Pharmaceutical composition for controlled release of treprostinil
Provided herein are pharmaceutical compositions containing (a) at least one liposome includes at least one vesicle-forming phospholipid; and (b) treprostinil encapsulated within the liposome. The ratio of treprostinil to phospholipid is equal to or higher than 0.035 and provides a controlled release of treprostinil. Also provided is the use of the pharmaceutical compositions to treat respiratory diseases.
1. A pharmaceutical composition, comprising:
one or more liposome suspended in an external medium, said liposome comprising:
(a) an external lipid bilayer, comprising at least one vesicle-forming phospholipid, wherein the vesicle-forming lipid consists of a mixture of a first phospholipid selected from the group consisting of phosphatidylcholine (PC), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidic acid (PA), phosphatidyethanolamine (PE), phosphatidylserine (PS) and any combination thereof and a second phospholipid selected from the group consisting of a PEG modified phospholipid, a positively charged or a negatively charged phospholipid, a charged lipid and any combination thereof; and
(b) an internal aqueous medium, comprising treprostinil and a salt to provide a pH gradient between the internal aqueous medium and the external medium, said salt is a weak acid salt or an amino acid,
wherein the weight ratio of treprostinil to the at least one vesicle-forming phospholipid is equal to or higher than about 0.035 and about less than 60% of the treprostinil is released within 2 hours after the administration of the pharmaceutical composition and more than 80% of the treprostinil is released more than 2 hours to about 72 hours after the administration of the pharmaceutical composition.
2. The pharmaceutical composition of claim 1 , wherein the weight ratio of treprostinil to the at least one vesicle-forming phospholipid equal to or higher than about 0.041.
3. The pharmaceutical composition of claim 1 , wherein the weight ratio of treprostinil to the at least one vesicle-forming phospholipid is equal to or higher than about 0.052.
4. The pharmaceutical composition of claim 1 , wherein the weight ratio of treprostinil to the at least one vesicle-forming phospholipid is equal to or higher than about 0.056.
5. The pharmaceutical composition of claim 1 , wherein the external lipid bilayer further comprising a sterol.
6. The pharmaceutical composition of claim 5 , wherein the sterol is selected from the group consisting of cholesterol, cholesterol hexasuccinate, ergosterol, lanosterol, and any combination thereof.
7. The pharmaceutical composition of claim 1 , wherein the weak acid salt is carboxylic acid salt or bicarbonate salt.
8. The pharmaceutical composition of claim 7 , wherein the carboxylic acid salt is selected from the group consisting of formate, acetate, propionate, butyrate, isobutyrate, valerate, isovalerate, benzoate and any combination thereof.
9. The pharmaceutical composition of claim 8 , wherein the acetate is sodium acetate, calcium acetate or any combination thereof.
10. The pharmaceutical composition of claim 1 , wherein the amino acid is a polar amino acid.
11. The pharmaceutical composition of claim 10 , wherein the polar amino acid is a neutral polar amino acid.
12. The pharmaceutical composition of claim 10 , wherein the polar amino acid is a basic polar amino acid.
13. The pharmaceutical composition of claim 1 , wherein more than 80% of the treprostinil is released more than 2 hours to about 48 hours after the administration of the pharmaceutical composition.
14. The pharmaceutical composition of claim 1 , wherein more than 80% of the treprostinil is released more than 2 hours to 24 hours after the administration of the pharmaceutical composition.
15. The pharmaceutical composition of claim 1 , wherein the internal aqueous medium is substantially free of precipitation.
16. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition is substantially free of a detergent or an ionophore.
17. The pharmaceutical composition of claim 1 , wherein the first phospholipid is selected from HSPC, DSPC, DPPC, DMPC or any combination thereof and the second phospholipid selected from DSPG, DPPG, DMPG, PEG-DSPE, or any combination thereof.
18. The pharmaceutical composition of claim 1 , wherein the first phospholipid is selected from HSPC, DSPC, DPPC, DMPC or any combination thereof and the charged lipid is stearylamine, 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), 3ß-[N—(N′,N′-dimethylaminoethane)-carbamoyl]cholesterol (DC-Cholesterol), N 4 -Cholesteryl-Spermine (GL67), dimethyldioctadecylammonium (DDAB), 1,2-di-O-octadecenyl-3-trimethylammonium propane (DOTMA), ethylphosphocholine (ethyl PC) or any combination thereof.