IP Library › Granted Patent US 10,836,798
Granted Patent B2
US 10,836,798 · App. 16/395,407 · Granted Nov 17, 2020

Amino acid-specific binder and selectively identifying an amino acid

Inventors: Jennifer A. Tullman (Germantown, MD); Zvi Kelman (Gaithersburg, MD); John P. Marino (Silver Spring, MD)
Assignee: GOVERNMENT OF THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF COMMERCE
C07K14/195C07K1/13G01N33/582
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Quick Facts
Patent No.
US 10,836,798
App. No.
16/395,407
Granted
Nov 17, 2020
Kind
B2
Abstract

An amino acid-specific binder selectively binds to a binding amino acid. A binder complex selectively identifies the binding amino acid and includes an adjunct attached to the amino acid-specific binder. The adjunct includes a taggant, protein, substrate, or chemical modifier. Selectively identifying an N-terminal amino acid includes anchoring a C-terminal end; contacting an N-terminal amino acid of the anchored analyte with the binder complex; selectively binding when the N-terminal amino acid includes the binding amino acid; producing, by the taggant of the tagged complex, a taggant signal; detecting the taggant signal; and identifying the N-terminal amino acid based on the taggant signal.

Claims (71)

1. An amino acid-specific binder for selectively binding to an amino acid in an analyte, the amino acid-specific binder comprising:

a first amino acid sequence comprising

(Sequence ID No. 1)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTCPSFVTVVLKAVFRMSE

DTGRRVMMTAHRFGSAVVVVCERDIAETKAKEATDLGKEAGFPLMFTTEP

EE;

a second amino acid sequence comprising

(Sequence ID No. 2) 

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTCSWFVTVVLKAVFRMSE

DTGRRVMMTAHRFGSAVVVVCERDIAETKAKEATDLGKEAGFPLMFTTEP

EE;

a third amino acid sequence comprising

(Sequence ID No. 3)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPMSFVTVVLKAVFRMSE

DTGRRVMMTAHRFGSAVVVVCERDIAETKAKEATDLGKEAGFPLMFTTEP

EE;

a fourth amino acid sequence comprising

(Sequence ID No. 4)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTSGRFVTVVLKAVFRMSE

DTGRRVMMTAHRFGSAVVVVCERDIAETKAKEATDLGKEAGFPLMFTTEP

EE;

a fifth amino acid sequence comprising

(Sequence ID No. 5)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPMPFVTVVLKAVFRMSE

DTGRRVMMTAHRFGSAVVVVCERDIAETKAKEATDLGKEAGFPLMFTTEP

EE;

a sixth amino acid sequence comprising

(Sequence ID No. 6)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPREFVTVVLKAVFRMSE

DTGRRVMMTAHRFGSAVVVVSERDIAETKAKEATDLGKEAGFPLMFTTEP

EE;

a seventh amino acid sequence comprising

(Sequence ID No. 7)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPREFVTEVLKAVFNMSE

DQGRRVMMTAHRFGSAVVGVCTRDIAETKAKQATDLAREAGFPLMFTTEP

EE;

an eighth amino acid sequence comprising

(Sequence ID No. 8)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPMSFVTEVLKAVFNMSE

DQGRRVMMTAHRFGSAVVGVSTRDIAETKAKQATDLAREAGFPLMFTTEP

EE;

or

a ninth amino acid sequence comprising

(Sequence ID No. 10)

NLEKIKKLRNVIKEIKKDNIKEADEHEKKEREKETSAWKVILYNDDIHKF

SYVTDVIVKVVGQISKAKAHTITVEAHSTGQALILSTWKSKAEKYCQELQ

QNGLTVSIIHESQLKDKQKK.

2. An amino acid-specific binder for selectively binding to an amino acid in an analyte, the amino acid-specific binder comprising the amino acid sequence comprising:

a first amino acid sequence comprising X1-C-P-S-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8 (Sequence ID No. 1);

a second amino acid sequence comprising X1-C-S-W-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8 (Sequence ID No. 2);

a third amino acid sequence comprising X1-P-M-S-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8 (Sequence ID No. 3);

a fourth amino acid sequence comprising X1-S-G-R-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8 (Sequence ID No. 4);

a fifth amino acid sequence comprising X1-P-M-P-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8 (Sequence ID No. 5);

a sixth amino acid sequence comprising X1-P-R-E-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8 (Sequence ID No. 6);

a seventh amino acid sequence comprising X1-P-R-E-X2-V-X3-R-X4-T-X5-S-E-X6-E-X7-G-K-X8 (Sequence ID No. 7);

an eighth amino acid sequence comprising X1-P-M-S-X2-E-X3-N-X4-Q-X5-S-T-X6-Q-X7-A-R-X8 (Sequence ID No. 8);

wherein:

X1 comprises the amino acid sequence comprising SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYT (Sequence ID No. 20);

X2 comprises the amino acid sequence comprising FVT;

X3 comprises the amino acid sequence comprising VLKAVF (Sequence ID No. 22);

X4 comprises the amino acid sequence comprising MSED (Sequence ID No. 23);

X5 comprises the amino acid sequence comprising GRRVMMTAHRFGSAVVVV (Sequence ID No. 24);

X6 comprises the amino acid sequence comprising RDIAETKAK (Sequence ID No. 25);

X7 comprises the amino acid sequence comprising ATDL (Sequence ID No. 26); and

X8 comprises the amino acid sequence comprising EAGFPLMFTTEPEE (Sequence ID No. 27),

such that a total percentage amount of substitutions and deletions to X1, X2, X3, X4, X5, X6, X7, and X8 is from 0% to less than 30%,

exclusive of

(Sequence ID No. 27)

SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTCPSFVTVVLKAVFRMSE

DTGRRVMMTAHRFGSAVVVVCERDIAETKAKEATDLGKEAGFPLMFTTEP

EE.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 10, 2019
From: TULLMAN, JENNIFER A.; MARINO, JOHN P.; KELMAN, ZVI
To: GOVERNMENT OF THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF COMMERCE
Reel/Frame 049419/0026 →
Continuity (2)
Provisional Application 62757271 · Nov 8, 2018
Related Publication 20200148727A1 · May 14, 2020