Typing method
The invention provides a method of determining the epigenetic chromosome interactions which are relevant to an epigenetic test for a neurodegenerative condition.
1. A method of identifying a human individual in need of therapy for Alzheimer's disease and treating the identified human individual, the method comprising:
(A) assaying a blood sample from a human individual for ten chromosome interactions, wherein said assaying comprises the steps of:
(a) performing in vitro cross-linking of chromosome regions of the human individual which have come together in a chromosome interaction to form cross- linked nucleic acid;
(b) subjecting said cross-linked nucleic acid to restriction digestion cleavage with an enzyme to form cross-linked cleaved nucleic acids;
(c) ligating said cross-linked cleaved nucleic acid ends to form a ligated nucleic acid; and
(d) detecting the ligated nucleic acid to determine the presence or absence of a chromosome interaction and wherein the ten chromosome interactions are the following chromosomal interactions:
(i) the chromosome interaction on chromosome 12 that results in a ligated nucleic acid comprising SEQ ID NO: 8;
(ii) the chromosome interaction on chromosome 8 that results in a ligated nucleic acid comprising SEQ ID NO: 1;
(iii) the chromosome interaction on chromosome 8 that results in a ligated nucleic acid comprising SEQ ID NO: 19;
(iv) the chromosome interaction on chromosome 3 that results in a ligated nucleic acid comprising SEQ ID NO: 5;
(v) the chromosome interaction on chromosome 8 that results in a ligated nucleic acid comprising SEQ ID NO: 2;
(vi) the chromosome interaction on chromosome 10 that results in a ligated nucleic acid comprising SEQ ID NO: 24;
(vii) the chromosome interaction on chromosome 9 that results in a ligated nucleic acid comprising SEQ ID NO: 6;
(viii) the chromosome interaction on chromosome 11 that results in a ligated nucleic acid comprising SEQ ID NO:20;
(ix) the chromosome interaction on chromosome 3 that results in a ligated nucleic acid comprising SEQ ID NO:21; and
(x) the chromosome interaction on chromosome 7 that results in a ligated nucleic acid comprising SEQ ID NO:15;
(B) identifying the human individual as being in need of therapy for Alzheimer's disease when one or more of chromosome interactions (i)-(vii) are present and/or one or more of chromosome interactions (viii)-(x) are absent; and
(C) administering to the identified human individual an agent that is therapeutic for Alzheimer's disease.