Granted Patent B2

US 12,275,954 · App. 16/488,380 · Granted Apr 15, 2025

Inventors: Jane Sowden (London, GB); Jorn Lakowski (London, GB); Robin Ali (London, GB); Emily Welby (London, GB); Anai Gonzalez Cordero (London, GB)

Assignee: UCL BUSINESS Ltd.

C12N5/062A61K9/0048A61K35/30G01N33/56966G01N33/68C12N2506/02C12N2506/45

Abstract

Claims (7)

1. A method of identifying L/M-Opsin and CRX positive cone photoreceptor cells in a population of cells, comprising: a) detecting and determining whether or not cells in the population express CD29 or CD15-SSEA1 on cell surface; b) detecting and determining whether or not cells in the population express at least two of CD26, CD133 and CD 147 on the cell surface; and c) identifying cells as L/M-Opsin and CRX positive cone photoreceptor cells based on presence of L/M-Opsin and CRX, absence of CD29 or CD15-SSEA1, and presence of at least two of CD26, CD133 and CD147, and wherein the cells are L/M-Opsin and CRX positive, CD29 or CD15-SSEA1 negative and positive for at least two of CD26, CD133 and CD147.

2. The method of claim 1 , wherein the cells are L/M-Opsin and CRX positive, CD15-SSEA1 negative and positive for at least two of CD26, CD133 and CD147.

3. The method of claim 2 , wherein the cells are L/M-Opsin and CRX positive, CD 15-SSEA1 negative and positive for CD26, CD133 and CD147.

4. The method of claim 1 , wherein the population of cells are cultured and yielded from: a) human induced pluripotent stem cells or cells derived from human induced pluripotent stem cells; b) human embryonic stem cells or cells derived from human embryonic stem cells; c) human fetal retinal cells or cells derived from human fetal retinal cells; or d) directly converted human somatic cell populations or cells derived from directly converted human somatic cell populations.

5. The method of claim 1 , further comprising culturing the population of cells prior to the identification steps, to allow for differentiation of cells to the L/M-Opsin and CRX positive cone photoreceptor populations.

6. The method of claim 1 , further comprising a step of isolating the identified L/M-Opsin and CRX positive cone photoreceptor cells from the population of cells by magnetic-activated cell sorting (MACS) or fluorescence-activated cell sorting (FACS).

7. The method of claim 6 , further comprising a step of culturing the isolated identified cells to allow the cells to differentiate into mature L/M-Opsin and CRX positive cone photoreceptors.

Assignments (2)

Priority Claims (1)

Continuity (1)

References Cited (69)