Reaction treatment container and reaction treatment device
A reaction processing vessel includes: a substrate; a channel for a sample to move that is formed on the substrate; a first air communication port and a second air communication port provided at respective ends of the channel; and a thermal cycle region for applying a thermal cycle to the sample that is formed between the first air communication port and the second air communication port in the channel. The channel includes a first branch channel and a second branch channel between the thermal cycle region and the first air communication port.
1. A reaction processor for causing PCR in a sample comprising:
a reaction processing vessel comprising:
a substrate;
a channel for a sample to move that is provided on the substrate, the channel including a first serpiginous portion and a second serpiginous portion;
a first air communication port and a second air communication port provided at both ends of the channel for blowing air into the channel; and
a sample introduction port for introducing the sample into the channel provided between the first air communication port and the second air communication port;
wherein the first air communication port, the first serpiginous portion, the second serpiginous portion and the second air communication port are provided in sequence, and
wherein the channel includes two branch channels stemming from a portion near the first air communication port into the first serpiginous portion;
a reaction processing vessel placing portion;
a first heater and a second heater in contact with the reaction processing vessel, the first heater maintaining the first serpiginous portion at a first temperature to provide a first temperature region and the second heater maintaining the second serpiginous portion at a second temperature lower than the first temperature to provide a second temperature region; and
a liquid feeding system including a micro blower pump, connecting to the first air communication port and the second air communication port, moving the sample reciprocally between the first temperature region and the second temperature region by alternately blowing air from the first air communication port and second air communication port.
2. The reaction processor according to claim 1 ,
further comprising a fluorescence detector configured to detect fluorescence from the sample in the channel between the first serpiginous portion and the second serpiginous portion.
3. The reaction processor according to claim 1 ,
wherein the liquid feeding system includes a first micro blower pump connecting to the first air communication port and a second micro blower pump connecting to the second air communication port,
wherein the first micro blower pump and the second micro blower pump alternately operate and stop to blow into the channel alternately from the first air communication port and the second air communication port, to move the sample between the first serpiginous portion and the second serpiginous portion reciprocately.
4. The reaction processor according to claim 2 ,
wherein the liquid feeding system includes a first micro blower pump connecting to the first air communication port and a second micro blower pump connecting to the second air communication port,
wherein the first micro blower pump and the second micro blower pump alternately operate and stop to blow into the channel alternately from the first air communication port and the second air communication port, to move the sample between the first serpiginous portion and the second serpiginous portion reciprocately.
5. The reaction processor according to claim 4 ,
wherein the first micro blower pump and the second micro blower pump alternately operate and stop based on changes in fluorescence detected by the fluorescence detector.
6. The reaction processor according to claim 1 ,
wherein a liquid mass not subjected to PCR blocks one of the two branch channels.
7. The reaction processor according to claim 6 ,
wherein the liquid mass comes from a condensation of a portion of an evaporated sample.
8. The reaction processor according to claim 1 , further comprising;
a third serpiginous portion provided between the second air communication port and the second serpiginous portion, the third serpiginous portion having the sample introduction port for introducing the sample into the channel; and
a third heater maintaining the sample introduction port at a third temperature lower than the second temperature.
9. The reaction processor according to claim 1 , further comprising;
a first filter provided between the first air communication port and the two branch channels and a second filter provided between the second air communication port and the second serpiginous region.