.
9. The cleavable peptide linker (L) of claim 1 , wherein the cleavable peptide linker (L)) consists of the amino acid sequence as set forth in SEQ ID NO: 1660.
10. The cleavable peptide linker (L) of claim 1 , wherein the cleavable peptide linker (L)) consists of the amino acid sequence as set forth in SEQ ID NO: 1674.
11. The cleavable peptide linker (L) of claim 1 , wherein the cleavable peptide linker (L)) consists of the amino acid sequence as set forth in SEQ ID NO: 1676.
12. The cleavable peptide linker (L) of claim 1 , wherein the cleavable peptide linker (L)) consists of the amino acid sequence as set forth in SEQ ID NO: 1681.
13. The cleavable peptide linker (L) of claim 1 , wherein the cleavable peptide linker (L)) consists of the amino acid sequence as set forth in SEQ ID NO: 1683.
14. A polynucleotide operable to encode the cleavable peptide linker (L) of claim 1 , or a complementary nucleotide sequence thereof.
15. A vector comprising a polynucleotide of claim 14 .
16. A bacterial host cell, a yeast host cell, a plant host cell or an animal host cell comprising a vector of claim 15 .