Anti-human Igβ antibody
Described herein are novel anti-human Igβ antibodies, as well as methods for making the antibodies and using the antibodies to treat or prevent autoimmune disease.
1. A polynucleotide encoding a heavy chain of an anti-human Igβ antibody of any one of the following (a) to (c); or encoding a light chain of an anti-human Igβ antibody of any one of the following (a) to (c):
(a) an anti-human Igβ antibody comprising a heavy chain and a light chain, wherein the heavy chain comprises a heavy chain variable region consisting of the amino acid sequence of amino acid numbers 1 to 119 of SEQ ID NO: 6 and a heavy chain constant region which is a human Igγ1 constant region having amino acid mutations of S239D, H268D, and L328W, and the light chain comprises a light chain variable region consisting of the amino acid sequence of amino acid numbers 1 to 112 of SEQ ID NO: 8;
(b) an anti-human Igβ antibody comprising a heavy chain and a light chain, wherein the heavy chain comprises a heavy chain variable region consisting of the amino acid sequence of amino acid numbers 1 to 119 of SEQ ID NO: 2 and a heavy chain constant region which is a human Igγ1 constant region having amino acid mutations of S239D, H268D, and L328W, and the light chain comprises a light chain variable region consisting of the amino acid sequence of amino acid numbers 1 to 112 of SEQ ID NO: 4;
(c) an anti-human Igβ antibody comprising a heavy chain and a light chain, wherein the heavy chain comprises a heavy chain variable region consisting of the amino acid sequence of amino acid numbers 1 to 119 of SEQ ID NO: 10 and a heavy chain constant region which is a human Igγ1 constant region having amino acid mutations of S239D, H268D, and L328W, and the light chain comprises a light chain variable region consisting of the amino acid sequence of amino acid numbers 1 to 112 of SEQ ID NO: 12.
2. An expression vector comprising the polynucleotide of claim 1 .