Carbon fixation systems in plants and algae
Provided are methods for elevating cyclic electron transfer activity, improving carbon concentration, and enhancing carbon fixation in C3 and C4 plants, and algae, and producing biomass or other products from C3 or C4 plants, and algae, selected from among, for example, starches, oils, fatty acids, lipids, cellulose or other carbohydrates, alcohols, sugars, nutraceuticals, pharmaceuticals, fragrance and flavoring compounds, and organic acids, as well as transgenic plants produced thereby. These methods and transgenic plants and algae encompass the expression, or overexpression, of various combinations of genes that improve carbon concentrating systems in plants and algae, such as bicarbonate transport proteins, carbonic anhydrase, light driven proton pump, cyclic electron flow regulators, etc.
1. A transgenic plant or alga, comprising within its genome, and expressing or overexpressing, a combination of heterologous nucleotide sequences encoding:
i) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 77 which has an activity of an ATP-dependent bicarbonate anion transporter localized to a plasma membrane, or ii) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 18 which has an activity of a bicarbonate anion transporter localized to a chloroplast envelope, or iii) a combination thereof;
iv) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 1 which has an activity of a cyclic electron transfer modulator protein, or v) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 3 which has an activity of a cyclic electron transfer modulator protein, or vi) a combination thereof; and
a protein with at least 85% amino acid sequence identity to SEQ ID NO: 21 which has an activity of a carbonic anhydrase protein.
2. The transgenic plant or alga of claim 1 wherein the bicarbonate anion transporter localized to the chloroplast envelope is an LCIA protein of SEQ ID NO: 18.
3. The transgenic plant or alga of claim 1 wherein the carbonic anhydrase is a BCA protein of SEQ ID NO: 21.
4. The transgenic plant or alga of claim 1 wherein the heterologous nucleotide sequences encode
i) SEQ ID NO: 1, and SEQ ID NO: 77; or
ii) SEQ ID NO: 1, SEQ ID NO: 77 and SEQ ID NO: 3.
5. The transgenic plant or alga of claim 2 wherein the heterologous nucleotide sequences encode SEQ ID NO: 1, and SEQ ID NO: 77.
6. The transgenic plant or alga of claim 1 wherein the heterologous nucleotide sequences encode (SEQ ID NO: 1), (SEQ ID NO: 77), (SEQ ID NO: 18), and (SEQ ID NO: 21).
7. The transgenic plant or alga of claim 1 , wherein the sequence identity is at least 90% amino acid sequence identity.
8. The transgenic plant of claim 1 , which is a C3 plant or a C4 plant.
9. The transgenic plant or alga of claim 1 further comprising a cell-part comprising within its genome, and expressing or overexpressing a combination of heterologous nucleotide sequences encoding:
i) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 77 which has an activity of an ATP-dependent bicarbonate anion transporter localized to a plasma membrane, or ii) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 18 which has an activity of a bicarbonate anion transporter localized to a chloroplast envelope, or iii) a combination thereof;
iv) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 1 which has an activity of a cyclic electron transfer modulator protein, or v) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 3 which has an activity of a cyclic electron transfer modulator protein, or vi) a combination thereof; and
a protein with at least 85% amino acid sequence identity to SEQ ID NO: 21 which has an activity of a carbonic anhydrase protein.
10. A method of making a transgenic plant or alga of claim 1 wherein said method comprises expressing, or overexpressing, in a C3 plant, a C4 plant, or an alga, a combination of heterologous nucleotide sequences encoding
i) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 77 which has an activity of an ATP-dependent bicarbonate anion transporter localized to a plasma membrane, or ii) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 18 which has an activity of a bicarbonate anion transporter localized to a chloroplast envelope, or iii) a combination thereof;
iv) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 1 which has an activity of a cyclic electron transfer modulator protein, or v) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 3 which has an activity of a cyclic electron transfer modulator protein, or vi) a combination thereof; and
a protein with at least 85% amino acid sequence identity to SEQ ID NO: 21 which has an activity of a carbonic anhydrase protein.
11. A method of elevating CET activity or of enhancing carbon fixation or for producing biomass or other products in a C3 plant, C4 plant, or alga wherein said method comprises expressing, or overexpressing, in a C3 plant, a C4 plant, or an alga, a combination of heterologous nucleotide sequences encoding:
i) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 77 which has an activity of an ATP-dependent bicarbonate anion transporter localized to a plasma membrane, or ii) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 18 which has an activity of a bicarbonate anion transporter localized to a chloroplast envelope, or iii) a combination thereof;
iv) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 1 which has an activity of a cyclic electron transfer modulator protein, or v) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 3 which has an activity of a cyclic electron transfer modulator protein, or vi) a combination thereof; and
a protein with at least 85% amino acid sequence identity to SEQ ID NO: 21 which has an activity of a carbonic anhydrase protein.
12. The method of claim 11 further comprises growing said plant or alga and harvesting said biomass or recovering said product from said plant or alga.
13. The transgenic plant or alga of claim 1 comprising within its genome, and expressing or overexpressing, a combination of heterologous nucleotide sequences encoding:
i) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 77 which has an activity of an ATP-dependent bicarbonate anion transporter localized to a plasma membrane, and ii) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 18 which has an activity of a bicarbonate anion transporter localized to a chloroplast envelope;
iv) a protein with at least 85% amino acid sequence identity to SEQ ID NO: 1 which has an activity of a cyclic electron transfer modulator protein; and
a protein with at least 85% amino acid sequence identity to SEQ ID NO: 21 which has an activity of a carbonic anhydrase protein.