Long-chain dibasic acid with low content of hydroxyl acid impurity and production method thereof
The present invention relates to a long-chain dibasic acid with low content of hydroxyl acid impurity and a production method thereof, in particular to a method for producing a long-chain dibasic acid with low content of hydroxyl acid impurity by fermenting a long-chain dibasic acid producing strain prepared by homologous recombination method. The present invention relates to a recombinant long-chain dibasic acid producing microorganism, having increased alcohol dehydrogenase activity and optionally decreased acyl-CoA oxidase activity. The present invention also relates to a method of producing a long-chain dibasic acid by the recombinant long-chain dibasic acid producing microorganism and use thereof.
1. A product, which is
a recombinant long-chain dibasic acid producing microorganism, having increased alcohol dehydrogenase activity and decreased acyl-CoA oxidase activity, wherein, in the recombinant microorganism, PDX4 gene is replaced with one or more copies of ADH gene, wherein the recombinant long-chain dibasic acid producing microorganism produces a long-chain dibasic acid with a low content of hydroxyl acid impurity.
2. The product of claim 1 , wherein the recombinant long-chain dibasic acid producing microorganism:
(i) is selected from the group consisting of Corynebacterium, Geotrichum candidum, Candida, Pichia, Rhodotroula, Saccharomyces and Yarrowia;
(ii) is yeast; or
(iii) is Candida tropicalis or Candida sake.
3. The product of claim 1 , wherein in the recombinant microorganism one copy of PDX4 gene in the genome is replaced with one copy of ADH gene.
4. The product of claim 2 , wherein the nucleotide sequence of the ADH gene is set forth in SEQ ID NO: 3.
5. The product of claim 1 , wherein the long-chain dibasic acid is:
(i) selected from the group consisting of C9 to C22 long-chain dibasic acids;
(ii) selected from the group consisting of C9 to C18 long-chain dibasic acids;
(iii) one or more selected from the group consisting of C10 dibasic acid, C11 dibasic acid, C12 dibasic acid, C13 dibasic acid, C14 dibasic acid, C15 dibasic acid and C16 dibasic acid;
(iv) at least one or more of C10 to C16 dibasic acids, or at least one or more of n-C10 to C16 dibasic acids; or
(v) at least one or more selected from the group consisting of sebacic acid, undecanedioic acid, dodecanedioic acid, tridecanedioic acid, tetradecanedioic acid, pentadecanedioic acid and hexadecanedioic acid.
6. The product of claim 1 , wherein the content of hydroxyl acid impurity in the long-chain dibasic acid is more than 0 and less than 10,000 ppm, 4,000 ppm, 300 ppm or less, wherein the hydroxyl acid impurity comprises a hydroxyl fatty acid having one carboxyl group.
7. The product of claim 1 , wherein recombinant long-chain dibasic acid producing microorganism produces a fermentation broth in a process for producing a long-chain dibasic acid by fermentation, wherein the fermentation broth contains hydroxyl acid impurity, wherein the content of the hydroxyl acid impurity is less than 3%, 2%, 1.5%, 1.3%, 1.2%, 1.1%, 1.0%, 0.9%, 0.8%, 0.7%, 0.6%, 0.5%, 0.4%, 0.3% or less, wherein the percentage is the mass percentage of the hydroxyl acid impurity to the long-chain dibasic acid in the fermentation broth.