Apparatus and methods for irradiating organ perfusates
Disclosed are apparatuses and methods for irradiating a perfusate. The apparatus includes a tank which defines a first chamber. A separator is located inside the first chamber. The separator defines a second chamber. The first chamber and the second chamber are concentric and have substantially annular cross sections, each having at least one diameter and a substantially common longitudinal axis. A perfusate is introduced into the first chamber by an inlet. A UV radiation-emitting device is disposed inside the second chamber for providing irradiation to the perfusate. Irradiated perfusate is removed from the tank by an outlet. Other apparatuses and systems are described and methods for inactivating micro organisms by performing EVP and irradiating the perfusate.
1. An ex-vivo organ perfusion system comprising:
an organ chamber for containing an organ during perfusion of the organ;
a perfusion circuit for flowing perfusate to the organ chamber; and
an irradiation system for irradiating perfusate flowing through the perfusion circuit, the irradiation system comprising:
an apparatus comprising:
a first unit comprising side panels,
a second unit pivotally mounted on the first unit such that the second unit is moveable with respect to the first unit to open and close the apparatus, and
at least one radiation source mounted on at least one of the first and second units; and
a receptacle positioned in a chamber defined by the second and first units, wherein the receptacle is a continuous tube configured for flowing perfusate through the chamber for the at least one radiation source to irradiate the perfusate as the perfusate flows through the continuous tube, wherein the continuous tube comprises an inlet and an outlet and a constant diameter body portion extending from the inlet to the outlet, and wherein each of the side panels of the first unit defines a groove adapted to support the continuous diameter body portion of the receptacle.
2. The ex-vivo organ perfusion system of claim 1 , wherein the at least one radiation source comprises an ultraviolet lamp.
3. The ex-vivo organ perfusion system of claim 1 , further comprising safety sensors for detecting when the second unit is moved, such that the sensors detect when the irradiator is open and/or closed and for preventing the system from being inadvertently activated while the second unit is open.
4. The ex-vivo organ perfusion system of claim 1 , wherein at least one radiation source is mounted on each of the first and second units.
5. The ex-vivo organ perfusion system of claim 2 , wherein the ultraviolet lamp comprises one of: ultraviolet-A, ultraviolet-B and ultraviolet-C.
6. The ex-vivo organ perfusion system of claim 3 , wherein the safety sensors comprise sensing plates positioned on the first and second units.
7. The ex-vivo organ perfusion system of claim 1 , wherein ends of the inlet and outlet of the receptacle are machined for fitting external tubes to the ends.
8. The ex-vivo organ perfusion system of claim 1 , wherein the continuous tube is a quartz tube.
9. The ex-vivo organ perfusion system of claim 8 , wherein a wall thickness of the quartz tube is between 12.6 mm and 30.2 mm.
10. The ex-vivo organ perfusion system of claim 1 , wherein a joint between the first unit and the second unit bisects the apparatus.
11. The ex-vivo organ perfusion system of claim 1 , wherein the first unit and the second unit comprise an equal number of the one or more radiation sources.
12. The ex-vivo organ perfusion system of claim 1 , further comprising:
a male connector comprising a first tube-receiving opening configured to receive the inlet of the continuous tube to connect the inlet to the perfusion circuit; and
a female connector comprising a second tube-receiving opening configured to receive the outlet of the continuous tube to connect the outlet to the perfusion circuit.
13. The irradiation system of claim 12 , wherein:
the male connector comprises a first stop-flow pin configured to prevent the perfusate from flowing from the perfusion circuit into the inlet of the continuous tube, and
the female connector comprises a second stop-flow pin configured to prevent the perfusate from flowing into the perfusion circuit from the outlet of the continuous tube.