Complementary color flashing for multichannel image presentation
Methods are provided for the highlighting of features in composite images through the alternating of images having complementary colors. An image having a feature of interest is used to generate one or more pseudo color images. A series of a pseudo color images and one or more additional pseudo color or original color images are then alternately displayed so that the differently colored regions among the series of images are easily recognizable to an operator. The differently colored regions differ in having hues that are complementary to one another. The methods are particularly useful for the display of information using two or more imaging modalities and channels, such as is the case for some medical applications in which a natural-light image of pink or light-red tissue with deeper red or blue vasculature is overlaid with another functional image. In these cases, a feature present in the functional image can be more easily perceived when displayed in a composite overlay with an underlying image from another imaging modality or channel.
1. A method of displaying images of a biological sample, the method comprising:
receiving a first image representing a view from a viewpoint;
selecting an original color from the first image, wherein the original color has a first hue;
generating a second image from the first image by replacing the selected original color of the first image with a false color having a hue on an opposite side of a hue/saturation/value color space from the first hue; and
alternately displaying the first and second images.
2. The method of claim 1 , wherein the first image has a minimum first image color value and a maximum first image color value, and wherein the false color has a false color value that is within the range between the minimum and maximum first image color values.
3. The method of claim 1 , wherein the alternately displaying is performed at a frequency.
4. The method of claim 3 , wherein the frequency of the alternately displaying is between 0.1 Hz and 25 Hz.
5. The method of claim 3 , further comprising:
accepting operator input selecting the frequency of the alternately displaying.
6. The method of claim 1 , wherein the first image is a reflected light image, a fluorescence image, an X-ray image, a positron emission tomography (PET) image, a photon emission computed tomography (SPECT) image, a magnetic resonance imaging (MRI) image, a nuclear magnetic resonance (NMR) image, an ultrasound image, an autoradiography image, an immunohistochemistry image, or a microscopy image.
7. The method of claim 1 , further comprising:
accepting operator input selecting the viewpoint.
8. A machine-readable non-transitory medium embodying information indicative of instructions for causing a computer processor to perform operations for displaying images, the operations comprising:
receiving a first image representing a view from a viewpoint;
selecting an original color from the first image, wherein the original color has a first hue;
generating a second image from the first image by replacing the selected original color of the first image with a false color having a hue on an opposite side of a hue/saturation/value space from the first hue; and
alternately displaying the first and second images.
9. The machine-readable non-transitory medium of claim 8 , wherein the first image has a minimum first image color value and a maximum first image color value, and wherein the false color has a false color value that is within the range between the minimum and maximum first image color values.
10. The machine-readable non-transitory medium of claim 8 , wherein the alternately displaying is performed at a frequency.
11. The machine-readable non-transitory medium of claim 10 , wherein the frequency of the alternately displaying is between 0.1 Hz and 25 Hz.
12. The machine-readable non-transitory medium of claim 10 , wherein the operations further comprise:
accepting operator input selecting the frequency of the alternately displaying.
13. The machine-readable non-transitory medium of claim 8 , wherein the operations further comprise:
accepting operator input selecting the viewpoint.
14. A computer system for displaying images, the system comprising:
at least one processor, and
a memory operatively coupled with the at least one processor, the at least one processor executing instructions from the memory comprising:
program code for receiving a first image representing a view from a viewpoint;
program code for selecting an original color from the first image, wherein the original color has a first hue;
program code for generating a second image from the first image by replacing the selected original color of the first image with a false color having a hue on an opposite side of a hue/saturation/value color space from the first hue; and
program code for alternately displaying the first and second images.
15. The computer system of claim 14 , wherein the first image has a minimum first image color value and a maximum first image color value, and wherein the false color has a false color value that is within the range between the minimum and maximum first image color values.
16. The computer system of claim 14 , wherein the alternately displaying is performed at a frequency.
17. The computer system of claim 16 , wherein the frequency of the alternately displaying is between 0.1 Hz and 25 Hz.
18. The computer system of claim 16 , wherein the instructions executed by the at least one processor further comprise:
accepting operator input selecting the frequency of the alternately displaying.
19. The computer system of claim 14 , wherein the instructions executed by the at least one processor further comprise:
accepting operator input selecting the viewpoint.