Process for concentrating cells from a sample and then isolating nucleic acids from said cells
Biological cells are concentrated and isolated from a sample and/or biological cells are concentrated from a sample, followed by the isolation of nucleic acids from said cells. The sample is brought into contact with a solid phase which has a rough or structured surface.
1. A method for concentrating and isolating biological cells from a sample, comprising:
contacting a solid phase having a rough and/or structured surface with the sample by placing the solid phase into a cavity containing the sample;
agitating and/or aspirating the sample with the solid phase having the rough and/or structured surface to adsorb the biological cells onto the solid phase.
2. The method according to claim 1 , further comprising:
adding a salt of a divalent or trivalent cation to the sample prior to the contacting.
3. The method according to claim 2 , wherein the salt is calcium chloride.
4. The method according to claim 1 , wherein the rough and/or structured surface is a non-smooth plastic surface or rubber surface.
5. The method according to claim 4 , wherein the non-smooth plastic surface is created by a 3D print or by roughening of a plastic material.
6. The method according to claim 1 , wherein the solid phase comprises a rough composite material.
7. The method according to claim 1 , wherein the solid phase comprises a roughened pipette tip.
8. The method according to claim 7 , wherein the roughened pipette tip has a magnetic or paramagnetic property.
9. The method according to claim 1 , wherein the solid phase with the rough and/or structured surface is introduced into or put onto a smooth hollow body.
10. The method according to claim 9 , wherein the solid phase with the rough and/or structured surface is a ring or a sleeve and the smooth hollow body is a pipette tip.
11. A method for concentrating biological cells from a sample and for subsequently isolating nucleic acid from the biological cells, comprising:
a) contacting a solid phase having a rough and/or structured surface with the sample by placing the solid phase into a cavity containing the sample to adsorb the biological cells onto the solid phase,
b) lysing the biological cells,
c) binding the nucleic acid from the biological cells released by the lysing to the solid phase,
d) optionally, washing and subsequently drying the solid phase, and
e) finally detaching the nucleic acid from the solid phase with an elution buffer.
12. The method according to claim 11 , wherein the method is carried out:
a. manually or fully automatically as a single tube process, or
b. as an automated walk-away method.
13. The method according to claim 11 , wherein the elution buffer is a low salt buffer or water.