PROTEINIC BIOMASS PREPARATION COMPRISING A NON-NATIVE ORGANISM OF THE CLOSTRIDIA CLASS

1 . A proteinic biomass preparation comprising a non-native organism of the Clostridia class, which organism expresses

(i) a modified aspartate kinase characterized by reduced lysine inhibition, reduced threonine inhibition, and/or reduced methionine inhibition compared with the unmodified enzyme in native organism of the same genus and species;

(ii) a modified homoserine dehydrogenase characterized by reduced threonine inhibition compared with the unmodified enzyme in native organism of the same genus and species;

(iii) a modified homoserine kinase characterized by reduced methionine inhibition compared with the unmodified enzyme in native organism of the same genus and species;

(iv) a modified anthranilate synthase characterized by reduced tryptophan inhibition compared with the unmodified enzyme in native organism of the same genus and species;

(v) a functional lycopene pathway and the genes crtY, crtW, and crtZ and/or

(vi) a functional oleic acid pathway and the four gene operon (pfaABCD).

2 - 15 . (canceled)

16 . A preparation according to claim 1 , wherein said non-native organism is an acetogen.

17 - 18 . (canceled)

19 . A preparation according to claim 1 , comprising, on a dry basis, at least 55% wt protein.

20 . A preparation according to claim 1 , comprising at least one selected from the group consisting of:

on total protein content, at least 6% wt lysine;

(ii) on total protein content, at least 3% wt threonine;

(iii) on total protein content, at least 1.5% wt methionine;

(iv) on total protein content, at least 0.5% wt tryptophan;

(v) on a dry basis, at least 0.01% wt astaxanthin;

(vi) on a dry basis, at least 0.1% wt eicosapentaenoic acid; and

(vii) on a dry basis, at least 0.1% wt docosahexaenoic acid.

21 - 43 . (canceled)

44 . A proteinic biomass preparation comprising an organism of the Clostridia class, wherein said preparation comprises,

(i) on dry basis at least 55% wt protein;

(ii) on total protein content, at least 6% wt lysine;

(iii) on total protein content, at least 3% wt threonine;

(iv) on total protein content, at least 1.5% wt methionine;

(v) on total on total protein content, at least 0.5% wt tryptophan;

(vi) on a dry basis, at least 0.01% wt astaxanthin;

(vii) on a dry basis, at least 0.1wt eicosapentaenoic acid, and/or

(viii) on a dry basis, at least 0.1% wt docosahexaenoic acid.

45 . (canceled)

46 . A preparation according to claim 44 , comprising (i) and at least one of (ii) to (v).

47 - 50 . (canceled)

51 . A preparation according to claim 44 , wherein said organism expresses

(i) a modified aspartate kinase characterized by reduced lysine inhibition, reduced threonine inhibition, and/or reduced methionine inhibition compared with the unmodified enzyme in native organism of the same genus and species;

(ii) a modified homoserine dehydrogenase characterized by reduced threonine inhibition compared with the unmodified enzyme in native organism of the same genus and species;

(iii) a modified homoserine kinase characterized by reduced methionine inhibition compared with the unmodified enzyme in native organism of the same genus and species;

(iv) a modified anthranilate synthase characterized by reduced tryptophan inhibition compared with the unmodified enzyme in native organism of the same genus and species;

(v) a functional lycopene pathway and the genes crtY, crtW, and crtZ and/or

(vi) a functional oleic acid pathway and the four gene operon (pfaABCD)

52 - 55 . (canceled)

56 . A biomass according to claim 44 , wherein said organism further expresses the gene pfaE.

57 . A preparation according to claim 44 , wherein said organism is selected from Butyribacterium methylotrophicum, Eubacterium limosum , and Clostridium kluyveri.

58 . A preparation according to claim 44 , wherein said organism is an acetogen.

59 - 61 . (canceled)

62 . A preparation according to claim 44 , further comprising digestibility-enhancing enzymes selected from the group consisting of phytases, cellulases, lipases, amylases, arabinases, pectinases, mannases, keratinases, proteases, tannases, galactosidases, glucosidases, invertases and combinations thereof.

63 . (canceled)

64 . A preparation according to claim 51 , wherein said non-native organism further expresses a diphosphate-fructose-6-phosphate 1-phosphotransferase (PFP, EC 2.7.1.90).

65 . A preparation according to claim 44 , wherein phosphofructokinase 1 (EC 2.7.1.11, pfkA, BUME_09340) has been deleted from the genome of said non-native organism.

66 . A preparation according to claim 44 , wherein acetyl-CoA acetyltransferase gene (th1A, EC 2.3.1.9, BUME_07140) been deleted from the genome of said non-native organism.

67 . Animal feed comprising a preparation according to claim 44 .

68 . (canceled)

69 . A method for producing of a biomass comprising culturing an organism according to claim 44 in a fermentation medium comprising a carbon source and a nitrogen source, whereby biomass is generated in a fermentation broth.

70 . A method according to claim 69 , wherein said culturing is anaerobic.

71 - 73 . (canceled)

74 . A method according to claim 69 , wherein said non-native organism fixes CO 2 .

75 . (canceled)

76 . A method according to claim 69 , wherein biomass generation yield is greater than 35 gram biomass per 100 gram of carbon source consumed.