Granted Patent B2

US 10,597,690 · App. 16/356,138 · Granted Mar 24, 2020

Inventors: David N. Thompson (Idaho Falls, ID); William A. Apel (Jackson, WY); Vicki S. Thompson (Idaho Falls, ID); David W. Reed (Idaho Falls, ID); Jeffrey A. Lacey (Idaho Falls, ID); Emily D. Henriksen (Carey, NC)

Assignee: Battelle Energy Alliance, LLC

C12P19/14C12N9/1051C12N9/16C12N9/18C12N9/2402C12N9/2408C12N9/248C12N9/2428C12N9/2434C12N9/2437C12N9/2445C12N9/2465C12N9/2471C12N9/2482C12N9/2494C12N9/80C12N9/88C12N15/52C12P19/00C12P19/02C12Y204/0102C12Y301/01001C12Y301/01006C12Y301/01073C12Y301/02004C12Y302/0102C12Y302/01008C12Y302/01015C12Y302/01021C12Y302/01022C12Y302/01037C12Y302/01055C12Y302/01078C12Y302/01091C12Y302/01131C12Y302/01133C12Y302/01139C12Y305/01C12Y402/01007C12Y402/01008

Abstract

Claims (8)

1. A method of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups, the method comprising:

placing a polypeptide having at least 90% sequence identity to SEQ ID No. 253 in fluid contact with a polysaccharide, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylan, glycoside, xylan-, glucan-, galactan-, or mannan-decorating group;

wherein the polypeptide has an enzymatic activity as an esterase.

2. The method according to claim 1 , wherein placing a polypeptide having at least 90% sequence identity to SEQ ID No. 253 in fluid contact with a polysaccharide, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylan, glycoside, xylan-, glucan-, galactan-, or mannan-decorating group occurs at or below about pH 4.

3. The method according to claim 1 , wherein placing a polypeptide having at least 90% sequence identity to SEQ ID No. 253 in fluid contact with a polysaccharide, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylan, glycoside, xylan-, glucan-, galactan-, or mannan-decorating group occurs at a temperature at or above 50 degrees Celsius.

4. The method according to claim 1 , wherein the polypeptide is glycosylated, pegylated, or otherwise posttranslationally modified.

5. The method according to claim 1 , wherein the polypeptide is encoded by a nucleic acid having at least 90% identity to SEQ ID NO:252.

6. The method according to claim 1 , wherein placing a polypeptide having at least 90% sequence identity to SEQ ID NO:253 in fluid contact with a polysaccharide, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylan, glycoside, xylan-, glucan-, galactan-, or mannan-decorating group comprises translating a nucleic acid having at least 90% identity to SEQ ID NO:252 in fluid contact with the polysaccharide, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylan, glycoside, xylan-, glucan-, galactan-, or mannan-decorating group.

Assignments (2)

Continuity (8)