Breath Capture and Analysis System
Systems and methods directed to aldehyde detection are disclosed. An aldehyde detection system may capture aldehydes from a patient breath sample. Aldehydes of the breath sample may be used to form a mobile chromatography phase within an analysis device. The analysis device may include various modules configured to perform a high pressure liquid chromatography process that separates aldehydes according to size. A detection assembly may detect a relative value of the separated aldehydes. The analysis device may be configured to determine an aldehyde score or metric based on the detected aldehydes, which may assist in the diagnosis of certain medical conditions.
1 . A breath analysis system, comprising:
a breath capture component having an internal volume;
a cartridge attachable to the breath capture component and having a permeable membrane;
an analysis device having a column, a first reagent and a second reagent, the analysis device coupled with the cartridge; and
wherein the analysis device is configured to:
draw a breath sample held within the internal volume of the breath capture device through the permeable membrane;
elute the breath sample from the permeable membrane using the first reagent;
input the eluted breath sample to the column using the second reagent; and
detect one or more target compounds in the breath sample at an output of the column.
2 . The breath analysis system of claim 1 , wherein:
the breath capture component is an inflatable bag configured to receive the breath sample from a user.
3 . The breath analysis system of claim 1 , wherein:
the permeable membrane is composed of a bed of silica.
4 . The breath analysis system of claim 1 , wherein:
the column is composed of a high density silica configured to separate the target compounds in the breath sample based on the target compound's molecular weight.
5 . The breath analysis system of claim 4 , wherein:
the target compounds in the breath sample are target compounds having one or more carbonyl containing moieties.
6 . The breath analysis system of claim 5 , wherein:
the target compounds having one or more carbonyl containing moieties are aldehydes, ketones, carboxylic acids, or mixtures thereof.
7 . The breath analysis system of claim 6 , wherein:
the aldehydes are aliphatic aldehydes, di-aldehydes, aromatic aldehydes, or mixtures thereof.
8 . The breath analysis system of claim 4 , wherein:
the analysis device further comprises an optical detector;
the second reagent is a dye attachable to the one or more target compounds; and
the optical detector detects a value of the separated target compounds by measuring a fluorescence of the dye when hit by an excitation source.
9 . The breath analysis system of claim 8 , further comprising:
a display configured to depict a graphical output corresponding to the value.
10 . A method for determining a target compound content in a breath sample, comprising:
drawing a breath sample through a permeable membrane connected to an analysis device;
eluting the breath sample from the permeable membrane using a first reagent from a container positioned within the analysis device;
advancing the eluted breath sample through a column using a second reagent from the container; and
detecting the target compound in the breath sample at an output of the column corresponding to the target compound content of the breath sample;
wherein the container comprises a quantity of the first reagent and the second reagent sufficient to determine the target compound content in the breath sample.
11 . The method of claim 10 , further comprising:
after the eluting and before the advancing, mixing the eluted breath sample with a fluorescent dye, wherein the fluorescent dye is configured to attach to the target compound in the breath sample to form fluoresced particles.
12 . The method of claim 11 , wherein the detecting further comprises:
propagating a laser through the output of the column; and
detecting the fluoresced particles by measuring an increase in brightness of the fluorescent dye.
13 . The method of claim 12 , further comprising:
blocking spectrum wavelength associated with the laser from reaching a detector used to measure the increase in brightness of the dye.
14 . The method of claim 11 , further comprising:
after the optical detector detects the value of the separated target compounds by measuring the fluorescence of the dye when hit by the excitation source;
drawing a second breath sample through the permeable membrane connected to the analysis device;
eluting the second breath sample from the permeable membrane using the first reagent from the container positioned within the analysis device:
advancing the eluted second breath sample through the column using the second reagent from the container; and
detecting the target compounds in the second breath sample at the output of the column corresponding to the target compound content of the second breath sample;
wherein the container comprises a quantity of the first reagent and the second reagent sufficient to determine the target compound content in the second breath sample.
15 . An analysis device, comprising:
a sample capture module configured to retain target compounds from a breath sample;
a mixing module coupled to the sample capture module and configured to mix the retained target compounds with a group of first reagents;
an injection module separated from the mixing module and configured to form a pressurized combination of one or more second reagents and a buffer; and
a detection module configured to determine a value of the retained target compounds by:
receiving an output of the mixing module in a first configuration that loads a sample loop;
in response to loading a volume of the sample loop, receiving an output of the injection module in a second configuration that advances the loaded volume through a column; and
detecting a value for the retained target compounds.
16 . The analysis device of claim 15 , wherein:
the sample capture module is configured to form an elution having the retained target compounds; and
in response to a detection of the elution, the mixing module is configured to initiate the mixing of the second reagents and buffer with the elution having the retained target compounds.
17 . The analysis device of claim 15 , wherein, the injection module is configured to form the pressurized combination by combining the second reagent having a first flow rate with the buffer having a second flow rate into a common flow path.
18 . The analysis device of claim 16 , wherein:
the injection module is configured to detect a depressurization of the pressurized combination of the second reagent and the buffer; and
in response to the detection of the depressurization, the detection module is configured to initiate a third configuration that advances an output of the injection module to a waste outlet.
19 . The analysis device of claim 16 , wherein the one or more second reagents includes a fluorescent dye configured to react with the target compounds in the elution to form dye particles.
20 . The analysis device of claim 19 , wherein the detection module further comprises a laser and an optical detector, such that:
the laser exposes an output of the column to radiation that causes the dye particles to fluoresce and the detecting to be with the optical detector.