Packing material for size exclusion chromatography and method for producing the same
An object of the present invention is to provide a packing material suitable for use as a packing material for size exclusion chromatography for fractionation that requires large-scale treatment, the packing material being capable of being produced by a simple process and reducing column pressure drop even when the particle diameter is small, and is to provide a method for producing the packing material. In the present invention, a packing material for size exclusion chromatography is obtained by a production process including polymerizing glycerol 1,3-dimethacrylate and glycidyl methacrylate in the presence of a polymerization initiator, hydrophilizing the resulting porous particles made of a copolymer using a sugar alcohol, and then opening the rings of remaining glycidyl groups using a mineral acid.
1. A packing material for size exclusion chromatography, comprising porous particles comprising copolymer particles comprising at least glycerol 1,3-dimethacrylate and glycidyl methacrylate as monomer units and a structure represented by the following formula (I) on a surface of the copolymer particle:
[Chem. 1]
R 1 —O—CH 2 —[CH(OH)] n -CH 2 —O—R 2 (I)
wherein R 1 represents a partial structure of the porous particles, R 2 represents a hydrogen atom, and n represents 4 or 5,
wherein the partial structure has a glycidyl group that reacts with a primary hydroxyl group of a sugar alcohol to form an ether linkage with the porous particle, and
wherein a ring-opening rate of glycidyl groups of the porous particles is 95% or more.
2. The packing material for size exclusion chromatography according to claim 1 , wherein the copolymer comprises 6 to 29 mol % of glycerol 1,3-dimethacrylate and 71 to 94 mol % of glycidyl methacrylate as the monomer units.
3. The packing material for size exclusion chromatography according to claim 1 , wherein n in the formula (I) is 4.
4. A column for size exclusion chromatography, comprising a housing for liquid chromatography and the packing material according to claim 1 packed into the housing for liquid chromatography.
5. A chromatography apparatus comprising the column for size exclusion chromatography according to claim 4 .
6. A method for separating and franctionating biopolymers, comprising separating the biopolymers with an aqueous eluent using the column for size exclusion chromatography according to claim 4 .