In vivo generation of water-soluble cannabinoids in plant cell suspension cultures
The present invention includes systems, methods and compositions for the in vivo bioconversion of water-soluble cannabinoids in plant cell suspension cultures.
1. A method of glycosylating a cannabinoid, comprising the steps of:
(a) establishing a tobacco cell suspension culture of Nicotiana tabacum or Nicotiana benthamiana cells;
(b) introducing into said tobacco cell suspension culture a cannabinoid, wherein the cannabinoid is cannabidiol (CBD) or cannabidiolic acid (CBDA);
(c) incubating said cannabinoid in said tobacco cell suspension culture, thereby glycosylating the cannabinoid;
(d) isolating a cannabinoid glycoside from said tobacco cell suspension culture, wherein the cannabinoid glycoside is a CBD-glycoside or a CBDA-glycoside.