Bacteriophage and methods of making and using
Bacteriophage are provided, and methods of making and using the bacteriophage also are provided.
1. A method of reducing the number of viable bacteria in tobacco comprising contacting said tobacco with an effective amount of a composition comprising a bacteriophage comprising a nucleic acid sequence at least 90% identical to SEQ ID NO: 1, wherein the reduced number of viable bacteria is as compared to a control tobacco in the absence of said composition.
2. The method of claim 1 , wherein said tobacco comprises cured tobacco.
3. The method of claim 1 , wherein said tobacco comprises reconstituted leaf tobacco.
4. The method of claim 1 , wherein said nucleic acid sequence comprises a nucleic acid sequence at least 95% identical to SEQ ID NO: 1.
5. The method of claim 1 , wherein said nucleic acid sequence comprises a nucleic acid sequence 100% identical to SEQ ID NO: 1.
6. The method of claim 1 , wherein said nucleic acid sequence encodes a polypeptide comprising an amino acid sequence at least 95% identical to SEQ ID NO: 2.
7. The method of claim 1 , wherein said nucleic acid sequence encodes a polypeptide comprising an amino acid sequence 100% identical to SEQ ID NO: 2.
8. The method of claim 1 , wherein said bacteria is from a genus selected from the group consisting of Staphylococcus and Geobacillus.
9. The method of claim 1 , wherein said method further comprises the reduction of at least one tobacco-specific nitrosamine by at leas 10% as compared to said control tobacco plant in the absence of said composition.
10. A method of reducing the number of viable bacteria in tobacco comprising contacting said tobacco with an effective amount of a composition comprising a bacteriophage comprising a nucleic acid sequence encoding an amino acid sequence comprising at least 90% sequence identity to SEQ NO: 2, wherein the reduced number of viable bacteria is as compared to a control tobacco in the absence of said composition.
11. The method of claim 10 , wherein said tobacco comprises cured tobacco.
12. The method of claim 10 , wherein said tobacco comprises reconstituted leaf tobacco.
13. The method of claim 10 , wherein said amino acid sequence comprises at least 95% sequence identity to SEQ ID NO: 2.
14. The method of claim 10 , wherein said amino acid sequence comprises 100% sequence identity to SEQ ID NO: 2.
15. The method of claim 10 , wherein said nucleic acid sequence comprises at least 95% sequence identity to SEQ ID NO: 1.
16. The method of claim 10 , wherein said nucleic acid sequence comprises 100% identity to SEQ ID NO: 1.
17. The method of claim 10 , wherein said bacteria is from a genus selected from the group consisting of Staphylococcus and Geobacillus.
18. The method of claim 10 , wherein said method further comprises the reduction of at least one tobacco-specific nitrosamine by at least 10% as compared to said control tobacco plant in the absence of said composition.
19. A method of reducing at least one tobacco-specific nitrosamine (TSNA) in tobacco comprising contacting said tobacco with an effective amount of a composition comprising a bacteriophage comprising a nucleic acid sequence encoding an amino acid sequence comprising at least 90% sequence identity to SEQ ID NO: 2, wherein the reducing at least one TSNA is as compared to a control tobacco in the absence of said composition.
20. The method of claim 19 , wherein the at least one TSNA is reduced by at least 10%.