COMPOSITIONS AND METHODS FOR PROVIDING HEMATOPOIETIC FUNCTION WITHOUT HLA MATCHING
The present invention relates to methods and compositions for providing hematopoietic function in immunodeficient human patients, by selecting an expanded human umbilical cord blood stem/progenitor cell sample without taking into account the HLA-type of the expanded human cord blood stem/progenitor sample or the HLA-type of the patient; and administering the selected expanded human cord blood stem/progenitor cell sample to the patient. Methods for obtaining the expanded human cord blood stem/progenitor cell samples, banks of frozen expanded human cord blood stem/progenitor cell samples, and methods for producing such banks are also provided herein.
1 . A method of treating acute myelogenous leukemia (AML) in a human patient, comprising:
administering to the human patient a CD34+ enriched, T cell depleted, expanded human cord blood stem and progenitor cell product, wherein the cell product is derived from samples from at least two human donors without regard to the HLA type of the donors and without taking into account the HLA type of the human patient; and
allowing transient engraftment of cells from the cell product;
thereby treating AML in the human patient.
2 . The method of claim 1 , wherein the cell product was produced by steps comprising enriching for CD34+ human cord blood stem and progenitor cells; and expanding the CD34+ enriched human cord blood stem and progenitor cells with a Notch agonist.
3 . The method of claim 1 , wherein the human patient had been previously treated with an intensive chemotherapy regimen or a myeloablative regimen for hematopoietic cell transplantation.
4 . The method of claim 1 , wherein the cell product comprises at least 75 million viable CD34+ cells.
5 . The method of claim 4 , wherein the cell product comprises at least 250 million viable CD34+ cells.
6 . The method of claim 1 , wherein the human patient has an immunodeficiency.
7 . The method of claim 1 , wherein the cell product further comprises a cryoprotective agent.
8 . The method of claim 1 , wherein the cell product has been thawed.