Patent
Disclosed herein is one or more subtilisin variant. nucleic acid encoding same. and compositions and methods related to the production and use thereof. including one or more Bacillus gibsonii -clade subtilisin variant that has improved soil removal compared to one or more reference subtilisin.
1. A subtilisin variant comprising an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in SEQ ID NO: 1, wherein the variant has the substitutions 5039E-5099R-S126A-D127E-F128G numbered by correspondence with the amino acid sequence of SEQ ID NO:1, and wherein the variant has a blood/milk/ink on cotton (BMI), egg, and/or crème brûlée stain cleaning PI>1 when compared to SEQ ID NO:1.
2. The subtilisin variant of claim 1 , wherein the variant has combinations of mutations selected from the group consisting of:
S039E-S099R-S126A-D127E-F128G-M211L, 5039E-S099R-S126A-D127E-F128G-N242D, 5039E-S099R-S126A-D127E-F128G-N253D, 5039E-S099R-S126A-D127E-F128G-Q200L, 5039E-S099R-S126A-D127E-F128G-Q256E, 5039E-S099R-S126A-D127E-F128G-S255W, 5039E-5099R-S126A-D127E-F128G-V199I, S039E-5099R-S126A-D127E-F128G-Y203W, 5039E-5099R-T1140-S126A-D127E-F128G, 5039E-T056Y-N074D-S099R-S126A-D127E-F128G, 5039E-T056Y-S099R-S126A-D127E-F128G, 5039E-T056Y-S099R-S126A-D127E-F128G-M211L, and T009E-5039E-5099R-S126A-D127E-F128G, numbered by correspondence with the amino acid sequence of SEQ ID NO:1.
3. The subtilisin variant of claim 1 , wherein the variant comprises an amino acid sequence with 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or less than 100% amino acid sequence identity to the amino acid sequence of SEQ ID NO:1.
4. A composition comprising the substilisin variant of claim 1 .
5. The composition of claim 4 , wherein the composition is selected from an enzyme composition and a detergent composition.
6. The composition of claim 5 , wherein the detergent composition is selected from a laundry detergent, a fabric softening detergent, a dishwashing detergent, and a hard-surface cleaning detergent.
7. The composition of claim 4 , further comprising (i) one or more enzymes selected from acyl transferases, amylases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinases, arabinosidases, aryl esterases, beta-galactosidases, beta-glucanases, carrageenases, catalases, chondroitinases, cutinases, endo-beta-mannanases, exo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, lactases, ligninases, lipases, lipolytic enzymes, lipoxygenases, mannanases, metalloproteases, oxidases, oxidoreductases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, perhydrolases, peroxidases, phenoloxidases, phosphatases, phospholipases, polyesterases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, cellulases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, and xylosidases; (ii) one or more surfactants; (iii) one or more ions selected from calcium and zinc; (iv) one or more adjunct material; (v) one or more stabilizers; (vi) one or more bleaching agents and/or (vii) combinations thereof.
8. The composition of claim 4 , wherein the composition is phosphate-free or contains phosphate and/or is boron free or contains boron.
9. The composition of claim 4 , wherein the composition is granular, powder, solid, bar, liquid, tablet, gel, paste and/or a unit dose composition.