Methods and compositions for increasing red blood cells
In certain aspects, the present invention provides compositions and methods for increasing red blood cell and/or hemoglobin levels in vertebrates, including rodents and primates, and particularly in humans.
1. A method for treating anemia in a patient, the method comprising administering to a patient in need thereof a polynucleotide encoding a BMP9 and/or a BMP10 polypeptide; wherein the BMP9 and/or BMP10 polypeptide binds to ALK1 and increases red blood cell levels in the patient.
2. The method of claim 1 , wherein the nucleotide encodes a BMP9 polypeptide, and wherein the BMP9 polypeptide comprises an amino acid sequence that is at least 80% identical to the sequence of SEQ ID NO: 3.
3. The method of claim 1 , wherein the nucleotide encodes a BMP9 polypeptide, and wherein the BMP9 polypeptide comprises an amino acid sequence that is at least 90% identical to the sequence of SEQ ID NO: 3.
4. The method of claim 1 , wherein the nucleotide encodes a BMP9 polypeptide, and wherein the BMP9 polypeptide comprises an amino acid sequence that is at least 95% identical to the sequence of SEQ ID NO: 3.
5. The method of claim 1 , wherein the BMP9 polypeptide comprises an amino acid sequence that is identical to the sequence of SEQ ID NO: 3.
6. The method of claim 1 , wherein the BMP10 polypeptide comprises an amino acid sequence that is at least 80% identical to the sequence of SEQ ID NO: 6.
7. The method of claim 1 , wherein the nucleotide encodes a BMP10 polypeptide, and wherein the BMP10 polypeptide comprises an amino acid sequence that is at least 90% identical to the sequence of SEQ ID NO: 6.
8. The method of claim 1 , wherein the nucleotide encodes a BMP10 polypeptide, and wherein the BMP10 polypeptide comprises an amino acid sequence that is at least 95% identical to the sequence of SEQ ID NO: 6.
9. The method of claim 1 , wherein the BMP10 polypeptide comprises an amino acid sequence that is identical to the sequence of SEQ ID NO: 6.
10. The method of claim 1 , wherein the polynucleotide is administered in a pharmaceutical preparation.
11. The method of claim 1 , wherein the patient has anemia associated with chronic kidney disease.
12. The method of claim 1 , wherein the patient has anemia associated with a chemotherapy treatment.
13. The method of claim 1 , wherein the chemotherapy treatment is a taxane.
14. The method of claim 1 , wherein the patient has anemia as a consequence of blood loss.
15. The method of claim 1 , wherein the BMP9 or BMP10 polypeptide encoded by the polynucleotide stimulates SMAD 1/5/8 signaling.
16. The method of claim 1 , wherein the polynucleotide is in a viral vector.
17. The method of claim 1 , wherein the polynucleotide is in a liposome.