PROCESS FOR PRODUCING A FERMENTED MILK PRODUCT
The present invention relates to a process for producing a fermented milk product comprising the steps of 1) adding a starter culture comprising at least one lactic acid bacteria strain to a milk base, 2) fermenting the milk for a period of time until a target pH is reached, 3) using a starter culture comprising at least one lactose-deficient strain, which is capable of metabolizing a non-lactose carbohydrate, and 4) adding a low pH stable lactase to the process either at the start, during or at the end of the fermentation step, wherein the low pH stable lactase retains its activity at a pH of 5.0 and a temperature of 37° C. at a level of at least 5% as compared to its activity at the optimum pH of the lactase.
1 - 15 . (canceled)
16 . A process for producing a fermented milk product comprising:
(a) adding a starter culture comprising lactic acid bacteria to a milk base, wherein the starter culture comprises at least one lactose-deficient lactic acid bacteria strain that is capable of metabolizing a non-lactose carbohydrate,
(b) fermenting the milk for a period of time until a target pH is reached, and
(c) adding a low pH stable lactase to the milk base at the start, during, or at the end of the fermenting step, wherein the low pH stable lactase retains at least 5% of its activity at a pH of 5.0 and a temperature of 37° C. as compared to its activity at its optimum pH, thereby obtaining a fermented milk product.
17 . A process according to claim 16 , wherein the low pH stable lactase retains at least 10% of its activity at a temperature of 10° C. and a pH of 6.0 as compared to its activity at its optimum temperature.
18 . A process according to claim 16 , wherein the lactose-deficient strain is capable of metabolizing a non-lactose carbohydrate selected from sucrose, galactose and glucose.
19 . A process according to claim 16 , wherein a non-lactose carbohydrate is added to the milk base at the start of the fermenting step.
20 . A process according to claim 19 , wherein the non-lactose carbohydrate is added in an amount determined to become depleted and result in stopping growth of the lactic acid bacteria and stopping the fermentation at the target pH.
21 . A process according to claim 16 , wherein the low pH stable lactase is added to the milk base at the start of the fermenting step.
22 . A process according to claim 21 , wherein no non-lactose carbohydrate is added at the start of or during the fermenting step, and wherein at least one lactose-deficient lactic acid strain of the starter culture is capable of metabolizing a carbohydrate selected from glucose and galactose.
23 . A process according to claim 16 , wherein the low pH stable lactase is added to the milk base at the end of the fermenting step.
24 . A process according to claim 16 , wherein the lactose-deficient strain is selected from lactose-deficient Streptococcus thermophilus and lactose-deficient Lactobacillus delbrueckii subsp. bulgaricus.
25 . A process according to claim 24 , wherein the lactose-deficient strain is selected from:
(a) a Streptococcus thermophilus strain selected from (i) the strain deposited with Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ) under accession number DSM 28952 and (ii) strains derived from DSM 28952, wherein the derived strain has the ability to generate white colonies on a medium containing lactose and X-Gal;
(b) a Streptococcus thermophilus strain selected from (i) the strain deposited with DSMZ under accession number DSM 28953 and (ii) strains derived from DSM 28953, wherein the derived strain has the ability to generate white colonies on a medium containing lactose and X-Gal; and
(c) a Lactobacillus delbrueckii ssp. bulgaricus strain selected from (i) the strain deposited with DSMZ under accession number DSM 28910 and (ii) strains derived from DSM 28910, wherein the derived strain has the ability to generate white colonies on a medium containing lactose and X-Gal.
26 . A process according to claim 24 , wherein the starter culture contains both at least one lactose-deficient Streptococcus thermophilus strain and at least one lactose-deficient Lactobacillus delbrueckii subsp. Bulgaricus strain.
27 . A process according to claim 24 , wherein the starter culture contains both at least one lactose-deficient Streptococcus thermophilus strain and at least one lactose-deficient Lactobacillus delbrueckii subsp. Bulgaricus strain, wherein all Streptococcus thermophilus strains and all Lactobacillus delbrueckii subsp. bulgaricus strains present are lactose-deficient.
28 . A process according to claim 16 , wherein the low pH stable lactase comprises an amino acid sequence at least 90% identical to an amino acid sequence selected from the amino acid sequence of residues 28-1931 of SEQ ID NO:1, the amino acid sequence of residues 28-1331 of SEQ ID NO:2, the amino acid sequence of SEQ ID NO:3, and the amino acid sequence of SEQ ID NO:4, and lactase active fragments thereof.
29 . A process according to claim 16 , wherein the low pH stable lactase comprises an amino acid sequence selected from the amino acid sequence of residues 28-1931 of SEQ ID NO:1, residues 28-1331 of SEQ ID NO: 2, SEQ ID NO: 3, and SEQ ID NO: 4.
30 . A process according to claim 16 , wherein the obtained fermented milk product has increased texture as compared to a fermented milk product obtained by the same method but without adding a low pH stable lactase.
31 . A process according to claim 16 , wherein the obtained fermented milk product has increased texture as compared to a fermented milk product obtained by the same method but without using lactose-deficient lactic acid bacteria.
32 . A fermented milk product produced by the process of claim 16 .