Method for producing a dairy product
The present invention relates to a method for producing a dairy product using an enzyme having lactase activity.
1. A polypeptide having transgalactosylating activity, which is a truncated polypeptide consisting of the amino acid sequence of amino acids 28-979 of SEQ ID NO: 1 or is a fragment thereof having transgalactosylating activity, wherein the polypeptide is isolated.
2. The polypeptide of claim 1 , wherein the truncated polypeptide consists of the amino acid sequence of amino acids 28-979 of SEQ ID NO: 1.
3. The polypeptide of claim 1 , wherein the truncated polypeptide consists of the amino acid sequence of a fragment of amino acids 28-979 of SEQ ID NO: 1.
4. A composition comprising the polypeptide of claim 1 .
5. The composition of claim 4 , which is a food composition.
6. The composition of claim 5 , which is a dairy product.
7. A method for producing a food product, comprising treating a substrate comprising lactose with the polypeptide of claim 1 .
8. A method for producing a dairy product, comprising treating a milk-based substrate comprising lactose with the polypeptide of claim 1 .
9. A method for producing galacto-oligosaccharides, comprising contacting the polypeptide of claim 1 with a milk-based substrate comprising lactose.
10. A method for producing galacto-oligosaccharides, comprising contacting the polypeptide of claim 2 with a milk-based substrate comprising lactose.
11. A polypeptide having transgalactosylating activity, which is a fragment having an amino acid sequence which is at least 98% identical to amino acids 28-979 of SEQ ID NO: 1, wherein the fragment consists of at most 952 amino acid residues and has transgalactosylating activity, wherein the polypeptide is isolated.
12. A composition comprising the polypeptide of claim 11 .
13. The composition of claim 12 , which is a food composition.
14. The composition of claim 13 , which is a dairy product.
15. A method for producing a food product, comprising treating a substrate comprising lactose with the polypeptide of claim 11 .
16. A method for producing a dairy product, comprising treating a milk-based substrate comprising lactose with the polypeptide of claim 11 .
17. A method for producing galacto-oligosaccharides, comprising contacting the polypeptide of claim 11 with a milk-based substrate comprising lactose.