METHOD FOR GROWING FUNGAL MYCELIUM AND FORMING EDIBLE PRODUCTS THEREFROM
A method of growing fungal mycelium and forming edible food products includes growing fungal cells in a growth media such that the fungal cells produce mycelium. The growth media includes a sugar, a nitrogen-containing compound, and a phosphate-containing compound. The mycelium is separated from the growth media. The mycelium is concentrated to obtain a fibrous mycelium mass having a protein content of greater than 40 wt % of a dry mass of the mycelium. Food additives can be added to the fibrous mycelium mass to produce a biomass. The biomass can be formed into an edible food product. The biomass can be formed into an edible meat substitute product.
1 . A method, comprising:
growing fungal cells in a growth media such that the fungal cells produce mycelium, the growth media comprising a sugar, a nitrogen-containing compound, and a phosphate-containing compound;
separating the mycelium from the growth media; and
concentrating the mycelium to obtain a fibrous mycelium mass having a protein content of greater than 40 wt % of a dry mass of the mycelium.
2 . The method of claim 1 , further comprising:
removing 0.01 vol % to 95 vol % of a broth comprising the fungal cells and the growth media; and
adding fresh growth media to a container containing the broth.
3 . The method of claim 1 , wherein the sugar is in a range of 5-50 g/L, the nitrogen-containing compound is in a range of 0.5-10 g/L, and the phosphate-containing compound is in a range of 0.1-5 g/L.
4 . The method of claim 1 , wherein the sugar comprises at least one of sucrose, glucose, fructose, or molasses.
5 . The method of claim 1 , wherein the nitrogen-containing compound comprises at least one of ammonium hydroxide, ammonium nitrate, ammonium sulfate, ammonium chloride, urea, yeast extract, or peptone.
6 . The method of claim 1 , wherein the phosphorous-containing compound comprises at least one of potassium phosphate, sodium phosphate, or phosphoric acid.
7 . The method of claim 1 , further comprising:
lysing cell walls of the mycelium.
8 . The method of claim 1 , further comprising:
adding food additives to the mycelium, the food additives comprising at least one of vegetable proteins, animal proteins, fats, emulsifiers, thickeners, stabilizers, or flavorings.
9 . A method of forming an edible food product comprising:
providing a fibrous mycelium mass having a protein content of greater than 40 wt % of a dry mass of the mycelium;
adding food additives to the fibrous mycelium mass to produce a biomass; and
forming the biomass into an edible food product.
10 . The method of claim 9 , wherein forming the biomass into an edible food product further comprises:
dehydrating the biomass; and
pulverizing the biomass into a powder.
11 . The method of claim 10 , further comprising:
incorporating the powder into a baked food product.
12 . The method of claim 9 , wherein the edible food product is at least one of a chip, a protein bar, a jerky, a tortilla, a bread, or a cracker.
13 . The method of claim 9 , further comprising:
molding the biomass into a shape.
14 . A method of forming an edible meat substitute product comprising:
providing a fibrous mycelium mass having a protein content of greater than 40 wt % of a dry mass of the mycelium;
adding food additives to the fibrous mycelium mass to produce a biomass; and
forming the biomass into the edible meat substitute product.
15 . The method of claim 14 , wherein forming the biomass into the edible meat substitute product comprises:
adding at least one soluble protein to the biomass; and
molding the biomass into the edible meat substitute product.
16 . The method of claim 14 , wherein forming the biomass into the edible meat substitute product comprises:
adding at least one thickener to the biomass; and
molding the biomass into the edible meat substitute product.
17 . The method of claim 14 , wherein forming the biomass into the edible meat substitute product comprises:
adding at least one fat source to the biomass; and
molding the biomass into the edible meat substitute product.
18 . The method of claim 14 , further comprising:
adding seasonings to the biomass.
19 . The method of claim 14 , wherein the edible meat substitute product comprises at least one of a chicken substitute product, a beef substitute product, a pork substitute product, a veal substitute product, or a fish substitute product.
20 . The method of claim 14 , wherein the edible meat substitute product comprises 10 wt % to 90 wt % of the fibrous mycelium mass.