Preparation of purified collagen solution from porcine skin

1. A method of preparing a purified collagen solution from porcine skin, the method comprising:

treating the porcine skin with a sodium hydroxide treatment, resulting in a sodium hydroxide processed skin;

treating the sodium hydroxide processed skin with an ethanol treatment, resulting in ethanol treated processed skin, wherein treating the sodium hydroxide processes skin with the ethanol treatment comprises:

preparing a first ethanol soaking solution in a tank;

soaking the sodium hydroxide processed skin in the first ethanol soaking solution;

removing the sodium hydroxide processed skin from the first ethanol soaking solution and washing the sodium hydroxide processed skin;

soaking the sodium hydroxide processed skin in a second ethanol soaking solution; and

removing the sodium hydroxide processed skin from the second ethanol soaking solution;

producing liquid collagen from the ethanol treated processed skin;

clarifying the liquid collagen; and

concentrating the clarified liquid collagen,

wherein:

the first ethanol soaking solution comprises ethanol and 1,2-octanediol; and

the second ethanol soaking solution comprises ethanol without 1,2-octanediol.

2. The method of claim 1 , wherein treating the porcine skin with the sodium hydroxide treatment comprises:

preparing a soaking solution of NaOH in a tank;

soaking the porcine skin in the NaOH solution; and

removing the skin from the soaking solution.

3. The method of claim 2 , wherein:

the porcine skin is soaked in the NaOH solution at a temperature of about 4° C. for about 10 to 20 hours; and

the soaking is repeated a plurality of times.

4. The method of claim 2 , wherein the soaking solution of NaOH comprises about 20 to about 30 mL of 1M NaOH for each gram of the porcine skin.

5. The method of claim 1 , wherein:

the sodium hydroxide processed skin is soaked in the first ethanol soaking solution for about 10 to about 20 hours; and

the sodium hydroxide processed skin is soaked in the second ethanol soaking solution for about 4 to about 10 hours.

6. The method of claim 1 , wherein producing liquid collagen from the ethanol processed skin comprises:

preparing a HCl dissolving solution;

adding the dissolving solution and the ethanol processed skin to a tank; and

chilling the tank to a temperature of about 4° C.

7. The method of claim 6 , wherein:

the dissolving solution comprises about 1 L of 0.01M HCl for every 20 g of the ethanol processed skin; and

the ethanol processed skin is soaked in the dissolving solution for about 72 hours.

8. The method of claim 1 , wherein clarifying the liquid collagen comprises filtering the liquid collagen through multiple filtration steps.

9. The method of claim 8 , wherein:

a first filtration step uses filters having a size of from about 20 to about 40 μm;

a second filtration step uses filters having a size of from about 5 to about 10 μm;

a third filtration step uses filters having a size of from about 0.8 to about 8 μm; and

a fourth filtration step uses filters having a size of from about 0.22 to about 0.45 μm.

10. The method of claim 1 , wherein concentrating the clarified liquid collagen comprises using a tangential flow filtration system.