Lipid nanoparticle compositions and methods for mRNA delivery
Disclosed herein are compositions and methods for modulating the production of a protein in a target cell. The compositions and methods disclosed herein are capable of ameliorating diseases associated with protein or enzyme deficiencies.
1. A method for pulmonary delivery of messenger RNA (mRNA) for in vivo production of a protein in the lung comprising administering via inhalation from a nebulizer to the lung of a subject, a composition comprising an mRNA that encodes the protein,
wherein the mRNA is encapsulated within a lipid nanoparticle comprising one or more PEG-modified lipids, and
wherein the administering of the composition results in detectable levels of the protein at least 72 hours after administration in the subject's lung.
2. The method of claim 1 , wherein the mRNA comprises a 5′ untranslated region.
3. The method of claim 2 , wherein the mRNA comprises a 3′ untranslated region.
4. The method of claim 3 , wherein the mRNA comprises a cap structure.
5. The method of claim 4 , wherein the mRNA comprises a poly A tail.
6. The method of claim 5 , wherein the lipid nanoparticle comprises one or more cationic lipids.
7. The method of claim 6 , wherein the lipid nanoparticle comprises one or more non-cationic lipids.
8. The method of claim 7 , wherein the mRNA is unmodified.
9. The method of claim 8 , wherein the composition is a reconstituted lyophilized composition.
10. The method of claim 7 , wherein the modification comprises a modified nucleotide.
11. The method of claim 10 , wherein the modified nucleotide is pseudouridine.
12. The method of claim 11 , wherein the composition is a reconstituted lyophilized composition.
13. The method of claim 1 , wherein the protein encoded by the mRNA is a polypeptide.
14. The method of claim 1 , wherein the protein encoded by the mRNA is a peptide.
15. The method of claim 8 , wherein the lipid nanoparticle has a size of less than about 100 nm.
16. The method of claim 11 , wherein the lipid nanoparticle has a size of less than about 100 nm.
17. The method of claim 8 , wherein the one or more PEG-modified lipids constitute from 0.1% to 20% of the total lipids by a molar ratio.
18. The method of claim 11 , wherein the one or more PEG-modified lipids constitute from 0.1% to 20% of the total lipids by a molar ratio.
19. The method of claim 5 , wherein the poly A tail comprises at least 90 nucleotides.
20. The method of claim 5 , wherein the poly A tail comprises at least 500 nucleotides.