Compositions and methods for altering flowering and plant architecture to improve yield potential
The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.
1. A transgenic soybean plant comprising an insertion of a recombinant DNA construct comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a meristem-preferred or meristem-specific promoter comprising a polynucleotide sequence that is at least 90% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 21, SEQ ID NO: 22, and SEQ ID NO: 38, wherein the florigenic FT protein comprises an amino acid sequence having at least 92% identity to a sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 8, and SEQ ID NO: 12 into the genome of the transgenic soybean plant, wherein the transgenic soybean plant has more floral racemes per node than a control plant not having the recombinant DNA construct.
2. The transgenic soybean plant of claim 1 , wherein the transgenic soybean plant is homozygous for the insertion of the recombinant DNA construct.
3. The transgenic soybean plant of claim 1 , wherein the transgenic soybean plant is hemizygous for the insertion of the recombinant DNA construct.
4. The transgenic soybean plant of claim 1 , wherein the transgenic soybean plant produces more flowers per node than a control plant not having the recombinant DNA construct.
5. The transgenic soybean plant of claim 1 , wherein the transgenic soybean plant flowers earlier than a control plant not having the recombinant DNA construct.
6. The transgenic soybean plant of claim 1 , wherein the transgenic soybean plant produces more pods per node than a control plant not having the recombinant DNA construct.
7. The transgenic soybean plant of claim 1 , wherein the polynucleotide sequence encoding the florigenic FT protein has at least 90% identity to a sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 7, SEQ ID NO: 9, and SEQ ID NO: 11.
8. A transgenic soybean plant part of the transgenic soybean plant of claim 1 , wherein the transgenic plant part is one of the following: a seed, fruit, leaf, cotyledon, hypocotyl, meristem, embryo, endosperm, root, shoot, stem, pod, flower, inflorescence, stalk, pedicel, style, stigma, receptacle, petal, sepal, pollen, anther, filament, ovary, ovule, pericarp, phloem, or vascular tissue.
9. The transgenic soybean plant of claim 1 , wherein the florigenic FT protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 8, and SEQ ID NO: 12.
10. The transgenic soybean plant of claim 1 , wherein the promoter comprises SEQ ID NO: 21, SEQ ID NO: 22, or SEQ ID NO: 28.
11. The transgenic soybean plant of claim 1 , wherein the florigenic FT protein comprises an amino acid sequence having at least 95% identity to a sequence selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 8, and SEQ ID NO: 12.
12. The transgenic soybean plant of claim 1 , wherein the florigenic FT protein further comprises one or more of the following amino acids: a tyrosine or other uncharged polar or nonpolar residue at the amino acid position of the florigenic FT protein corresponding to amino acid position 85 of SEQ ID NO: 14; a leucine or other nonpolar residue at the amino acid position of the florigenic FT protein corresponding to amino acid position 128 of SEQ ID NO: 14; and a tryptophan or other large nonpolar residue at the amino acid position of the florigenic FT protein corresponding to amino acid position 138 of SEQ ID NO: 14.
13. The transgenic soybean plant of claim 1 , wherein the florigenic FT protein does not have one or more of the following amino acids: a histidine at the amino acid position corresponding to a lysine or arginine at the amino acid position corresponding to position 85 of SEQ ID NO: 14; a lysine or arginine at the amino acid position corresponding to position 128 of SEQ ID NO: 14; and a serine, aspartic acid, glutamic acid, lysine or arginine at the amino acid position corresponding to position 138 of SEQ ID NO: 14.