Synthesis of polyphosphorylated molecules from polyphosphates
Metaphosphate compounds can directly phosphorylate other compounds to provide easy synthetic access to phosphorylated compounds, including cyclic phosphate compounds.
1. A method of polyphosphorylating a compound comprising contacting the compound with an activated triphosphate including a phosphonium moiety, wherein the compound includes a nucleophilic group and is polyphosphoryled in a single step; and the phosphonium moiety consists of tripyrrolidinophosphonium.
2. The method of claim 1 , wherein the activated triphosphate is a cyclic phosphate.
3. The method of claim 1 , wherein an intermediate of the activated triphosphate is a trimetaphosphate 7-azabenzotriazole phosphoester or a trimetaphosphate benzotriazol-1-yl-oxy phosphoester.
4. The method of claim 1 , wherein the compound is a protic nucleophile (HNuc), wherein the HNuc includes an alcohol, an amine, or a thio compound.
5. The method of claim 1 , wherein the compound is a biochemical substrate, including a nucleoside, an amino acid, a sugar, or a fatty acid.
6. The method of claim 1 , wherein the compound is a carbonyl-containing compound.