Granted Patent
B2
US 10,683,490 · App. 16/440,241 · Granted Jun 16, 2020
Mutant Cas9 proteins
Inventors:
Alejandro Chavez (New York, NY); Frank J. Poelwijk (Dallas, TX); George M. Church (Brookline, MA)
Assignee:
President and Fellows of Harvard College
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Abstract
Methods of making mutant Cas9 proteins are described.
Claims (5)
1. A mutant Neisseria meningitides Cas9 protein (NM-Cas9) lacking one or more stretches of amino acid sequences within a family of Cas9 proteins, wherein the one or more stretches of amino acid sequences are of low conservation or are between conservation edges, and wherein the mutant NM-Cas9 protein has DNA binding activity.
2. The mutant NM-Cas9 protein of claim 1 having nuclease activity.
3. The mutant NM-Cas9 protein of claim 1 having nickase activity.
4. The mutant NM-Cas9 protein of claim 1 being nuclease null.
5. The mutant NM-Cas 9 protein of claim 1 including one or more SGGGS linkers.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jun 17, 2019
From: CHAVEZ, ALEJANDRO; CHURCH, GEORGE M.; POELWIJK, FRANK J.
To: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Reel/Frame 049482/0530 →
Continuity (4)
Division
15037696
Provisional Application
61906374
· Nov 19, 2013
Cited By (3)