Films for biologic analyte collection and analysis and methods of production and use thereof
Compositions, films, collection devices, apparatuses, kits and methods related to biologic analyte collection and analysis include thin films of modified polycaprolactone. Methods of production and use thereof are described herein. The films, compositions, collection devices, kits and methods can be used for collection of fingerprints for both image capture and nucleic acid extraction and analysis.
1. A method of collecting and analyzing a biologic analyte comprising:
contacting a transparent or semi-transparent hydrophilic thin film with a sample comprising a biologic analyte, the transparent or semi-transparent hydrophilic thin film consisting essentially of polycaprolactone and a backing applied or adhered to one side of the film, the sample reversibly adhered to the polycaprolactone, the polycaprolactone having been treated with a base having a pH greater than 8 and a neutralizing agent having a thickness in the range of about 0.01 mm to about 0.6 mm that provides greater than 75% blood DNA uptake and release, wherein at least a portion of the hydrophilic film solubilizes when exposed to at least one reagent for extraction of biological analytes, and wherein the hydrophilic film has been sterilized such that it is free of nucleic acids;
contacting the transparent or semi-transparent hydrophilic thin film and the sample with at least one biologic analyte extraction reagent under conditions such that the polycaprolactone is solubilized and the sample is separated from the transparent or semi-transparent hydrophilic thin film;
collecting the separated sample; and
separating the biologic analyte from the sample.
2. The method of claim 1 , wherein the biologic analyte comprises at least one nucleic acid, and wherein separating the biologic analyte from the sample comprises:
subjecting the separated sample comprising the biologic analyte to a nucleic acid extraction reagent, and
extracting the at least one nucleic acid from the separated sample.
3. The method of claim 2 , wherein the at least one nucleic acid comprises eukaryotic genomic DNA, prokaryotic genomic DNA, oligonucleotide, mitochondrial DNA, cDNA, short tandem repeat (STR), bacterial plasmid, or bacteriophage DNA.
4. The method of claim 1 , wherein the sample comprises cells, tissue, blood, urine, semen, lymphatic fluid, cerebrospinal fluid, amniotic fluid, fingerprint, buccal swab, mouthwash, stool, tissue culture cells, tissues slices, tumor biopsy, or biopsy aspirate.
5. The method of claim 1 , further comprising analyzing the biologic analyte, wherein analyzing the biologic analyte comprises at least one selected from the group consisting of: nucleic acid sequencing, forensic analysis, comparing the sample to those contained in the Combined DNA Index System (CODIS), protein assay, chemical analysis, immunoassay, mass spectrometry, microarray analysis, and detection of radioactive material.
6. The method of claim 1 , further comprising analyzing the sample, wherein the sample is a fingerprint.
7. A method of genotyping a sample comprising a nucleic acid, the method comprising:
collecting or providing a sample comprising a nucleic acid using the transparent or semi-transparent hydrophilic thin film, the sample reversibly adhered to the transparent or semi-transparent hydrophilic film, the transparent or semi-transparent hydrophilic thin film consisting essentially of polycaprolactone and a backing applied or adhered to one side of the film, the polycaprolactone having been treated with a base having a pH greater than 8 and a neutralizing agent having a thickness in the range of about 0.01 mm to about 0.6 mm that provides greater than 75% blood DNA uptake and release, wherein at least a portion of the hydrophilic film solubilizes when exposed to at least one reagent for extraction of biological analytes, and wherein the hydrophilic film has been sterilized such that it is free of nucleic acids, wherein the sample is reversibly adhered to the hydrophilic film;
contacting the transparent or semi-transparent hydrophilic thin film and the sample with at least one nucleic acid extraction reagent under conditions such that the polycaprolactone is solubilized and the sample is separated from the transparent or semi-transparent hydrophilic thin film;
separating the nucleic acid from the sample; and
analyzing the nucleic acid for a plurality of genetic markers at a plurality of STR loci and generating a DNA profile that may be compared to at least one other DNA profile in at least one DNA database.
8. The method of claim 7 , wherein the sample is a human buccal sample or blood sample, and wherein comparing the DNA profile to the at least one other DNA profile in at least one DNA database comprises use of CODIS software.
9. The method of claim 7 , wherein the sample is a fingerprint directly applied to the transparent or semi-transparent hydrophilic thin film or a fingerprint from at least one surface of forensic interest selected from the group consisting of: a crime scene and a contraband material.
10. The method of claim 7 , wherein the sample is obtained from a fingerprint directly applied to the transparent or semi-transparent hydrophilic thin film or from a fingerprint from at least one surface of forensic interest selected from the group consisting of: a crime scene and a contraband material.