Mushroom line J14756-s3 and methods and uses therefor
A mushroom culture of Agaricus bisporus , comprising at least one set of chromosomes as a culture of line J14756-s3, includes a representative culture of the line, which has been deposited under NRRL Accession No. 67317. A method of producing a hybrid mushroom culture of Agaricus bisporus comprising: mating a culture comprising at least one set of chromosomes as a culture of line J14756-s3 with a second homokaryotic line culture. Additionally, mushrooms, parts of the culture and products incorporating the culture of the invention are provided.
1. An Agaricus bisporus culture comprising:
at least one set of chromosomes of an Agaricus bisporus line J14756-s3, a culture of the line J14756-s3 having been deposited under the NRRL Accession Number 67317, wherein said chromosomes comprise all alleles reported for the line J14756-s3 at the sequence-characterized marker loci listed in at least one of Tables I and II, wherein Table I is provided as follows:
TABLE I
Scaffold
Position of SNP
Cultures:
[H97 V2.0 ref. coordinates]
H97
Wild Line
1
99995
CTACATTGA
CTACGTTGA
1
349966
AAGGTGGTT
AAGGCGGTT
1
600059
TTTTTTTT-
TTTTTTTTC
1
850014
CCTTTTCAC TCTTGTCAC
TCTTGTCACTCTTGTCAC
1
1099971
GTCGACACC
GTCGACACC
1
1350278
GGAGAGTCG
GGAGAGTCG
1
1599956
AATAAGCGC
AATAAGCGC
1
1850032
CGAGTAATT
CGAGCAATT
1
2119049
ACAATCCAA
ACAACTCAA
1
2400243
ACTTCATGA
ACTTGATGA
1
2612870
AATAGGAGT
AATAGGAGT
1
2858975
GCCGTTCTTGCCGCTCTTG
CCGCTCTTGCCGCTCTT
1
2804522
GAAGACGAC
GAAGGCGAC
1
3047987
AAGGGGGGG
AAGGAGGGG
1
3212182
CACTACGTG
CACTGCGTG
1
3256057
TATCTGTTT TATCCGTTT
TATCCGTTT TATCCGTTT
2
101820
ATTAAAGAT
ATTAAAGAT
2
350156
TCGGGGGTG
TCGGAGGTG
2
600112
ATGTATACG
ATGTGTACG
2
850338
TGGTGCTAA
TGGTTCTAA
TGGTGCTAA
2
1099413
CCTGACTCA
CCTGGCTCA
2
1349512
CTCAGCAGT
CTCAACAGT
2
1600085
CACAATGCC
CACATTGCC
2
1901773
ACTCGAATT
ACTAAAATT
2
2150201
GTCGTAGGT
GTCGAAGGT
2
2400281
TCAAAACCC
TCAACACCC
2
2650136
ATAATTCCTATAAGTCCT
2
2903620
ACTAAAAGA
ACTAAAAGA
2
3048019
GTCCGCTGC
GTCCGCTGC
3
57117
TATAGCAGT
TATGACAGT
3
65650
GGCGCTTTTGGCGGTTTTG
GCGGTTTTGGCGGTTTT
3
119181
TTTATACTCTTTATACTCT
TTATACTCTTTATACTC
3
250112
GCAGGAGAG
GCCGAAGAG
3
750000
GTCCGGCCA
GTCCGGCCA
3
750074
AGTTTTTTC AGTTATTTC
AGTTTTTTC AGTTwTTTC
3
1250000
TTTTTCCGG TTTTTCCGG
TTTTTCCGG TTTTTCCGG
3
1250161
AGTCTCCTT AGTCCCCTT
AGTCTCCTT AGTCyCCTT
3
1750000
ACGCCTGAC
ACGCCTGAC
3
1750033
TTTTTTGCT TTTTGTGCT
TTTTTTGCT TTTTkTGCT
3
2250000
CGTGGCGAT
CGTGGCGAT
3
2250027
TGTATCAAG
TGTGCCAAG
3
2520748
TAATTCCAC
TAATGCCAC
4
100004
GAGTGATAA
GAGTGATAA
4
490648
CGATCGCGT
CGATTGCGT
4
598147
GATCGACAG
GATCAACAG
4
852119
CGAATATTC
CGAATATTC
4
1100085
GATGCCGAA
GATGACGAA
4
1350536
CGAACTCGG
CGAAATCGG
4
1599885
GATACTTGC
GATACTTGC
4
1850288
ATTCGTGTAATTCATGTAA
TTCGTGTAATTCrTGTA
4
2100356
TCAGAGACC
TCAGAGACC
4
2284257
TCTGGACTG
TCTGAACTG
5
100211
TCCTTGAATTCCTGGAAT
5
350872
GGCGTGCCC
GGCGTGCCC
5
599922
CGTCATTCA
CGTCATTCA
5
851262
TAATTCTCTTAATCGTCTT
AATCGTCTTAATCGTCT
5
1099776
ACATTGACA
ACATCGACA
5
1352539
TTGTGATCCTTGTGATCCT
TGTTGTCCTTGTkrTCC
5
1599904
AACTTCCTT AACTCCCTT
AACTCCCTT AACTCCCTT
5
1851458
AAATAATCC
AAATTCTCC
5
2100025
CCCTTAGTCCCCTTAGTCC
CCTCAGTC
5
2278878
GGTCAAAAA
GGTCAAAAA
6
106294
GCCATCTCG
GCCACCTCG
6
350337
CATTTGGTTCATTCGGTTC
ATTTGGTTCATTyGGTT
6
600047
GGAGCATTT
GGAGTATTT
6
849985
AGTTCAGGA
AGTTCAGGA
6
1098535
CAAAGATTG
CAAAAATTG
6
1349453
TGTCGGTAG
TGTCAATAG
6
1600000
AAACCTGGA
AAACCTGGA
6
1764645
AACCAGATT
AACCGGATT
6
2000087
GATTTTGCGGATTTTGCGG
ATTTTGCGGATTTTGCG
6
2252662
GGGTTGGTA
GGGTCGGTA
7
100284
GAAATTCAG
GAAATTCAG
7
350044
ATATTCTTT ATATCCTTT
ATATTCTTT ATATyCTTT
7
600111
CAATTATTACAATCATTA
7
850246
TGACGCATA
TGACGCATA
7
1100248
TCACGGAAG
TCACGGAAG
7
1350089
CTTTTCCCC CTTTTCCCCC
TTTTCCCC CTTTTCCCC
7
1605047
ATACTTGGC
ATACTTGAC
7
1860993
GTCAACCGG
GTCATCCGG
7
1898793
TCCGCATAA
TCCGCATAA
7
1991505
TCTACGGTTTCTACGGTTT
CTACGGTTTCTACGGTT
8
350000
ATTGACGCG
ATTGGCGCG
8
610549
GAACTTGAT
GAATACGAT
8
1100000
CATACGATC
CATACGATC
8
1100546
GCCCCAGAA
GCCCCAGAA
8
1350000
AGCTTAACA
AGCTTAACA
8
1350240
ACGGGTACT
ACGGATACT
8
1600100
CTGAACCCT
CTGAACCCT
9
100105
CTCAACCGA
CTCAACCGA
9
352455
AGTCCTCCA
AGTCCTCCA
9
599950
TGGTATCCC
TGGTGTCCC
9
1010845
GGGTGGTGA
GGGTGGTGA
9
1244202
GATGAAGAT
GATGAAGAT
9
1504476
TACTGTACC
TACTGTACC
9
1656962
TATCTACTGTATCTACTGT
ATCTACTGTATCTACTG
10
100438
AATTAATTTGATTAATTTA
ATTAATTTrATTAATTT
10
350030
GCGGTTCAA
GCGGTTCAA
10
600032
TTACACTGG
TTACACTGG
10
850000
TCGGTCGGA
TCGGTCGGA
10
860249
CCGCAAATT
CCGCGAATT
10
1173178
ATCCCCAAT
ATCCTCAAT
10
1303902
TGATTTACTTGATTTACTT
GATTTACTTGATTTACT
10
1490452
AATCAGATG
AATCAGATG
11
101855
CCAGCCTGT
CCAGTCTGT
11
350000
GTCAGCAAG
GTCAGCAAG
11
350025
GATAAAACA
GATAAAACA
11
600000
ATGGGCGCG
ATGGGAGCG
11
929659
GGAATATCA
GGAAGTTCA
11
1100608
AGTGGTCTTAGTGGTCTTA
GTGATCTTAGTGrTCTT
11
1240230
ACAAGTTTC
ACAAGTTTC
12
100000
CCTTCTAGTCCTTCTAGTC
CTTCTAGTCCTTCTAGT
12
109790
GTCTGCACC
GTCTACACC
12
1000000
CGAGGAGGA
CGAGGAGGA
13
100697
ACGTCTTTAACGTCTTTAA
CGTCTTTAACGTCTTTA
13
370521
TTTGAGTCATTTGTGTCAT
TTGTGTCATTTGTGTCA
13
604345
CTTCAGCAT
CTTCAGCAT
13
850249
GGCTAGTAA
GGCTAGTAA
14
113109
AGGGAAATA
AGGGGAATA
14
372086
CGATCCCTTCGATTCCTTC
GATCCCTTCGATyCCTT
14
725684
ATGAGTTCG
ATGAGTTTG
15
150013
GTGGCCCGT
GTGGACCGT
15
449860
GAATTTCGG
GAATTTCGG
16
205778
CAAGGTCTG
CAAGATCTG
16
400000
CCTCGGATT
CCTCGGATT
16
403998
CCAAGTACG
CCAAATACG
17
120000
TATTCTTCA TATTCTTCA
TATTCTTCA TATTCTTCA
17
134676
TTGCCCAGC
TTGCTCAGC
17
338415
TGAGAAGCC
TGAGAAGCC
17
449833
ATCAAACAA
ATCAAACAA
18
101884
ATTACGGAC
ATTACGGAC
18
112940
GCGGGTGGG
GCGGCTGGG
19
98520
GCTATTGGG
GCTATTGGG
19
98782
AAAATTGTT
AAAAGTGTT
Scaffold
Position of SNP
Cultures:
[H97 V2.0 ref. coordinates]
J14756-s3
J15051
1
99995
CTACGTTGA
CTACGTTGA
1
349966
AAGGCGGTT
AAGGCGGTT
1
600059
TTTTCTTT-
TTTTyTTTC
1
850014
1
1099971
GTCGGCACC
GTCGrCACC
1
1350278
GGAGGTTCG
GGAGrkTCG
1
1599956
AATAGGCGC
AATArGCGC
1
1850032
CGAGCAATT
CGAGCAATT
1
2119049
ACAACTCAA
ACAACTCAA
1
2400243
ACTTGATGA
ACTTGATGA
1
2612870
AATAAGAGT
AATArGAGT
1
2858975
1
2804522
GAAGGCGAC
GAAGGCGAC
1
3047987
AAGGGGGGG
AAGGrGGGG
1
3212182
CACTACGTG
CACTrCGTG
1
3256057
2
101820
ATTAAAGAT
ATTAAAGAT
2
350156
TCGGGGGTG
TCGGrGGTG
2
600112
ATGTATACG
ATGTrTACG
2
850338
TGGTkCTAA
2
1099413
CCTGACTCA
CCTGrCTCA
2
1349512
CTCAGCAGT
CTCArCAGT
2
1600085
CACAATGCC
CACAwTGCC
2
1901773
ACTCAAATT
ACTmAAATT
2
2150201
GTCGTAGGT
GTCGwAGGT
2
2400281
TCAACACCC
TCAACACCC
2
2650136
ATAATTCCT
ATAAkTCCT
2
2903620
ACTAAAAGA
ACTAAAAGA
2
3048019
GTCCGCTGC
GTCCGCTGC
3
57117
TATGACAGT
TATGACAGT
3
65650
3
119181
3
250112
GCAGGAGAG
GCmGrAGAG
3
750000
GTCCGGCCA
GTCCGGCCA
3
750074
3
1250000
3
1250161
3
1750000
ACGCCTGAC
ACGCCTGAC
3
1750033
3
2250000
CGTGGCGAT
CGTGGCGAT
3
2250027
TGTGCCAAG
TGTryCAAG
3
2520748
TAATTCCAC
TAATkCCAC
4
100004
GAGTGATAA
GAGTGATAA
4
490648
CGATCGCGT
CGATyGCGT
4
598147
GATCGACAG
GATCrACAG
4
852119
CGAATATTC
CGAATATTC
4
1100085
GATGCCGAA
GATGmCGAA
4
1350536
CGAACTCGG
CGAAmTCGG
4
1599885
GATACTTGC
GATACTTGC
4
1850288
4
2100356
TCAGGGACC
TCAGrGACC
4
2284257
TCTGAACTG
TCTGrACTG
5
100211
TCCTGGAAT
TCCTGGAAT
5
350872
GGCGCGCCC
GGCGyGCCC
5
599922
CGTCGTTCA
CGTCrTTCA
5
851262
5
1099776
ACATTGACA
ACATyGACA
5
1352539
5
1599904
5
1851458
AAATTCTCC
AAATTCTCC
5
2100025
CCCTyAGTC
5
2278878
AGTCAAAAA
rGTCAAAAA
6
106294
GCCATCTCG
GCCAyCTCG
6
350337
6
600047
GGAGCATTT
GGAGyATTT
6
849985
AGTTCAGGA
AGTTCAGGA
6
1098535
CAAAGATTG
CAAArATTG
6
1349453
TGTCGGTAG
TGTCrrTAG
6
1600000
AAACCTGGA
AAACCTGGA
6
1764645
AACCGGATT
AACCrGATT
6
2000087
6
2252662
GGGTTGGTA
GGGTyGGTA
7
100284
GAAACTCAG
GAAAyTCAG
7
350044
7
600111
CAATTATTA
CAATyATTA
7
850246
TGACGCATA
TGACGCATA
7
1100248
TCACGGAAG
TCACrGAAG
7
1350089
7
1605047
ATACTTGGC
ATACTTGrC
7
1860993
GTCAACCGG
GTCAwCCGG
7
1898793
TCCGCATAA
TCCGCATAA
7
1991505
8
350000
ATTGGCGCG
ATTGGCGCG
8
610549
GAATTTGAT
GATwyGAT
8
1100000
CATACGATC
CATACGATC
8
1100546
GCCCGAGAA
GCCCsAGAA
8
1350000
AGCTTAACA
AGCTTAACA
8
1350240
ACGGGTACT
ACGGrTACT
8
1600100
CTGAACCCT
CTGAACCCT
9
100105
CTCAGCCGA
CTCArCCGA
9
352455
AGTCTCCCA
AGTCyyCCA
9
599950
TGGTGTCCC
TGGTGTCCC
9
1010845
GGGTAGTGA
GGGTrGTGA
9
1244202
GATGGAGAT
GATGrAGAT
9
1504476
TACTATACC
TACTrTACC
9
1656962
10
100438
10
350030
GCGGTTCAA
GCGGTTCAA
10
600032
TTACACTGG
TTACACTGG
10
850000
TCGGTCGGA
TCGGTCGGA
10
860249
CCGCAAATT
CCGCrAATT
10
1173178
ATCCTCAAT
ATCCTCAAT
10
1303902
10
1490452
AATCAGATG
AATCAGATG
11
101855
CCAGCCTGT
CCAGyCTGT
11
350000
GTCAGCAAG
GTCAGCAAG
11
350025
GATATAACA
GATAwAACA
11
600000
ATGGGAGCG
ATGGGrGCG
11
929659
GGAATATCA
GGAAkwTCA
11
1100608
11
1240230
ACAAATTTC
ACAArTTTC
12
100000
12
109790
GTCTGCACC
GTCTrCACC
12
1000000
CGAGAAGGA
CGAGrAGGA
13
100697
13
370521
13
604345
CTTCAGCAT
CTTCAGCAT
13
850249
GGCTAGTAA
GGCTAGTAA
14
113109
AGGGAAATA
AGGGrAATA
14
372086
14
725684
ATGAATTCG
ATGArTTyG
15
150013
GTGGCCCGT
GTGGmCCGT
15
449860
GAATTTCGG
GAATTTCGG
16
205778
CAAGATCTG
CAAGATCTG
16
400000
CCTCGGATT
CCTCGGATT
16
403998
CCAAGTACG
CCAArTACG
17
120000
17
134676
TTGCCCAGC
TTGCyCAGC
17
338415
TGAGAAGCC
TGAGAAGCC
17
449833
ATCAAACAA
ATCAAACAA
18
101884
ATTACGGAC
ATTACGGAC
18
112940
GCGGGTGGG
GCGGsTGGG
19
98520
GCTATTGGG
GCTATTGGG
19
98782
AAAATTGTT
AAAAkTGTT
and wherein Table II is provided as follows:
TABLE II
Markers:
p1n150/
MFPC-
Match
Strain:
Mat
1-ELF
FF
AN
AS
ITS
%
J15051
2/5
E3/E4
FF2/
N3/
SA/
I1/
[100%]
FF2
N4
SD
I5
B14528
2/5
E3/E4
FF1/
N3/
SC/
I1/
66.7%
FF3
N4
SD
I2
28c, [Heirloom]
1T/5
E3/E4
FF1/
N2/
SA/
I1/
58.3%
FF3
N3
SD
I1
28b, [Braun]
1T/5
E3/E4
FF1/
N2/
SB/
I1/
50.0%
FF4
N3
SD
I1
PTA-6877
1T/2
E2/E3
FF1/
N2/
SD/
I1/
33.3%
FF1
N4
SD
I2
S600
1T/2
E1/E3
FF2/
N2/
SC/
I1/
41.7%
FF2
N4
SD
I2
J10263
1T/2
E1/E3
FF2/
N4/
SC/
I1/
50.0%
FF7
N7
SD
I2
OFB lineage group
1T/3
E3/E6
FF1/
N4/
SC/
I1/
41.7%
FF2
N4
SD
I3
J14756-s3
2
E3
FF2
N4
SD
I5
wild line
5
E4
FF2
N3
SA
I1.
2. The Agaricus bisporus culture of claim 1 , wherein said culture is an F1 hybrid mushroom culture produced by mating a culture of the line J14756-s3 with a different Agaricus bisporus culture.
3. The Agaricus bisporus culture of claim 2 , wherein said F1 hybrid mushroom culture is vegetatively incompatible with all strains selected from the group consisting of Old Fashioned Brown strains, SB-65, SB-295, 2400, S-600/X618, BR06/“Heirloom”, B14528/“Tuscan”, Broncoh, and J10263/“Forestiere”.
4. An Essentially Derived Variety culture derived from an initial culture which is the culture of claim 1 , wherein the Essentially Derived Variety culture has at least 75% genotypic and genomic identity with the culture of the line J14756-s3.
5. A part of the culture of claim 1 selected from the group consisting of hyphae and cells.
6. A product comprising the Agaricus bisporus culture of claim 1 , wherein the product is selected from the group consisting of mycelium, spawn, inoculum, casing inoculum, fresh mushrooms, processed mushrooms, mushroom pieces, and colonized substrates including grain, compost, and friable particulate matter.
7. A method for developing a resultant culture in a mushroom strain development program comprising:
applying at least one mushroom strain development technique to a first mushroom culture, or parts thereof, wherein said first mushroom culture is a culture comprising at least one set of chromosomes of Agaricus bisporus line J14756-s3, wherein a culture of said line J14756-s3 having been deposited under the NRRL Accession Number 67317, to provide the second culture.
8. The method for developing the resultant culture in the mushroom strain development program of claim 7 , wherein mushroom strain development techniques are selected from the group consisting of inbreeding, outbreeding, selfing, backcrossing, introgressive trait conversion, essential derivation, somatic selection, single-spore selection, multispore selection, pedigree-assisted breeding, marker assisted selection, mutagenesis and transformation.
9. A method of producing a hybrid mushroom culture comprising:
(a) growing a progeny culture produced by mating the culture of claim 1 with a second Agaricus bisporus culture;
(b) mating the progeny culture with a different culture or with itself via at least one offspring line to produce a progeny culture of a subsequent generation;
(c) growing a progeny culture of a subsequent generation and mating the progeny culture of a subsequent generation with itself or a different culture; and
(d) repeating steps (b) and (c) for an additional 0-5 generations to produce a mushroom culture.
10. A cell of the Agaricus bisporus culture of claim 1 , further comprising a marker profile in accordance with the profile of line J14756-s3 shown in Table I.
11. A process for introducing a desired trait into a culture of Agaricus bisporus line J14756-s3 comprising the steps of:
(a) physically mating the culture of Agaricus bisporus line J14756-s3, wherein a culture of said line J14756-s3 has been deposited under the NRRL Accession Number 67317, to a second culture of Agaricus bisporus having the desired trait, to produce a hybrid;
(b) obtaining an offspring of the hybrid that carries at least one gene that determines the desired trait from the hybrid;
(c) mating said offspring of the hybrid with the culture of Agaricus bisporus line J14756-s3 to produce a new hybrid;
(d) repeating steps (b) and (c) at least once to produce a subsequent hybrid;
(e) obtaining a homokaryotic line carrying at least one gene that determines the desired trait and comprising at least 75% of the alleles of line J14756-s3, at sequence-characterized marker loci described in at least one of Tables I and II, wherein Table I is provided as follows:
TABLE I
Scaffold
Position of SNP
Cultures:
[H97 V2.0 ref. coordinates]
H97
Wild Line
J14756-s3
J15051
1
99995
CTACATTGA
CTACGTTGA
CTACGTTGA
CTACGTTGA
1
349966
AAGGTGGTT
AAGGCGGTT
AAGGCGGTT
AAGGCGGTT
1
600059
TTTTTTTT- TTTTTTTTC TTTTCTTT- TTTTyTTTC
1
850014
CCTTTTCAC TCTTGTCACTCTTGTCACTCTTGTCAC
1
1099971
GTCGACACC
GTCGACACC
GTCGGCACC
GTCGrCACC
1
1350278
GGAGAGTCG
GGAGAGTCG
GGAGGTTCG
GGAGrkTCG
1
1599956
AATAAGCGC
AATAAGCGC
AATAGGCGC
AATArGCGC
1
1850032
CGAGTAATT
CGAGCAATT
CGAGCAATT
CGAGCAATT
1
2119049
ACAATCCAA
ACAACTCAA
ACAACTCAA
ACAACTCAA
1
2400243
ACTTCATGA
ACTTGATGA
ACTTGATGA
ACTTGATGA
1
2612870
AATAGGAGT
AATAGGAGT
AATAAGAGT
AATArGAGT
1
2858975
GCCGTTCTT GCCGCTCTTGCCGCTCTTGCCGCTCTT
1
2804522
GAAGACGAC
GAAGGCGAC
GAAGGCGAC
GAAGGCGAC
1
3047987
AAGGGGGGG
AAGGAGGGG
AAGGGGGGG
AAGGrGGGG
1
3212182
CACTACGTG
CACTGCGTG
CACTACGTG
CACTrCGTG
1
3256057
TATCTGTTT TATCCGTTT TATCCGTTT TATCCGTTT
2
101820
ATTAAAGAT
ATTAAAGAT
ATTAAAGAT
ATTAAAGAT
2
350156
TCGGGGGTG
TCGGAGGTG
TCGGGGGTG
TCGGrGGTG
2
600112
ATGTATACG
ATGTGTACG
ATGTATACG
ATGTrTACG
2
850338
TGGTGCTAA
TGGTTCTAATGGTGCTAA
TGGTkCTAA
2
1099413
CCTGACTCA
CCTGGCTCA
CCTGACTCA
CCTGrCTCA
2
1349512
CTCAGCAGT
CTCAACAGT
CTCAGCAGT
CTCArCAGT
2
1600085
CACAATGCC
CACATTGCC
CACAATGCC
CACAwTGCC
2
1901773
ACTCGAATT
ACTAAAATT
ACTCAAATT
ACTmAAATT
2
2150201
GTCGTAGGT
GTCGAAGGT
GTCGTAGGT
GTCGwAGGT
2
2400281
TCAAAACCC
TCAACACCC
TCAACACCC
TCAACACCC
2
2650136
ATAATTCCTATAAGTCCT
ATAATTCCT
ATAAkTCCT
2
2903620
ACTAAAAGA
ACTAAAAGA
ACTAAAAGA
ACTAAAAGA
2
3048019
GTCCGCTGC
GTCCGCTGC
GTCCGCTGC
GTCCGCTGC
3
57117
TATAGCAGT
TATGACAGT
TATGACAGT
TATGACAGT
3
65650
GGCGCTTTTGGCGGTTTTGGCGGTTTTGGCGGTTTT
3
119181
TTTATACTCTTTATACTCTTTATACTCTTTATACTC
3
250112
GCAGGAGAG
GCCGAAGAG
GCAGGAGAG
GCmGrAGAG
3
750000
GTCCGGCCA
GTCCGGCCA
GTCCGGCCA
GTCCGGCCA
3
750074
AGTTTTTTC AGTTATTTC AGTTTTTTC AGTTwTTTC
3
1250000
TTTTTCCGG TTTTTCCGG TTTTTCCGG TTTTTCCGG
3
1250161
AGTCTCCTT AGTCCCCTT AGTCTCCTT AGTCyCCTT
3
1750000
ACGCCTGAC
ACGCCTGAC
ACGCCTGAC
ACGCCTGAC
3
1750033
TTTTTTGCT TTTTGTGCT TTTTTTGCT TTTTkTGCT
3
2250000
CGTGGCGAT
CGTGGCGAT
CGTGGCGAT
CGTGGCGAT
3
2250027
TGTATCAAG
TGTATCAAG
TGTGCCAAG
TGTryCAAG
3
2520748
TAATTCCAC
TAATGCCAC
TAATTCCAC
TAATkCCAC
4
100004
GAGTGATAA
GAGTGATAA
GAGTGATAA
GAGTGATAA
4
490648
CGATCGCGT
CGATCGCGT
CGATTGCGT
CGATyGCGT
4
598147
GATCGACAG
GATCAACAG
GATCGACAG
GATCrACAG
4
852119
CGAATATTC
CGAATATTC
CGAATATTC
CGAATATTC
4
1100085
GATGCCGAA
GATGACGAA
GATGCCGAA
GATGmCGAA
4
1350536
CGAACTCGG
CGAAATCGG
CGAACTCGG
CGAAmTCGG
4
1599885
GATACTTGC
GATACTTGC
GATACTTGC
GATACTTGC
4
1850288
ATTCGTGTAATTCATGTAATTCGTGTAATTCrTGTA
4
2100356
TCAGAGACC
TCAGAGACC
TCAGGGACC
TCAGrGACC
4
2284257
TCTGGACTG
TCTGAACTG
TCTGAACTG
TCTGrACTG
5
100211
TCCTTGAATTCCTGGAAT
TCCTGGAAT
TCCTGGAAT
5
350872
GGCGTGCCC
GGCGTGCCC
GGCGTGCCC
GGCGyGCCC
5
599922
CGTCATTCA
CGTCATTCA
CGTCGTTCA
CGTCrTTCA
5
851262
TAATTCTCTTAATCGTCTTAATCGTCTTAATCGTCT
5
1099776
ACATTGACA
ACATCGACA
ACATTGACA
ACATyGACA
5
1352539
TTGTGATCCTTGTGATCCTTGTTGTCCTTGTkrTCC
5
1599904
AACTTCCTT AACTCCCTT AACTCCCTT AACTCCCTT
5
1851458
AAATAATCC
AAATTCTCC
AAATTCTCC
AAATTCTCC
5
2100025
CCCTTAGTCCCCTTAGTCCCCTCAGTC
CCCTyAGTC
5
2278878
GGTCAAAAA
GGTCAAAAA
AGTCAAAAA
rGTCAAAAA
6
106294
GCCATCTCG
GCCACCTCG
GCCATCTCG
GCCAyCTCG
6
350337
CATTTGGTTCATTCGGTTCATTTGGTTCATTyGGTT
6
600047
GGAGCATTT
GGAGTATTT
GGAGCATTT
GGAGyATTT
6
849985
AGTTCAGGA
AGTTCAGGA
AGTTCAGGA
AGTTCAGGA
6
1098535
CAAAGATTG
CAAAAATTG
CAAAGATTG
CAAArATTG
6
1349453
TGTCGGTAG
TGTCAATAG
TGTCGGTAG
TGTCrrTAG
6
1600000
AAACCTGGA
AAACCTGGA
AAACCTGGA
AAACCTGGA
6
1764645
AACCAGATT
AACCGGATT
AACCGGATT
AACCrGATT
6
2000087
GATTTTGCGGATTTTGCGGATTTTGCGGATTTTGCG
6
2252662
GGGTTGGTA
GGGTCGGTA
GGGTTGGTA
GGGTyGGTA
7
100284
GAAATTCAG
GAAACTCAG
GAAATTCAG
GAAAyTCAG
7
350044
ATATTCTTT ATATCCTTT ATATTCTTT ATATyCTTT
7
600111
CAATTATTACAATCATTA
CAATTATTA
CAATyATTA
7
850246
TGACGCATA
TGACGCATA
TGACGCATA
TGACGCATA
7
1100248
TCACGGAAG
TCACAGAAG
TCACGGAAG
TCACrGAAG
7
1350089
CTTTTCCCC CTTTTCCCC CTTTTCCCC CTTTTCCCC
7
1605047
ATACTTGGC
ATACTTGAC
ATACTTGGC
ATACTTGrC
7
1860993
GTCAACCGG
GTCATCCGG
GTCAACCGG
GTCAwCCGG
7
1898793
TCCGCATAA
TCCGCATAA
TCCGCATAA
TCCGCATAA
7
1991505
TCTACGGTTTCTACGGTTTCTACGGTTTCTACGGTT
8
350000
ATTGACGCG
ATTGGCGCG
ATTGGCGCG
ATTGGCGCG
8
610549
GAACTTGAT
GAATACGAT
GAATTTGAT
GATwyGAT
8
1100000
CATACGATC
CATACGATC
CATACGATC
CATACGATC
8
1100546
GCCCCAGAA
GCCCCAGAA
GCCCGAGAA
GCCCsAGAA
8
1350000
AGCTTAACA
AGCTTAACA
AGCTTAACA
AGCTTAACA
8
1350240
ACGGGTACT
ACGGATACT
ACGGGTACT
ACGGrTACT
8
1600100
CTGAACCCT
CTGAACCCT
CTGAACCCT
CTGAACCCT
9
100105
CTCAACCGA
CTCAACCGA
CTCAGCCGA
CTCArCCGA
9
352455
AGTCCTCCA
AGTCCTCCA
AGTCTCCCA
AGTCyyCCA
9
599950
TGGTATCCC
TGGTGTCCC
TGGTGTCCC
TGGTGTCCC
9
1010845
GGGTGGTGA
GGGTGGTGA
GGGTAGTGA
GGGTrGTGA
9
1244202
GATGAAGAT
GATGAAGAT
GATGGAGAT
GATGrAGAT
9
1504476
TACTGTACC
TACTGTACC
TACTATACC
TACTrTACC
9
1656962
TATCTACTGTATCTACTGTATCTACTGTATCTACTG
10
100438
AATTAATTTGATTAATTTAATTAATTTrATTAATTT
10
350030
GCGGCTCAA
GCGGTTCAA
GCGGTTCAA
GCGGTTCAA
10
600032
TTACACTGG
TTACACTGG
TTACACTGG
TTACACTGG
10
850000
TCGGTCGGA
TCGGTCGGA
TCGGTCGGA
TCGGTCGGA
10
860249
CCGCAAATT
CCGCGAATT
CCGCAAATT
CCGCrAATT
10
1173178
ATCCCCAAT
ATCCTCAAT
ATCCTCAAT
ATCCTCAAT
10
1303902
TGATTTACTTGATTTACTTGATTTACTTGATTTACT
10
1490452
AATCAGATG
AATCAGATG
AATCAGATG
AATCAGATG
11
101855
CCAGCCTGT
CCAGTCTGT
CCAGCCTGT
CCAGyCTGT
11
350000
GTCAGCAAG
GTCAGCAAG
GTCAGCAAG
GTCAGCAAG
11
350025
GATAAAACA
GATAAAACA
GATATAACA
GATAwAACA
11
600000
ATGGGCGCG
ATGGGAGCG
ATGGGAGCG
ATGGGrGCG
11
929659
GGAATATCA
GGAAGTTCA
GGAATATCA
GGAAkwTCA
11
1100608
AGTGGTCTTAGTGGTCTTAGTGATCTTAGTGrTCTT
11
1240230
ACAAGTTTC
ACAAATTTC
ACAAGTTTC
ACAArTTTC
12
100000
CCTTCTAGTCCTTCTAGTCCTTCTAGTCCTTCTAGT
12
109790
GTCTGCACC
GTCTACACC
GTCTGCACC
GTCTrCACC
12
1000000
CGAGGAGGA
CGAGGAGGA
CGAGAAGGA
CGAGrAGGA
13
100697
ACGTCTTTAACGTCTTTAACGTCTTTAACGTCTTTA
13
370521
TTTGAGTCATTTGTGTCATTTGTGTCATTTGTGTCA
13
604345
CTTCAGCAT
CTTCAGCAT
CTTCAGCAT
CTTCAGCAT
13
850249
GGCTAGTAA
GGCTAGTAA
GGCTAGTAA
GGCTAGTAA
14
113109
AGGGAAATA
AGGGGAATA
AGGGAAATA
AGGGrAATA
14
372086
CGATCCCTTCGATTCCTTCGATCCCTTCGATyCCTT
14
725684
ATGAGTTCG
ATGAGTTTG
ATGAATTCG
ATGArTTyG
15
150013
GTGGCCCGT
GTGGACCGT
GTGGCCCGT
GTGGmCCGT
15
449860
GAATTTCGG
GAATTTCGG
GAATTTCGG
GAATTTCGG
16
205778
CAAGGTCTG
CAAGATCTG
CAAGATCTG
CAAGATCTG
16
400000
CCTCGGATT
CCTCGGATT
CCTCGGATT
CCTCGGATT
16
403998
CCAAGTACG
CCAAATACG
CCAAGTACG
CCAArTACG
17
120000
TATTCTTCA TATTCTTCA TATTCTTCA TATTCTTCA
17
134676
TTGCCCAGC
TTGCTCAGC
TTGCCCAGC
TTGCyCAGC
17
338415
TGAGAAGCC
TGAGAAGCC
TGAGAAGCC
TGAGAAGCC
17
449833
ATCAGACAA
ATCAAACAA
ATCAAACAA
ATCAAACAA
18
101884
ATTACGGAC
ATTACGGAC
ATTACGGAC
ATTACGGAC
18
112940
GCGGGTGGG
GCGGCTGGG
GCGGGTGGG
GCGGsTGGG
19
98520
GCTATTGGG
GCTATTGGG
GCTATTGGG
GCTATTGGG
19
98782
AAAATTGTT
AAAAGTGTT
AAAATTGTT
AAAAkTGTT
and wherein Table II is provided as follows:
TABLE II
Markers:
Match
Strain:
p1n150/Mat
MFPC-1-ELF
FF
AN
AS
ITS
%
J15051
2/5
E3/E4
FF2/FF2
N3/N4
SA/SD
I1/I5
[100%]
B14528
2/5
E3/E4
FF1/FF3
N3/N4
SC/SD
I1/I2
66.7%
28c, [Heirloom]
1T/5
E3/E4
FF1/FF3
N2/N3
SA/SD
I1/I1
58.3%
28b, [Braun]
1T/5
E3/E4
FF1/FF4
N2N3/
SB/SD
I1/I1
50.0%
PTA-6877
1T/2
E2/E3
FF1/FF1
N2/N4
SD/SD
I1/I2
33.3%
S600
1T/2
E1/E3
FF2/FF2
N2/N4
SC/SD
I1/I2
41.7%
J10263/2
1T/2
E1/E3
FF2/FF7
N4/N7
SC/SD
I1/I2
50.0%
OFB lineage group
1T/3
E3/E6
FF1/FF2
N4/N4
SC/SD
I1/I3
41.7%
J14756-s3
2
E3
FF2
N4
SD
I5
wild line
5
E4
FF2
N3
SA
I1,
from the subsequent hybrid of step (d).
12. The Agaricus bisporus culture of claim 2 , wherein said F1 hybrid mushroom culture is a culture of strain J15051, a culture of the strain J15051 having been deposited under the NRRL accession number 67316.
13. The Agaricus bisporus culture of claim 2 , wherein said F1 hybrid mushroom culture produces, when grown under the same commercial conditions with at least one dark-brown strain selected from the group consisting of BR06/“Heirloom” and B14528/“Tuscan,” a crop of mushrooms (1) having a cap color that is a lighter brown color than at least one of the dark-brown strains selected from the group, and wherein (2) the crop is produced not later than crops produced by at least one of the dark-brown strains selected from the group, and wherein (3) the crop yield is at least 95% of the yield of at least one of the dark-brown strains selected from the group.
14. An Essentially Derived Variety culture from an initial culture which is the culture of claim 12 , wherein the Essentially Derived Variety culture has at least 75% genotypic and genomic identity with the culture of strain J15051.
15. A part of the culture of claim 2 selected from the group consisting of hyphae and cells.
16. A part of the culture of claim 12 selected from the group consisting of hyphae, spores, cells, and nuclei.
17. A mushroom produced by growing a crop of mushrooms from the culture of claim 1 .
18. A mushroom produced by growing a crop of mushrooms from the F1 hybrid Agaricus bisporus mushroom culture of claim 2 .
19. A process of producing a hybrid mushroom culture, comprising:
physically mating a first Agaricus bisporus mushroom culture with a second Agaricus bisporus mushroom culture, wherein at least one of the first and second mushroom cultures is a culture having all of the physiological and morphological characteristics of line J14756-s3, wherein a culture of said line J14756-s3 having been deposited under the NRRL Accession Number 67317.
20. A hybrid culture produced by the process of claim 19 .
21. A part of the hybrid culture of claim 20 , selected from the group consisting of hyphae and cells.
22. A hybrid mushroom, or its pieces or parts, produced by growing a crop of mushrooms from said hybrid culture of claim 20 .
23. A product incorporating the hybrid culture of claim 20 , the product selected from the group consisting of mycelium, spawn, inoculum, casing inoculum, fresh mushrooms, processed mushrooms, mushroom pieces, colonized substrates, grain, compost, and friable particulate matter.
24. A method of mushroom strain development comprising the steps of:
(a) obtaining a molecular marker profile of Agaricus bisporus mushroom line J14756-s3, a culture of said line having been deposited under the NRRL Accession Number 67317;
(b) obtaining an F n hybrid culture for which the mushroom culture of claim 1 is a parent, wherein n is an integer 1 to 10;
(c) mating a culture obtained from the F n hybrid culture with a different mushroom culture to produce an F (n+1) hybrid culture; and
(d) selecting progeny of the F (n+1) hybrid culture that possess characteristics of said molecular marker profile of line J14756-s3.
25. An Agaricus bisporus culture having all of the physiological and morphological characteristics of line J14756-s3, wherein a culture of said line J14756-s3 having been deposited under the NRRL Accession Number 67317.
26. The Agaricus bisporus culture of claim 25 , further comprising a marker profile in accordance with the marker profile of line J14756-s3 shown in Table I, wherein Table is provided as follows:
TABLE I
Scaffold
Position of SNP
Cultures:
[H97 V2.0 ref. coordinates]
H97
Wild Line
J14756-s3
J15051
1
99995
CTACATTGA
CTACGTTGA
CTACGTTGA
CTACGTTGA
1
349966
AAGGTGGTT
AAGGCGGTT
AAGGCGGTT
AAGGCGGTT
1
600059
TTTTTTTT- TTTTTTTTC TTTTCTTT- TTTTyTTTC
1
850014
CCTTTTCAC TCTTGTCACTCTTGTCACTCTTGTCAC
1
1099971
GTCGACACC
GTCGACACC
GTCGGCACC
GTCGrCACC
1
1350278
GGAGAGTCG
GGAGAGTCG
GGAGGTTCG
GGAGrkTCG
1
1599956
AATAAGCGC
AATAAGCGC
AATAGGCGC
AATarGCGC
1
1850032
CGAGTAATT
CGAGCAATT
CGAGCAATT
CGAGCAATT
1
2119049
ACAATCCAA
ACAACTCAA
ACAACTCAA
ACAACTCAA
1
2400243
ACTTCATGA
ACTTGATGA
ACTTGATGA
ACTTGATGA
1
2612870
AATAGGAGT
AATAGGAGT
AATAAGAGT
AATArGAGT
1
2858975
GCCGTTCTTGCCGCTCTTGCCGCTCTTGCCGCTCTT
1
2804522
GAAGACGAC
GAAGGCGAC
GAAGGCGAC
GAAGGCGAC
1
3047987
AAGGGGGGG
AAGGAGGGG
AAGGGGGGG
AAGGrGGGG
1
3212182
CACTACGTG
CACTGCGTG
CACTACGTG
CACTrCGTG
1
3256057
TATCTGTTT TATCCGTTT TATCCGTTT TATCCGTTT
2
101820
ATTAAAGAT
ATTAAAGAT
ATTAAAGAT
ATTAAAGAT
2
350156
TCGGGGGTG
TCGGAGGTG
TCGGGGGTG
TCGGrGGTG
2
600112
ATGTATACG
ATGTGTACG
ATGTATACG
ATGTrTACG
2
850338
TGGTGCTAA
TGGTTCTAATGGTGCTAA
TGGTkCTAA
2
1099413
CCTGACTCA
CCTGGCTCA
CCTGACTCA
CCTGrCTCA
2
1349512
CTCAGCAGT
CTCAACAGT
CTCAGCAGT
CTCArCAGT
2
1600085
CACAATGCC
CACATTGCC
CACAATGCC
CACAwTGCC
2
1901773
ACTCGAATT
ACTAAAATT
ACTCAAATT
ACTmAAATT
2
2150201
GTCGTAGGT
GTCGAAGGT
GTCGTAGGT
GTCGwAGGT
2
2400281
TCAAAACCC
TCAACACCC
TCAACACCC
TCAACACCC
2
2650136
ATAATTCCTATAAGTCCT
ATAATTCCT
ATAAkTCCT
2
2903620
ACTAAAAGA
ACTAAAAGA
ACTAAAAGA
ACTAAAAGA
2
3048019
GTCCGCTGC
GTCCGCTGC
GTCCGCTGC
GTCCGCTGC
3
57117
TATAGCAGT
TATGACAGT
TATGACAGT
TATGACAGT
3
65650
GGCGCTTTTGGCGGTTTTGGCGGTTTTGGCGGTTTT
3
119181
TTTATACTCTTTATACTCTTTATACTCTTTATACTC
3
250112
GCAGGAGAG
GCCGAAGAG
GCAGGAGAG
GCmGrAGAG
3
750000
GTCCGGCCA
GTCCGGCCA
GTCCGGCCA
GTCCGGCCA
3
750074
AGTTTTTTC AGTTATTTC AGTTTTTTC AGTTwTTTC
3
1250000
TTTTTCCGG TTTTTCCGG TTTTTCCGG TTTTTCCGG
3
1250161
AGTCTCCTT AGTCCCCTT AGTCTCCTT AGTCyCCTT
3
1750000
ACGCCTGAC
ACGCCTGAC
ACGCCTGAC
ACGCCTGAC
3
1750033
TTTTTTGCT TTTTGTGCT TTTTTTGCT TTTTkTGCT
3
2250000
CGTGGCGAT
CGTGGCGAT
CGTGGCGAT
CGTGGCGAT
3
2250027
TGTATCAAG
TGTGCCAAG
TGTATCAAG
TGTryCAAG
3
2520748
TAATTCCAC
TAATGCCAC
TAATTCCAC
TAATkCCAC
4
100004
GAGTGATAA
GAGTGATAA
GAGTGATAA
GAGTGATAA
4
490648
CGATCGCGT
CGATTGCGT
CGATCGCGT
CGATyGCGT
4
598147
GATCGACAG
GATCAACAG
GATCGACAG
GATCrACAG
4
852119
CGAATATTC
CGAATATTC
CGAATATTC
CGAATATTC
4
1100085
GATGCCGAA
GATGACGAA
GATGCCGAA
GATGmCGAA
4
1350536
CGAACTCGG
CGAAATCGG
CGAACTCGG
CGAAmTCGG
4
1599885
GATACTTGC
GATACTTGC
GATACTTGC
GATACTTGC
4
1850288
ATTCGTGTAATTCATGTAATTCGTGTAATTCrTGTA
4
2100356
TCAGAGACC
TCAGAGACC
TCAGGGACC
TCAGrGACC
4
2284257
TCTGGACTG
TCTGAACTG
TCTGAACTG
TCTGrACTG
5
100211
TCCTTGAATTCCTGGAAT
TCCTGGAAT
TCCTGGAAT
5
350872
GGCGTGCCC
GGCGTGCCC
GGCGCGCCC
GGCGyGCCC
5
599922
CGTCATTCA
CGTCATTCA
CGTCGTTCA
CGTCrTTCA
5
851262
TAATTCTCTTAATCGTCTTAATCGTCTTAATCGTCT
5
1099776
ACATTGACA
ACATCGACA
ACATTGACA
ACATyGACA
5
1352539
TTGTGATCCTTGTGATCCTTGTTGTCCTTGTkrTCC
5
1599904
AACTTCCTT AACTCCCTT AACTCCCTT AACTCCCTT
5
1851458
AAATAATCC
AAATTCTCC
AAATTCTCC
AAATTCTCC
5
2100025
CCCTTAGTCCCCTTAGTCCCCTCAGTC
CCCTyAGTC
5
2278878
GGTCAAAAA
GGTCAAAAA
AGTCAAAAA
rGTCAAAAA
6
106294
GCCATCTCG
GCCACCTCG
GCCATCTCG
GCCAyCTCG
6
350337
CATTTGGTTCATTCGGTTCATTTGGTTCATTyGGTT
6
600047
GGAGCATTT
GGAGTATTT
GGAGCATTT
GGAGyATTT
6
849985
AGTTCAGGA
AGTTCAGGA
AGTTCAGGA
AGTTCAGGA
6
1098535
CAAAGATTG
CAAAAATTG
CAAAGATTG
CAAArATTG
6
1349453
TGTCGGTAG
TGTCAATAG
TGTCGGTAG
TGTCrrTAG
6
1600000
AAACCTGGA
AAACCTGGA
AAACCTGGA
AAACCTGGA
6
1764645
AACCAGATT
AACCGGATT
AACCGGATT
AACCrGATT
6
2000087
GATTTTGCGGATTTTGCGGATTTTGCGGATTTTGCG
6
2252662
GGGTTGGTA
GGGTCGGTA
GGGTTGGTA
GGGTyGGTA
7
100284
GAAATTCAG
GAAACTCAG
GAAATTCAG
GAAAyTCAG
7
350044
ATATTCTTT ATATCCTTT ATATTCTTT ATATyCTTT
7
600111
CAATTATTACAATCATTA
CAATTATTA
CAATyATTA
7
850246
TGACGCATA
TGACGCATA
TGACGCATA
TGACGCATA
7
1100248
TCACGGAAG
TCACAGAAG
TCACGGAAG
TCACrGAAG
7
1350089
CTTTTCCCC CTTTTCCCC CTTTTCCCC CTTTTCCCC
7
1605047
ATACTTGGC
ATACTTGAC
ATACTTGGC
ATACTTGrC
7
1860993
GTCAACCGG
GTCATCCGG
GTCAACCGG
GTCAwCCGG
7
1898793
TCCGCATAA
TCCGCATAA
TCCGCATAA
TCCGCATAA
7
1991505
TCTACGGTTTCTACGGTTTCTACGGTTTCTACGGTT
8
350000
ATTGACGCG
ATTGGCGCG
ATTGGCGCG
ATTGGCGCG
8
610549
GAACTTGAT
GAATACGAT
GAATTTGAT
GATwyGAT
8
1100000
CATACGATC
CATACGATC
CATACGATC
CATACGATC
8
1100546
GCCCCAGAA
GCCCCAGAA
GCCCGAGAA
GCCCsAGAA
8
1350000
AGCTTAACA
AGCTTAACA
AGCTTAACA
AGCTTAACA
8
1350240
ACGGGTACT
ACGGATACT
ACGGGTACT
ACGGrTACT
8
1600100
CTGAACCCT
CTGAACCCT
CTGAACCCT
CTGAACCCT
9
100105
CTCAACCGA
CTCAACCGA
CTCAGCCGA
CTCArCCGA
9
352455
AGTCCTCCA
AGTCCTCCA
AGTCTCCCA
AGTCyyCCA
9
599950
TGGTATCCC
TGGTGTCCC
TGGTGTCCC
TGGTGTCCC
9
1010845
GGGTGGTGA
GGGTGGTGA
GGGTAGTGA
GGGTrGTGA
9
1244202
GATGAAGAT
GATGAAGAT
GATGGAGAT
GATGrAGAT
9
1504476
TACTGTACC
TACTGTACC
TACTATACC
TACTrTACC
9
1656962
TATCTACTGTATCTACTGTATCTACTGTATCTACTG
10
100438
AATTAATTTGATTAATTTAATTAATTTrATTAATTT
10
350030
GCGGCTCAA
GCGGTTCAA
GCGGTTCAA
GCGGTTCAA
10
600032
TTACACTGG
TTACACTGG
TTACACTGG
TTACACTGG
10
850000
TCGGTCGGA
TCGGTCGGA
TCGGTCGGA
TCGGTCGGA
10
860249
CCGCAAATT
CCGCGAATT
CCGCAAATT
CCGCrAATT
10
1173178
ATCCCCAAT
ATCCTCAAT
ATCCTCAAT
ATCCTCAAT
10
1303902
TGATTTACTTGATTTACTTGATTTACTTGATTTACT
10
1490452
AATCAGATG
AATCAGATG
AATCAGATG
AATCAGATG
11
101855
CCAGCCTGT
CCAGTCTGT
CCAGCCTGT
CCAGyCTGT
11
350000
GTCAGCAAG
GTCAGCAAG
GTCAGCAAG
GTCAGCAAG
11
350025
GATAAAACA
GATAAAACA
GATATAACA
GATAwAACA
11
600000
ATGGGCGCG
ATGGGAGCG
ATGGGAGCG
ATGGGrGCG
11
929659
GGAATATCA
GGAAGTTCA
GGAATATCA
GGAAkwTCA
11
1100608
AGTGGTCTTAGTGGTCTTAGTGATCTTAGTGrTCTT
11
1240230
ACAAGTTTC
ACAAATTTC
ACAAGTTTC
ACAArTTTC
12
100000
CCTTCTAGTCCTTCTAGTCCTTCTAGTCCTTCTAGT
12
109790
GTCTGCACC
GTCTACACC
GTCTGCACC
GTCTrCACC
12
1000000
CGAGGAGGA
CGAGGAGGA
CGAGAAGGA
CGAGrAGGA
13
100697
ACGTCTTTAACGTCTTTAACGTCTTTAACGTCTTTA
13
370521
TTTGAGTCATTTGTGTCATTTGTGTCATTTGTGTCA
13
604345
CTTCAGCAT
CTTCAGCAT
CTTCAGCAT
CTTCAGCAT
13
850249
GGCTAGTAA
GGCTAGTAA
GGCTAGTAA
GGCTAGTAA
14
113109
AGGGAAATA
AGGGGAATA
AGGGAAATA
AGGGrAATA
14
372086
CGATCCCTTCGATTCCTTCGATCCCTTCGATyCCTT
14
725684
ATGAGTTCG
ATGAGTTTG
ATGAATTCG
ATGArTTyG
15
150013
GTGGCCCGT
GTGGACCGT
GTGGCCCGT
GTGGmCCGT
15
449860
GAATTTCGG
GAATTTCGG
GAATTTCGG
GAATTTCGG
16
205778
CAAGGTCTG
CAAGATCTG
CAAGATCTG
CAAGATCTG
16
400000
CCTCGGATT
CCTCGGATT
CCTCGGATT
CCTCGGATT
16
403998
CCAAGTACG
CCAAATACG
CCAAGTACG
CCAArTACG
17
120000
TATTCTTCA TATTCTTCA TATTCTTCA TATTCTTCA
17
134676
TTGCCCAGC
TTGCTCAGC
TTGCCCAGC
TTGCyCAGC
17
338415
TGAGAAGCC
TGAGAAGCC
TGAGAAGCC
TGAGAAGCC
17
449833
ATCAGACAA
ATCAAACAA
ATCAAACAA
ATCAAACAA
18
101884
ATTACGGAC
ATTACGGAC
ATTACGGAC
ATTACGGAC
18
112940
GCGGGTGGG
GCGGCTGGG
GCGGGTGGG
GCGGsTGGG
19
98520
GCTATTGGG
GCTATTGGG
GCTATTGGG
GCTATTGGG
19
98782
AAAATTGTT
AAAAGTGTT
AAAATTGTT
AAAAkTGTT.
27. An Essentially Derived Variety culture derived from an initial culture which is the culture of claim 2 , wherein the Essentially Derived Variety culture has at least 75% genotypic and genomic identity with the culture of the F1 hybrid mushroom culture produced by mating a culture of the line J14756-s3 with a different Agaricus bisporus culture.